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Title: Double-flow focused liquid injector for efficient serial femtosecond crystallography

Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Furthermore, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices.
Authors:
 [1] ;  [2] ;  [2] ;  [1] ;  [3] ;  [4] ;  [5] ;  [1] ;  [6] ;  [1] ;  [1] ;  [2] ;  [1] ;  [7] ;  [1] ;  [8] ;  [9] ;  [1] ;  [10] ;  [6] more »;  [6] ;  [11] ;  [11] ;  [12] ;  [12] ;  [13] ;  [13] ;  [14] ;  [4] ;  [15] ;  [16] ;  [16] ;  [17] ;  [4] ;  [4] ;  [4] ;  [4] ;  [12] ;  [18] ;  [6] ;  [3] ;  [6] ;  [11] ;  [19] ;  [1] « less
  1. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
  2. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany)
  3. Stanford Univ., Stanford, CA (United States)
  4. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  5. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Max-Planck-Institute for Biochemistry, Munich (Germany)
  6. Arizona State Univ., Tempe, AZ (United States)
  7. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Centre for Ultrafast Imaging, Hamburg (Germany)
  8. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); European XFEL GmbH, Hamburg (Germany)
  9. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany); Max-Planck Institute for the Structure and Dynamics of Matter, Hamburg (Germany)
  10. Arizona State Univ., Tempe, AZ (United States); Paul Scherrer Inst. (PSI), Villigen (Switzerland)
  11. Cornell Univ., Ithaca, NY (United States)
  12. Charite - Univ. Berlin, Berlin (Germany)
  13. Technische Univ. Berlin, Berlin (Germany)
  14. Hauptman-Woodward Medical Research Institute, Buffalo, NY (United States)
  15. Univ. of Nova Gorica, Nova Gorica (Slovenia); Institute of Metals and Technology, Ljubljana (Slovenia)
  16. Institute of Metals and Technology, Ljubljana (Slovenia)
  17. Helmholtz-Zentrum Geesthacht, Geesthacht (Germany)
  18. Univ. of Minnesota, Minneapolis, MN (United States)
  19. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany); Univ. of Minnesota, Minneapolis, MN (United States)
Publication Date:
Grant/Contract Number:
AC02-76SF00515
Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 7; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Research Org:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; fluid dynamics; nanocrystallography
OSTI Identifier:
1352549