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Title: Ubiquitin facilitates a quality-control pathway that removes damaged chloroplasts

Abstract

Energy production by chloroplasts and mitochondria causes constant oxidative damage. A functioning photosynthetic cell requires quality-control mechanisms to turn over and degrade chloroplasts damaged by reactive oxygen species (ROS). Here in this study, we generated a conditionally lethal Arabidopsis mutant that accumulated excess protoporphyrin IX in the chloroplast and produced singlet oxygen. Damaged chloroplasts were subsequently ubiquitinated and selectively degraded. A genetic screen identified the plant U-box 4 (PUB4) E3 ubiquitin ligase as being necessary for this process. pub4-6 mutants had defects in stress adaptation and longevity. As a result, we have identified a signal that leads to the targeted removal of ROS-overproducing chloroplasts.

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
The Salk Inst., La Jolla, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Institutes of Health (NIH); German Research Foundation (DFG)
OSTI Identifier:
1355831
Alternate Identifier(s):
OSTI ID: 1345047
Grant/Contract Number:  
FG02-04ER15540; CA014195; NS072031
Resource Type:
Published Article
Journal Name:
Science
Additional Journal Information:
Journal Name: Science Journal Volume: 350 Journal Issue: 6259; Journal ID: ISSN 0036-8075
Publisher:
American Association for the Advancement of Science (AAAS)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Woodson, J. D., Joens, M. S., Sinson, A. B., Gilkerson, J., Salome, P. A., Weigel, D., Fitzpatrick, J. A., and Chory, J. Ubiquitin facilitates a quality-control pathway that removes damaged chloroplasts. United States: N. p., 2015. Web. doi:10.1126/science.aac7444.
Woodson, J. D., Joens, M. S., Sinson, A. B., Gilkerson, J., Salome, P. A., Weigel, D., Fitzpatrick, J. A., & Chory, J. Ubiquitin facilitates a quality-control pathway that removes damaged chloroplasts. United States. doi:10.1126/science.aac7444.
Woodson, J. D., Joens, M. S., Sinson, A. B., Gilkerson, J., Salome, P. A., Weigel, D., Fitzpatrick, J. A., and Chory, J. Thu . "Ubiquitin facilitates a quality-control pathway that removes damaged chloroplasts". United States. doi:10.1126/science.aac7444.
@article{osti_1355831,
title = {Ubiquitin facilitates a quality-control pathway that removes damaged chloroplasts},
author = {Woodson, J. D. and Joens, M. S. and Sinson, A. B. and Gilkerson, J. and Salome, P. A. and Weigel, D. and Fitzpatrick, J. A. and Chory, J.},
abstractNote = {Energy production by chloroplasts and mitochondria causes constant oxidative damage. A functioning photosynthetic cell requires quality-control mechanisms to turn over and degrade chloroplasts damaged by reactive oxygen species (ROS). Here in this study, we generated a conditionally lethal Arabidopsis mutant that accumulated excess protoporphyrin IX in the chloroplast and produced singlet oxygen. Damaged chloroplasts were subsequently ubiquitinated and selectively degraded. A genetic screen identified the plant U-box 4 (PUB4) E3 ubiquitin ligase as being necessary for this process. pub4-6 mutants had defects in stress adaptation and longevity. As a result, we have identified a signal that leads to the targeted removal of ROS-overproducing chloroplasts.},
doi = {10.1126/science.aac7444},
journal = {Science},
number = 6259,
volume = 350,
place = {United States},
year = {2015},
month = {10}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1126/science.aac7444

Citation Metrics:
Cited by: 21 works
Citation information provided by
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