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Title: Detection of Lipomannan in Cattle Infected with Bovine Tuberculosis

Early and rapid detection of bovine tuberculosis (bTB) is critical to controlling the spread of this disease in cattle and other animals. Here in this study, we demonstrate the development of an immunoassay for the direct detection of the bovine bTB biomarker, lipomannan (LM) in serum using a waveguide-based optical biosensor. We apply an ultra-sensitive detection strategy developed by our team, termed lipoprotein capture, that exploits the pull-down of high-density lipoprotein (HDL) nanodiscs from cattle blood that allows for the recovery and detection of associated LM. We also profile the change in the expression of these TB biomarkers as a function of time from a small set of samples collected from studies of bovine TB-infected cattle. Lastly, we demonstrate for the first time the direct detection of bovine LM in serum, and clearly show that the biomarker is expressed in detectable concentrations during the entire course of the infection.
Authors:
ORCiD logo [1] ;  [1] ;  [2] ;  [1] ; ORCiD logo [1]
  1. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  2. US Dept. of Agriculture (USDA)., Ames, IA (United States). National Animal Disease Center
Publication Date:
Report Number(s):
LA-UR-17-21083
Journal ID: ISSN 0910-6340
Grant/Contract Number:
AC52-06NA25396
Type:
Accepted Manuscript
Journal Name:
Analytical Sciences
Additional Journal Information:
Journal Volume: 33; Journal Issue: 4; Journal ID: ISSN 0910-6340
Publisher:
Japan Society for Analytical Chemistry
Research Org:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org:
USDOE Laboratory Directed Research and Development (LDRD) Program; New Mexico Small Business Assistance (NMSBA); US Dept. of Agriculture (USDA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Biological Science
OSTI Identifier:
1352430

Vu, Dung M., Sakamuri, Rama M., Waters, W. Ray, Swanson, Basil I., and Mukundan, Harshini. Detection of Lipomannan in Cattle Infected with Bovine Tuberculosis. United States: N. p., Web. doi:10.2116/analsci.33.457.
Vu, Dung M., Sakamuri, Rama M., Waters, W. Ray, Swanson, Basil I., & Mukundan, Harshini. Detection of Lipomannan in Cattle Infected with Bovine Tuberculosis. United States. doi:10.2116/analsci.33.457.
Vu, Dung M., Sakamuri, Rama M., Waters, W. Ray, Swanson, Basil I., and Mukundan, Harshini. 2017. "Detection of Lipomannan in Cattle Infected with Bovine Tuberculosis". United States. doi:10.2116/analsci.33.457. https://www.osti.gov/servlets/purl/1352430.
@article{osti_1352430,
title = {Detection of Lipomannan in Cattle Infected with Bovine Tuberculosis},
author = {Vu, Dung M. and Sakamuri, Rama M. and Waters, W. Ray and Swanson, Basil I. and Mukundan, Harshini},
abstractNote = {Early and rapid detection of bovine tuberculosis (bTB) is critical to controlling the spread of this disease in cattle and other animals. Here in this study, we demonstrate the development of an immunoassay for the direct detection of the bovine bTB biomarker, lipomannan (LM) in serum using a waveguide-based optical biosensor. We apply an ultra-sensitive detection strategy developed by our team, termed lipoprotein capture, that exploits the pull-down of high-density lipoprotein (HDL) nanodiscs from cattle blood that allows for the recovery and detection of associated LM. We also profile the change in the expression of these TB biomarkers as a function of time from a small set of samples collected from studies of bovine TB-infected cattle. Lastly, we demonstrate for the first time the direct detection of bovine LM in serum, and clearly show that the biomarker is expressed in detectable concentrations during the entire course of the infection.},
doi = {10.2116/analsci.33.457},
journal = {Analytical Sciences},
number = 4,
volume = 33,
place = {United States},
year = {2017},
month = {4}
}