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Title: Early-branching gut fungi possess a large, comprehensive array of biomass-degrading enzymes

Abstract

The fungal kingdom is the source of almost all industrial enzymes in use for lignocellulose bioprocessing. Here we developed a systems-level approach that integrates transcriptomic sequencing, proteomics, phenotype, and biochemical studies of relatively unexplored basal fungi. Anaerobic gut fungi isolated from herbivores produce a large array of biomass-degrading enzymes that synergistically degrade crude, untreated plant biomass and are competitive with optimized commercial preparations from Aspergillus and Trichoderma. Compared to these model platforms, gut fungal enzymes are unbiased in substrate preference due to a wealth of xylan-degrading enzymes. These enzymes are universally catabolite-repressed and are further regulated by a rich landscape of noncoding regulatory RNAs. Additionally, we identified several promising sequence-divergent enzyme candidates for lignocellulosic bioprocessing.

Authors:
; ; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Univ. of California, Santa Barbara, CA (United States); USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); United States Dept. of Agriculture (USDA); US Army Research Office (ARO)
OSTI Identifier:
1355842
Alternate Identifier(s):
OSTI ID: 1485176
Grant/Contract Number:  
SC0010352; AC02-05CH11231; AC05-76RL01830; 2011-67017-20459; W911NF-09-0001
Resource Type:
Published Article
Journal Name:
Science
Additional Journal Information:
Journal Name: Science Journal Volume: 351 Journal Issue: 6278; Journal ID: ISSN 0036-8075
Publisher:
American Association for the Advancement of Science (AAAS)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Solomon, K. V., Haitjema, C. H., Henske, J. K., Gilmore, S. P., Borges-Rivera, D., Lipzen, A., Brewer, H. M., Purvine, S. O., Wright, A. T., Theodorou, M. K., Grigoriev, I. V., Regev, A., Thompson, D. A., and OMalley, M. A. Early-branching gut fungi possess a large, comprehensive array of biomass-degrading enzymes. United States: N. p., 2016. Web. doi:10.1126/science.aad1431.
Solomon, K. V., Haitjema, C. H., Henske, J. K., Gilmore, S. P., Borges-Rivera, D., Lipzen, A., Brewer, H. M., Purvine, S. O., Wright, A. T., Theodorou, M. K., Grigoriev, I. V., Regev, A., Thompson, D. A., & OMalley, M. A. Early-branching gut fungi possess a large, comprehensive array of biomass-degrading enzymes. United States. doi:10.1126/science.aad1431.
Solomon, K. V., Haitjema, C. H., Henske, J. K., Gilmore, S. P., Borges-Rivera, D., Lipzen, A., Brewer, H. M., Purvine, S. O., Wright, A. T., Theodorou, M. K., Grigoriev, I. V., Regev, A., Thompson, D. A., and OMalley, M. A. Thu . "Early-branching gut fungi possess a large, comprehensive array of biomass-degrading enzymes". United States. doi:10.1126/science.aad1431.
@article{osti_1355842,
title = {Early-branching gut fungi possess a large, comprehensive array of biomass-degrading enzymes},
author = {Solomon, K. V. and Haitjema, C. H. and Henske, J. K. and Gilmore, S. P. and Borges-Rivera, D. and Lipzen, A. and Brewer, H. M. and Purvine, S. O. and Wright, A. T. and Theodorou, M. K. and Grigoriev, I. V. and Regev, A. and Thompson, D. A. and OMalley, M. A.},
abstractNote = {The fungal kingdom is the source of almost all industrial enzymes in use for lignocellulose bioprocessing. Here we developed a systems-level approach that integrates transcriptomic sequencing, proteomics, phenotype, and biochemical studies of relatively unexplored basal fungi. Anaerobic gut fungi isolated from herbivores produce a large array of biomass-degrading enzymes that synergistically degrade crude, untreated plant biomass and are competitive with optimized commercial preparations from Aspergillus and Trichoderma. Compared to these model platforms, gut fungal enzymes are unbiased in substrate preference due to a wealth of xylan-degrading enzymes. These enzymes are universally catabolite-repressed and are further regulated by a rich landscape of noncoding regulatory RNAs. Additionally, we identified several promising sequence-divergent enzyme candidates for lignocellulosic bioprocessing.},
doi = {10.1126/science.aad1431},
journal = {Science},
number = 6278,
volume = 351,
place = {United States},
year = {2016},
month = {2}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1126/science.aad1431

Citation Metrics:
Cited by: 42 works
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