COPII-coated membranes function as transport carriers of intracellular procollagen I
Abstract
The coat protein complex II (COPII) is essential for the transport of large cargo, such as 300-nm procollagen I (PC1) molecules, from the endoplasmic reticulum (ER) to the Golgi. Previous work has shown that the CUL3-KLHL12 complex increases the size of COPII vesicles at ER exit sites to more than 300 nm in diameter and accelerates the secretion of PC1. However, the role of large COPII vesicles as PC1 transport carriers was not unambiguously demonstrated. In this study, using stochastic optical reconstruction microscopy, correlated light electron microscopy, and live-cell imaging, we demonstrate the existence of mobile COPII-coated vesicles that completely encapsulate the cargo PC1 and are physically separated from ER. We also developed a cell-free COPII vesicle budding reaction that reconstitutes the capture of PC1 into large COPII vesicles. This process requires COPII proteins and the GTPase activity of the COPII subunit SAR1. We conclude that large COPII vesicles are bona fide carriers of PC1.
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1352291
- Resource Type:
- Published Article
- Journal Name:
- Journal of Cell Biology
- Additional Journal Information:
- Journal Name: Journal of Cell Biology Journal Volume: 216 Journal Issue: 6; Journal ID: ISSN 0021-9525
- Publisher:
- Rockefeller University Press
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Gorur, Amita, Yuan, Lin, Kenny, Samuel J., Baba, Satoshi, Xu, Ke, and Schekman, Randy. COPII-coated membranes function as transport carriers of intracellular procollagen I. United States: N. p., 2017.
Web. doi:10.1083/jcb.201702135.
Gorur, Amita, Yuan, Lin, Kenny, Samuel J., Baba, Satoshi, Xu, Ke, & Schekman, Randy. COPII-coated membranes function as transport carriers of intracellular procollagen I. United States. https://doi.org/10.1083/jcb.201702135
Gorur, Amita, Yuan, Lin, Kenny, Samuel J., Baba, Satoshi, Xu, Ke, and Schekman, Randy. Thu .
"COPII-coated membranes function as transport carriers of intracellular procollagen I". United States. https://doi.org/10.1083/jcb.201702135.
@article{osti_1352291,
title = {COPII-coated membranes function as transport carriers of intracellular procollagen I},
author = {Gorur, Amita and Yuan, Lin and Kenny, Samuel J. and Baba, Satoshi and Xu, Ke and Schekman, Randy},
abstractNote = {The coat protein complex II (COPII) is essential for the transport of large cargo, such as 300-nm procollagen I (PC1) molecules, from the endoplasmic reticulum (ER) to the Golgi. Previous work has shown that the CUL3-KLHL12 complex increases the size of COPII vesicles at ER exit sites to more than 300 nm in diameter and accelerates the secretion of PC1. However, the role of large COPII vesicles as PC1 transport carriers was not unambiguously demonstrated. In this study, using stochastic optical reconstruction microscopy, correlated light electron microscopy, and live-cell imaging, we demonstrate the existence of mobile COPII-coated vesicles that completely encapsulate the cargo PC1 and are physically separated from ER. We also developed a cell-free COPII vesicle budding reaction that reconstitutes the capture of PC1 into large COPII vesicles. This process requires COPII proteins and the GTPase activity of the COPII subunit SAR1. We conclude that large COPII vesicles are bona fide carriers of PC1.},
doi = {10.1083/jcb.201702135},
journal = {Journal of Cell Biology},
number = 6,
volume = 216,
place = {United States},
year = {Thu Apr 20 00:00:00 EDT 2017},
month = {Thu Apr 20 00:00:00 EDT 2017}
}
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