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Title: Advances and perspectives on the use of CRISPR/Cas9 systems in plant genomics research

Genome editing with site-specific nucleases has become a powerful tool for functional characterization of plant genes and genetic improvement of agricultural crops. Among the various site-specific nuclease-based technologies available for genome editing, the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) systems have shown the greatest potential for rapid and efficient editing of genomes in plant species. Here, this article reviews the current status of application of CRISPR/Cas9 to plant genomics research, with a focus on loss-of-function and gain-of-function analysis of individual genes in the context of perennial plants and the potential application of CRISPR/Cas9 to perturbation of gene expression, as well as identification and analysis of gene modules as part of an accelerated domestication and synthetic biology effort.
Authors:
 [1] ;  [1] ;  [1] ;  [1] ;  [1]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
Publication Date:
Grant/Contract Number:
AC05-00OR22725; SC0008834
Type:
Accepted Manuscript
Journal Name:
Current Opinion in Plant Biology
Additional Journal Information:
Journal Volume: 30; Journal Issue: C; Journal ID: ISSN 1369-5266
Publisher:
Elsevier
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE Office of Science (SC)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1355869
Alternate Identifier(s):
OSTI ID: 1352955

Liu, Degao, Hu, Rongbin, Palla, Kaitlin J., Tuskan, Gerald A., and Yang, Xiaohan. Advances and perspectives on the use of CRISPR/Cas9 systems in plant genomics research. United States: N. p., Web. doi:10.1016/j.pbi.2016.01.007.
Liu, Degao, Hu, Rongbin, Palla, Kaitlin J., Tuskan, Gerald A., & Yang, Xiaohan. Advances and perspectives on the use of CRISPR/Cas9 systems in plant genomics research. United States. doi:10.1016/j.pbi.2016.01.007.
Liu, Degao, Hu, Rongbin, Palla, Kaitlin J., Tuskan, Gerald A., and Yang, Xiaohan. 2016. "Advances and perspectives on the use of CRISPR/Cas9 systems in plant genomics research". United States. doi:10.1016/j.pbi.2016.01.007. https://www.osti.gov/servlets/purl/1355869.
@article{osti_1355869,
title = {Advances and perspectives on the use of CRISPR/Cas9 systems in plant genomics research},
author = {Liu, Degao and Hu, Rongbin and Palla, Kaitlin J. and Tuskan, Gerald A. and Yang, Xiaohan},
abstractNote = {Genome editing with site-specific nucleases has become a powerful tool for functional characterization of plant genes and genetic improvement of agricultural crops. Among the various site-specific nuclease-based technologies available for genome editing, the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) systems have shown the greatest potential for rapid and efficient editing of genomes in plant species. Here, this article reviews the current status of application of CRISPR/Cas9 to plant genomics research, with a focus on loss-of-function and gain-of-function analysis of individual genes in the context of perennial plants and the potential application of CRISPR/Cas9 to perturbation of gene expression, as well as identification and analysis of gene modules as part of an accelerated domestication and synthetic biology effort.},
doi = {10.1016/j.pbi.2016.01.007},
journal = {Current Opinion in Plant Biology},
number = C,
volume = 30,
place = {United States},
year = {2016},
month = {2}
}