Trimeric HIV-1-Env Structures Define Glycan Shields from Clades A, B, and G

Stewart-Jones, Guillaume B. E.; Soto, Cinque; Lemmin, Thomas; Chuang, Gwo-Yu; Druz, Aliaksandr; Kong, Rui; Thomas, Paul V.; Wagh, Kshitij; Zhou, Tongqing; Behrens, Anna-Janina; Bylund, Tatsiana; Choi, Chang W.; Davison, Jack R.; Georgiev, Ivelin S.; Joyce, M. Gordon; Kwon, Young Do; Pancera, Marie; Taft, Justin; Yang, Yongping; Zhang, Baoshan; Shivatare, Sachin S.; Shivatare, Vidya S.; Lee, Chang-Chun D.; Wu, Chung-Yi; Bewley, Carole A.; Burton, Dennis R.; Koff, Wayne C.; Connors, Mark; Crispin, Max; Baxa, Ulrich; Korber, Bette T.; Wong, Chi-Huey; Mascola, John R.; Kwong, Peter D.

doi:10.1016/j.cell.2016.04.010

Title: Trimeric HIV-1-Env Structures Define Glycan Shields from Clades A, B, and G

Authors:: Stewart-Jones, Guillaume B. E.; Soto, Cinque; Lemmin, Thomas; Chuang, Gwo-Yu; Druz, Aliaksandr; Kong, Rui; Thomas, Paul V.; Wagh, Kshitij; Zhou, Tongqing; Behrens, Anna-Janina; Bylund, Tatsiana; Choi, Chang W.; Davison, Jack R.; Georgiev, Ivelin S.; Joyce, M. Gordon; Kwon, Young Do; Pancera, Marie; Taft, Justin; Yang, Yongping; Zhang, Baoshan more »« less

Publication Date:: 2016-05-01

Research Org.:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Inst. of Health; Academia Sinica and Ministry of Science and Technology; Duke CHAVI-ID; Scripps Center For HIV/AIDS Vaccine Immunology-Immunogen Discovery (CHAVI-ID)

OSTI Identifier:: 1355607

Alternate Identifier(s):: OSTI ID: 1256368; OSTI ID: 1325284

Grant/Contract Number:: W-31-109-Eng-38; HHSN261200800001E; R01 AI072155; 104-0210-01-09-02; 103-2321-B-001-004; UM1 AI100645; 1UM1AI100663

Resource Type:: Published Article

Journal Name:: Cell

Additional Journal Information:: Journal Name: Cell Journal Volume: 165 Journal Issue: 4; Journal ID: ISSN 0092-8674

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Stewart-Jones, Guillaume B. E., Soto, Cinque, Lemmin, Thomas, Chuang, Gwo-Yu, Druz, Aliaksandr, Kong, Rui, Thomas, Paul V., Wagh, Kshitij, Zhou, Tongqing, Behrens, Anna-Janina, Bylund, Tatsiana, Choi, Chang W., Davison, Jack R., Georgiev, Ivelin S., Joyce, M. Gordon, Kwon, Young Do, Pancera, Marie, Taft, Justin, Yang, Yongping, Zhang, Baoshan, Shivatare, Sachin S., Shivatare, Vidya S., Lee, Chang-Chun D., Wu, Chung-Yi, Bewley, Carole A., Burton, Dennis R., Koff, Wayne C., Connors, Mark, Crispin, Max, Baxa, Ulrich, Korber, Bette T., Wong, Chi-Huey, Mascola, John R., and Kwong, Peter D.. Trimeric HIV-1-Env Structures Define Glycan Shields from Clades A, B, and G.  United States: N. p., 2016. 
Web.  doi:10.1016/j.cell.2016.04.010.

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                    Stewart-Jones, Guillaume B. E., Soto, Cinque, Lemmin, Thomas, Chuang, Gwo-Yu, Druz, Aliaksandr, Kong, Rui, Thomas, Paul V., Wagh, Kshitij, Zhou, Tongqing, Behrens, Anna-Janina, Bylund, Tatsiana, Choi, Chang W., Davison, Jack R., Georgiev, Ivelin S., Joyce, M. Gordon, Kwon, Young Do, Pancera, Marie, Taft, Justin, Yang, Yongping, Zhang, Baoshan, Shivatare, Sachin S., Shivatare, Vidya S., Lee, Chang-Chun D., Wu, Chung-Yi, Bewley, Carole A., Burton, Dennis R., Koff, Wayne C., Connors, Mark, Crispin, Max, Baxa, Ulrich, Korber, Bette T., Wong, Chi-Huey, Mascola, John R., & Kwong, Peter D.. Trimeric HIV-1-Env Structures Define Glycan Shields from Clades A, B, and G.  United States.  https://doi.org/10.1016/j.cell.2016.04.010

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                    Stewart-Jones, Guillaume B. E., Soto, Cinque, Lemmin, Thomas, Chuang, Gwo-Yu, Druz, Aliaksandr, Kong, Rui, Thomas, Paul V., Wagh, Kshitij, Zhou, Tongqing, Behrens, Anna-Janina, Bylund, Tatsiana, Choi, Chang W., Davison, Jack R., Georgiev, Ivelin S., Joyce, M. Gordon, Kwon, Young Do, Pancera, Marie, Taft, Justin, Yang, Yongping, Zhang, Baoshan, Shivatare, Sachin S., Shivatare, Vidya S., Lee, Chang-Chun D., Wu, Chung-Yi, Bewley, Carole A., Burton, Dennis R., Koff, Wayne C., Connors, Mark, Crispin, Max, Baxa, Ulrich, Korber, Bette T., Wong, Chi-Huey, Mascola, John R., and Kwong, Peter D.. Sun .  
"Trimeric HIV-1-Env Structures Define Glycan Shields from Clades A, B, and G".  United States.  https://doi.org/10.1016/j.cell.2016.04.010.

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@article{osti_1355607,

  title        = {Trimeric HIV-1-Env Structures Define Glycan Shields from Clades A, B, and G},

  author       = {Stewart-Jones, Guillaume B. E. and Soto, Cinque and Lemmin, Thomas and Chuang, Gwo-Yu and Druz, Aliaksandr and Kong, Rui and Thomas, Paul V. and Wagh, Kshitij and Zhou, Tongqing and Behrens, Anna-Janina and Bylund, Tatsiana and Choi, Chang W. and Davison, Jack R. and Georgiev, Ivelin S. and Joyce, M. Gordon and Kwon, Young Do and Pancera, Marie and Taft, Justin and Yang, Yongping and Zhang, Baoshan and Shivatare, Sachin S. and Shivatare, Vidya S. and Lee, Chang-Chun D. and Wu, Chung-Yi and Bewley, Carole A. and Burton, Dennis R. and Koff, Wayne C. and Connors, Mark and Crispin, Max and Baxa, Ulrich and Korber, Bette T. and Wong, Chi-Huey and Mascola, John R. and Kwong, Peter D.},

  abstractNote = {},

  doi          = {10.1016/j.cell.2016.04.010},

  journal      = {Cell},

  number       = 4,

  volume       = 165,

  place        = {United States},

  year         = {2016},

  month        = {5}

}

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Title: Trimeric HIV-1-Env Structures Define Glycan Shields from Clades A, B, and G

Citation Formats

Affinity Maturation of a Potent Family of HIV Antibodies Is Primarily Focused on Accommodating or Avoiding Glycans journal, December 2015

Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env journal, June 2015

Structure and immune recognition of trimeric pre-fusion HIV-1 Env journal, October 2014

Predominant Autoantibody Production by Early Human B Cell Precursors journal, September 2003

The HIV-1 Envelope Glycoproteins: Fusogens, Antigens, and Immunogens journal, June 1998

Immunization for HIV-1 Broadly Neutralizing Antibodies in Human Ig Knockin Mice journal, June 2015

The Broadly Neutralizing Anti-Human Immunodeficiency Virus Type 1 Antibody 2G12 Recognizes a Cluster of 1->2 Mannose Residues on the Outer Face of gp120 journal, July 2002

Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9 journal, November 2011

Restricting HIV-1 pathways for escape using rationally designed anti–HIV-1 antibodies journal, May 2013

The Mannose-Dependent Epitope for Neutralizing Antibody 2G12 on Human Immunodeficiency Virus Type 1 Glycoprotein gp120 journal, July 2002

Design and structure of two HIV-1 clade C SOSIP.664 trimers that increase the arsenal of native-like Env immunogens journal, September 2015

A Next-Generation Cleaved, Soluble HIV-1 Env Trimer, BG505 SOSIP.664 gp140, Expresses Multiple Epitopes for Broadly Neutralizing but Not Non-Neutralizing Antibodies journal, September 2013

Structure of the Epstein-Barr virus major envelope glycoprotein journal, October 2006

Crystal Structure of a Soluble Cleaved HIV-1 Envelope Trimer journal, October 2013

Rational HIV Immunogen Design to Target Specific Germline B Cell Receptors journal, March 2013

Proof of principle for epitope-focused vaccine design journal, February 2014

Cryo-EM Structure of a Fully Glycosylated Soluble Cleaved HIV-1 Envelope Trimer journal, October 2013

Galectin-1, -2, and -3 Exhibit Differential Recognition of Sialylated Glycans and Blood Group Antigens journal, April 2008

Assignment of intrachain disulfide bonds and characterization of potential glycosylation sites of the type 1 recombinant human immunodeficiency virus envelope glycoprotein (gp120) expressed in Chinese hamster ovary cells. journal, June 1990

Structures of HIV-1 Env V1V2 with broadly neutralizing antibodies reveal commonalities that enable vaccine design journal, December 2015

Arenavirus Glycan Shield Promotes Neutralizing Antibody Evasion and Protracted Infection journal, November 2015

Crystal structure of a fully glycosylated HIV-1 gp120 core reveals a stabilizing role for the glycan at Asn262: Stabilizing Role for the Glycan at Asn262 journal, January 2015

Assembly of Asparagine-Linked Oligosaccharides journal, June 1985

HIV-1 neutralizing antibodies induced by native-like envelope trimers journal, June 2015

Model Building and Refinement of a Natively Glycosylated HIV-1 Env Protein by High-Resolution Cryoelectron Microscopy journal, October 2015

Accurate bond and angle parameters for X-ray protein structure refinement journal, July 1991

Vaccine-Elicited Tier 2 HIV-1 Neutralizing Antibodies Bind to Quaternary Epitopes Involving Glycan-Deficient Patches Proximal to the CD4 Binding Site journal, May 2015

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Structural Mechanism of Trimeric HIV-1 Envelope Glycoprotein Activation journal, July 2012

Broadly Neutralizing Antibody 8ANC195 Recognizes Closed and Open States of HIV-1 Env journal, September 2015

Immunogenicity of Stabilized HIV-1 Envelope Trimers with Reduced Exposure of Non-neutralizing Epitopes journal, December 2015

CD4-Induced Activation in a Soluble HIV-1 Env Trimer journal, July 2014

Modular synthesis of N-glycans and arrays for the hetero-ligand binding analysis of HIV antibodies journal, March 2016

N-Linked glycoprotein assembly. Evidence that oligosaccharide attachment occurs within the lumen of the endoplasmic reticulum. journal, April 1980

A Native-Like SOSIP.664 Trimer Based on an HIV-1 Subtype B env Gene journal, January 2015

Major glycoprotein antigens that induce antibodies in AIDS patients are encoded by HTLV-III journal, May 1985

The Hepatitis C Virus Glycan Shield and Evasion of the Humoral Immune Response journal, October 2011

Antibody neutralization and escape by HIV-1 journal, March 2003

Complex-type N-glycan recognition by potent broadly neutralizing HIV antibodies journal, October 2012

A Potent and Broad Neutralizing Antibody Recognizes and Penetrates the HIV Glycan Shield journal, October 2011

Structural Evolution of Glycan Recognition by a Family of Potent HIV Antibodies journal, September 2014

Structural Repertoire of HIV-1-Neutralizing Antibodies Targeting the CD4 Supersite in 14 Donors journal, June 2015

Cryo-EM structure of a native, fully glycosylated, cleaved HIV-1 envelope trimer journal, March 2016

Supersite of immune vulnerability on the glycosylated face of HIV-1 envelope glycoprotein gp120 journal, May 2013

Developmental pathway for potent V1V2-directed HIV-neutralizing antibodies journal, March 2014

Multidonor Analysis Reveals Structural Elements, Genetic Determinants, and Maturation Pathway for HIV-1 Neutralization by VRC01-Class Antibodies journal, August 2013

Priming a broadly neutralizing antibody response to HIV-1 using a germline-targeting immunogen journal, June 2015

Evolutionary Forces Shaping the Golgi Glycosylation Machinery: Why Cell Surface Glycans Are Universal to Living Cells journal, April 2011

Viral dynamics in human immunodeficiency virus type 1 infection journal, January 1995

Rates of Molecular Evolution in RNA Viruses: A Quantitative Phylogenetic Analysis journal, February 2002

Affinity Maturation of a Potent Family of HIV Antibodies Is Primarily Focused on Accommodating or Avoiding Glycans
journal, December 2015

Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env
journal, June 2015

Structure and immune recognition of trimeric pre-fusion HIV-1 Env
journal, October 2014

Predominant Autoantibody Production by Early Human B Cell Precursors
journal, September 2003

The HIV-1 Envelope Glycoproteins: Fusogens, Antigens, and Immunogens
journal, June 1998

Immunization for HIV-1 Broadly Neutralizing Antibodies in Human Ig Knockin Mice
journal, June 2015

The Broadly Neutralizing Anti-Human Immunodeficiency Virus Type 1 Antibody 2G12 Recognizes a Cluster of 1->2 Mannose Residues on the Outer Face of gp120
journal, July 2002

Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9
journal, November 2011

Restricting HIV-1 pathways for escape using rationally designed anti–HIV-1 antibodies
journal, May 2013

The Mannose-Dependent Epitope for Neutralizing Antibody 2G12 on Human Immunodeficiency Virus Type 1 Glycoprotein gp120
journal, July 2002

Design and structure of two HIV-1 clade C SOSIP.664 trimers that increase the arsenal of native-like Env immunogens
journal, September 2015

A Next-Generation Cleaved, Soluble HIV-1 Env Trimer, BG505 SOSIP.664 gp140, Expresses Multiple Epitopes for Broadly Neutralizing but Not Non-Neutralizing Antibodies
journal, September 2013

Structure of the Epstein-Barr virus major envelope glycoprotein
journal, October 2006

Crystal Structure of a Soluble Cleaved HIV-1 Envelope Trimer
journal, October 2013

Rational HIV Immunogen Design to Target Specific Germline B Cell Receptors
journal, March 2013

Proof of principle for epitope-focused vaccine design
journal, February 2014

Cryo-EM Structure of a Fully Glycosylated Soluble Cleaved HIV-1 Envelope Trimer
journal, October 2013

Galectin-1, -2, and -3 Exhibit Differential Recognition of Sialylated Glycans and Blood Group Antigens
journal, April 2008

Assignment of intrachain disulfide bonds and characterization of potential glycosylation sites of the type 1 recombinant human immunodeficiency virus envelope glycoprotein (gp120) expressed in Chinese hamster ovary cells.
journal, June 1990

Structures of HIV-1 Env V1V2 with broadly neutralizing antibodies reveal commonalities that enable vaccine design
journal, December 2015

Arenavirus Glycan Shield Promotes Neutralizing Antibody Evasion and Protracted Infection
journal, November 2015

Crystal structure of a fully glycosylated HIV-1 gp120 core reveals a stabilizing role for the glycan at Asn262: Stabilizing Role for the Glycan at Asn262
journal, January 2015

Assembly of Asparagine-Linked Oligosaccharides
journal, June 1985

HIV-1 neutralizing antibodies induced by native-like envelope trimers
journal, June 2015

Model Building and Refinement of a Natively Glycosylated HIV-1 Env Protein by High-Resolution Cryoelectron Microscopy
journal, October 2015

Accurate bond and angle parameters for X-ray protein structure refinement
journal, July 1991

Vaccine-Elicited Tier 2 HIV-1 Neutralizing Antibodies Bind to Quaternary Epitopes Involving Glycan-Deficient Patches Proximal to the CD4 Binding Site
journal, May 2015

Glycan clustering stabilizes the mannose patch of HIV-1 and preserves vulnerability to broadly neutralizing antibodies
journal, June 2015

Structural Mechanism of Trimeric HIV-1 Envelope Glycoprotein Activation
journal, July 2012

Broadly Neutralizing Antibody 8ANC195 Recognizes Closed and Open States of HIV-1 Env
journal, September 2015

Immunogenicity of Stabilized HIV-1 Envelope Trimers with Reduced Exposure of Non-neutralizing Epitopes
journal, December 2015

CD4-Induced Activation in a Soluble HIV-1 Env Trimer
journal, July 2014

Modular synthesis of N-glycans and arrays for the hetero-ligand binding analysis of HIV antibodies
journal, March 2016

N-Linked glycoprotein assembly. Evidence that oligosaccharide attachment occurs within the lumen of the endoplasmic reticulum.
journal, April 1980

A Native-Like SOSIP.664 Trimer Based on an HIV-1 Subtype B env Gene
journal, January 2015

Major glycoprotein antigens that induce antibodies in AIDS patients are encoded by HTLV-III
journal, May 1985

The Hepatitis C Virus Glycan Shield and Evasion of the Humoral Immune Response
journal, October 2011

Antibody neutralization and escape by HIV-1
journal, March 2003

Complex-type N-glycan recognition by potent broadly neutralizing HIV antibodies
journal, October 2012

A Potent and Broad Neutralizing Antibody Recognizes and Penetrates the HIV Glycan Shield
journal, October 2011

Structural Evolution of Glycan Recognition by a Family of Potent HIV Antibodies
journal, September 2014

Structural Repertoire of HIV-1-Neutralizing Antibodies Targeting the CD4 Supersite in 14 Donors
journal, June 2015

Cryo-EM structure of a native, fully glycosylated, cleaved HIV-1 envelope trimer
journal, March 2016

Supersite of immune vulnerability on the glycosylated face of HIV-1 envelope glycoprotein gp120
journal, May 2013

Developmental pathway for potent V1V2-directed HIV-neutralizing antibodies
journal, March 2014

Multidonor Analysis Reveals Structural Elements, Genetic Determinants, and Maturation Pathway for HIV-1 Neutralization by VRC01-Class Antibodies
journal, August 2013

Priming a broadly neutralizing antibody response to HIV-1 using a germline-targeting immunogen
journal, June 2015

Evolutionary Forces Shaping the Golgi Glycosylation Machinery: Why Cell Surface Glycans Are Universal to Living Cells
journal, April 2011

Viral dynamics in human immunodeficiency virus type 1 infection
journal, January 1995

Rates of Molecular Evolution in RNA Viruses: A Quantitative Phylogenetic Analysis
journal, February 2002