Predominance and metabolic potential of Halanaerobium spp. in produced water from hydraulically fractured Marcellus Shale wells
Abstract
Here, microbial activity in the produced water from hydraulically fractured oil and gas wells may potentially interfere with hydrocarbon production and cause damage to the well and surface infrastructure via corrosion, sulfide release, and fouling. In this study, we surveyed the microbial abundance and community structure of produced water sampled from 42 Marcellus Shale wells in southwestern Pennsylvania (well age ranged from 150 to 1,846 days) to better understand the microbial diversity of produced water. We sequenced the V4 region of the 16S rRNA gene to assess taxonomy and utilized quantitative PCR (qPCR) to evaluate the microbial abundance across all 42 produced water samples. Bacteria of the order Halanaerobiales were found to be the most abundant organisms in the majority of the produced water samples, emphasizing their previously suggested role in hydraulic fracturing-related microbial activity. Statistical analyses identified correlations between well age and biocide formulation and the microbial community, in particular, the relative abundance of Halanaerobiales. We further investigated the role of members of the order Halanaerobiales in produced water by reconstructing and annotating a Halanaerobium draft genome (named MDAL1), using shotgun metagenomic sequencing and metagenomic binning. The recovered draft genome was found to be closely related to the speciesmore »
- Authors:
-
[2]
- National Energy Technology Lab. (NETL), Pittsburgh, PA (United States); Univ. of Pittsburgh, Pittsburgh, PA (United States)
- Univ. of Pittsburgh, Pittsburgh, PA (United States)
- National Energy Technology Lab. (NETL), Pittsburgh, PA (United States); AECOM, Pittsburgh, PA (United States)
- National Energy Technology Lab. (NETL), Pittsburgh, PA (United States)
- Publication Date:
- Research Org.:
- National Energy Technology Lab. (NETL), Pittsburgh, PA, and Morgantown, WV (United States). In-house Research
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1351183
- Report Number(s):
- NETL-PUB-20135
Journal ID: ISSN 0099-2240
- Grant/Contract Number:
- RES1000027/183U
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Applied and Environmental Microbiology
- Additional Journal Information:
- Journal Volume: 83; Journal Issue: 8; Journal ID: ISSN 0099-2240
- Publisher:
- American Society for Microbiology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 04 OIL SHALES AND TAR SANDS; Marcellus Shale; biogeochemistry
Citation Formats
Lipus, Daniel, Vikram, Amit, Ross, Daniel, Bain, Daniel, Gulliver, Djuna, Hammack, Richard, and Bibby, Kyle. Predominance and metabolic potential of Halanaerobium spp. in produced water from hydraulically fractured Marcellus Shale wells. United States: N. p., 2017.
Web. doi:10.1128/AEM.02659-16.
Lipus, Daniel, Vikram, Amit, Ross, Daniel, Bain, Daniel, Gulliver, Djuna, Hammack, Richard, & Bibby, Kyle. Predominance and metabolic potential of Halanaerobium spp. in produced water from hydraulically fractured Marcellus Shale wells. United States. https://doi.org/10.1128/AEM.02659-16
Lipus, Daniel, Vikram, Amit, Ross, Daniel, Bain, Daniel, Gulliver, Djuna, Hammack, Richard, and Bibby, Kyle. Fri .
"Predominance and metabolic potential of Halanaerobium spp. in produced water from hydraulically fractured Marcellus Shale wells". United States. https://doi.org/10.1128/AEM.02659-16. https://www.osti.gov/servlets/purl/1351183.
@article{osti_1351183,
title = {Predominance and metabolic potential of Halanaerobium spp. in produced water from hydraulically fractured Marcellus Shale wells},
author = {Lipus, Daniel and Vikram, Amit and Ross, Daniel and Bain, Daniel and Gulliver, Djuna and Hammack, Richard and Bibby, Kyle},
abstractNote = {Here, microbial activity in the produced water from hydraulically fractured oil and gas wells may potentially interfere with hydrocarbon production and cause damage to the well and surface infrastructure via corrosion, sulfide release, and fouling. In this study, we surveyed the microbial abundance and community structure of produced water sampled from 42 Marcellus Shale wells in southwestern Pennsylvania (well age ranged from 150 to 1,846 days) to better understand the microbial diversity of produced water. We sequenced the V4 region of the 16S rRNA gene to assess taxonomy and utilized quantitative PCR (qPCR) to evaluate the microbial abundance across all 42 produced water samples. Bacteria of the order Halanaerobiales were found to be the most abundant organisms in the majority of the produced water samples, emphasizing their previously suggested role in hydraulic fracturing-related microbial activity. Statistical analyses identified correlations between well age and biocide formulation and the microbial community, in particular, the relative abundance of Halanaerobiales. We further investigated the role of members of the order Halanaerobiales in produced water by reconstructing and annotating a Halanaerobium draft genome (named MDAL1), using shotgun metagenomic sequencing and metagenomic binning. The recovered draft genome was found to be closely related to the species H. congolense, an oil field isolate, and Halanaerobium sp. strain T82-1, also recovered from hydraulic fracturing produced water. Reconstruction of metabolic pathways revealed Halanaerobium sp. strain MDAL1 to have the potential for acid production, thiosulfate reduction, and biofilm formation, suggesting it to have the ability to contribute to corrosion, souring, and biofouling events in the hydraulic fracturing infrastructure.},
doi = {10.1128/AEM.02659-16},
journal = {Applied and Environmental Microbiology},
number = 8,
volume = 83,
place = {United States},
year = {Fri Feb 03 00:00:00 EST 2017},
month = {Fri Feb 03 00:00:00 EST 2017}
}
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Works referenced in this record:
Environmental signals and regulatory pathways that influence biofilm formation: Signalling in biofilm formation
journal, April 2004
- Stanley, Nicola R.; Lazazzera, Beth A.
- Molecular Microbiology, Vol. 52, Issue 4
Osmotic Control of opuA Expression in Bacillus subtilis and Its Modulation in Response to Intracellular Glycine Betaine and Proline Pools
journal, November 2012
- Hoffmann, T.; Wensing, A.; Brosius, M.
- Journal of Bacteriology, Vol. 195, Issue 3
Genetic characterization of ompH mutants in the deep-sea bacterium Photobacterium sp. strain SS9
journal, November 1994
- Bartlett, Douglas; Chi, Ellen
- Archives of Microbiology, Vol. 162, Issue 5
Thiosulfate reduction, an important physiological feature shared by members of the order thermotogales.
journal, January 1995
- Ravot, G.; Ollivier, B.; Magot, M.
- Applied and environmental microbiology, Vol. 61, Issue 5
A Critical Review of the Risks to Water Resources from Unconventional Shale Gas Development and Hydraulic Fracturing in the United States
journal, March 2014
- Vengosh, Avner; Jackson, Robert B.; Warner, Nathaniel
- Environmental Science & Technology, Vol. 48, Issue 15
Complete genome sequence of the extremely halophilic Halanaerobium praevalens type strain (GSLT)
journal, June 2011
- Ivanova, Natalia; Sikorski, Johannes; Chertkov, Olga
- Standards in Genomic Sciences, Vol. 4, Issue 3
RNAmmer: consistent and rapid annotation of ribosomal RNA genes
journal, April 2007
- Lagesen, Karin; Hallin, Peter; Rødland, Einar Andreas
- Nucleic Acids Research, Vol. 35, Issue 9
Acetogenic Bacteria
book, January 2013
- Muller, Volker; Frerichs, Janin
- Encyclopedia of Life Sciences
Halophilic Acetogenic Bacteria
book, January 1994
- Zavarzin, George A.; Zhilina, Tatjana N.; Pusheva, Margarete A.
- Acetogenesis
Chaotropicity: a key factor in product tolerance of biofuel-producing microorganisms
journal, June 2015
- Cray, Jonathan A.; Stevenson, Andrew; Ball, Philip
- Current Opinion in Biotechnology, Vol. 33
Germination of spores of Bacillales and Clostridiales species: mechanisms and proteins involved
journal, February 2011
- Paredes-Sabja, Daniel; Setlow, Peter; Sarker, Mahfuzur R.
- Trends in Microbiology, Vol. 19, Issue 2
KEGG: Kyoto Encyclopedia of Genes and Genomes
journal, January 2000
- Kanehisa, Minoru; Goto, Susumu
- Nucleic Acids Research, Vol. 28, Issue 1, p. 27-30
Characterization of bacterial community associated to biofilms of corroded oil pipelines from the southeast of Mexico
journal, June 2006
- Neria-González, Isabel; Wang, En Tao; Ramírez, Florina
- Anaerobe, Vol. 12, Issue 3
Bacterial Communities Associated with Production Facilities of Two Newly Drilled Thermogenic Natural Gas Wells in the Barnett Shale (Texas, USA)
journal, May 2012
- Davis, James P.; Struchtemeyer, Christopher G.; Elshahed, Mostafa S.
- Microbial Ecology, Vol. 64, Issue 4
Metatranscriptome analysis of active microbial communities in produced water samples from the Marcellus Shale
journal, July 2016
- Vikram, Amit; Lipus, Daniel; Bibby, Kyle
- Microbial Ecology, Vol. 72, Issue 3
Energy metabolism in halophilic and alkaliphilic acetogenic bacteria
journal, January 2006
- Detkova, E. N.; Pusheva, M. A.
- Microbiology, Vol. 75, Issue 1
K+ Transport and its Role for Osmoregulation in a Halophilic Member of the Bacteria Domain: Characterization of the K+ Uptake Systems from Halomonas Elongata
book, January 2005
- Kunte, Hans Jörg
- Adaptation to Life at High Salt Concentrations in Archaea, Bacteria, and Eukarya
Microbial metabolisms in a 2.5-km-deep ecosystem created by hydraulic fracturing in shales
journal, September 2016
- Daly, Rebecca A.; Borton, Mikayla A.; Wilkins, Michael J.
- Nature Microbiology, Vol. 1, Issue 10
Quantitative real-time PCR using TaqMan and SYBR Green for Actinobacillus actinomycetemcomitans , Porphyromonas gingivalis , Prevotella intermedia , tetQ gene and total bacteria
journal, October 2003
- Maeda, Hiroshi; Fujimoto, Chiyo; Haruki, Yasuhiro
- FEMS Immunology & Medical Microbiology, Vol. 39, Issue 1
Anaerobic bacteria from hypersaline environments.
journal, January 1994
- Ollivier, B.; Caumette, P.; Garcia, J. L.
- Microbiological Reviews, Vol. 58, Issue 1
Biodegradation in Waters from Hydraulic Fracturing: Chemistry, Microbiology, and Engineering
journal, May 2014
- Strong, Lisa C.; Gould, Trevor; Kasinkas, Lisa
- Journal of Environmental Engineering, Vol. 140, Issue 5
Life at high salt concentrations, intracellular KCl concentrations, and acidic proteomes
journal, January 2013
- Oren, Aharon
- Frontiers in Microbiology, Vol. 4
Regional Variation in Water-Related Impacts of Shale Gas Development and Implications for Emerging International Plays
journal, April 2014
- Mauter, Meagan S.; Alvarez, Pedro J. J.; Burton, Allen
- Environmental Science & Technology, Vol. 48, Issue 15
Multiple Factors Modulate Biofilm Formation by the Anaerobic Pathogen Clostridium difficile
journal, November 2012
- Thapa, T.; Leuzzi, R.; Ng, Y. K.
- Journal of Bacteriology, Vol. 195, Issue 3
Prokaryotic Community Diversity Along an Increasing Salt Gradient in a Soda Ash Concentration Pond
journal, September 2015
- Simachew, Addis; Lanzén, Anders; Gessesse, Amare
- Microbial Ecology, Vol. 71, Issue 2
Water Management Challenges Associated with the Production of Shale Gas by Hydraulic Fracturing
journal, June 2011
- Gregory, K. B.; Vidic, R. D.; Dzombak, D. A.
- Elements, Vol. 7, Issue 3
Microbial life at high salt concentrations: phylogenetic and metabolic diversity
journal, January 2008
- Oren, Aharon
- Saline Systems, Vol. 4, Issue 1
Advances in Tools to Monitor Souring and Corrosion in Oil and Gas Fields
conference, April 2015
- An, Annie; An, Dongshan; Elliott, Alexander
- SPE International Symposium on Oilfield Chemistry
KEGG for integration and interpretation of large-scale molecular data sets
journal, November 2011
- Kanehisa, M.; Goto, S.; Sato, Y.
- Nucleic Acids Research, Vol. 40, Issue D1
The Functional Potential of Microbial Communities in Hydraulic Fracturing Source Water and Produced Water from Natural Gas Extraction Characterized by Metagenomic Sequencing
journal, October 2014
- Mohan, Arvind Murali; Bibby, Kyle J.; Lipus, Daniel
- PLoS ONE, Vol. 9, Issue 10
Biofilms, flagella, and mechanosensing of surfaces by bacteria
journal, September 2014
- Belas, Robert
- Trends in Microbiology, Vol. 22, Issue 9
Suggested Reporting Parameters for Investigations of Wastewater from Unconventional Shale Gas Extraction
journal, November 2013
- Bibby, Kyle J.; Brantley, Susan L.; Reible, Danny D.
- Environmental Science & Technology, Vol. 47, Issue 23
Microbial Dynamics and Control in Shale Gas Production
journal, November 2014
- Gaspar, Jason; Mathieu, Jacques; Yang, Yu
- Environmental Science & Technology Letters, Vol. 1, Issue 12
CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes
journal, May 2015
- Parks, Donovan H.; Imelfort, Michael; Skennerton, Connor T.
- Genome Research, Vol. 25, Issue 7
Influence of Salinity on the Bacterial Community Composition in Lake Bosten, a Large Oligosaline Lake in Arid Northwestern China
journal, April 2012
- Tang, Xiangming; Xie, Guijuan; Shao, Keqiang
- Applied and Environmental Microbiology, Vol. 78, Issue 13
Uptake and synthesis of compatible solutes as microbial stress responses to high-osmolality environments
journal, September 1998
- Kempf, Bettina; Bremer, E.
- Archives of Microbiology, Vol. 170, Issue 5
Temporal Changes in Microbial Ecology and Geochemistry in Produced Water from Hydraulically Fractured Marcellus Shale Gas Wells
journal, May 2014
- Cluff, Maryam A.; Hartsock, Angela; MacRae, Jean D.
- Environmental Science & Technology, Vol. 48, Issue 11
MaxBin: an automated binning method to recover individual genomes from metagenomes using an expectation-maximization algorithm
journal, August 2014
- Wu, Yu-Wei; Tang, Yung-Hsu; Tringe, Susannah G.
- Microbiome, Vol. 2, Issue 1
Microbial characterization of basalt formation waters targeted for geological carbon sequestration
journal, March 2013
- Lavalleur, Heather J.; Colwell, Frederick S.
- FEMS Microbiology Ecology, Vol. 85, Issue 1
rdlA, a new gene encoding a rhodanese-like protein in Halanaerobium congolense and other thiosulfate-reducing anaerobes
journal, December 2005
- Ravot, Gilles; Casalot, Laurence; Ollivier, Bernard
- Research in Microbiology, Vol. 156, Issue 10
Pseudomonas biofilm matrix composition and niche biology
journal, July 2012
- Mann, Ethan E.; Wozniak, Daniel J.
- FEMS Microbiology Reviews, Vol. 36, Issue 4
The RAST Server: Rapid Annotations using Subsystems Technology
journal, January 2008
- Aziz, Ramy K.; Bartels, Daniela; Best, Aaron A.
- BMC Genomics, Vol. 9, Issue 1, Article No. 75
Halanaerobium sehlinense sp. nov., an extremely halophilic, fermentative, strictly anaerobic bacterium from sediments of the hypersaline lake Sehline Sebkha
journal, October 2012
- Abdeljabbar, H.; Cayol, J. -L.; Ben Hania, W.
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 63, Issue Pt 6
Produced Water Exposure Alters Bacterial Response to Biocides
journal, October 2014
- Vikram, Amit; Lipus, Daniel; Bibby, Kyle
- Environmental Science & Technology, Vol. 48, Issue 21
Hydraulic fracturing offers view of microbial life in the deep terrestrial subsurface
journal, August 2016
- Mouser, Paula J.; Borton, Mikayla; Darrah, Thomas H.
- FEMS Microbiology Ecology, Vol. 92, Issue 11
A critical assessment of the efficacy of biocides used during the hydraulic fracturing process in shale natural gas wells
journal, July 2012
- Struchtemeyer, Christopher G.; Morrison, Michael D.; Elshahed, Mostafa S.
- International Biodeterioration & Biodegradation, Vol. 71
Genetic analysis of Escherichia coli biofilm formation: roles of flagella, motility, chemotaxis and type I pili
journal, October 1998
- Pratt, Leslie A.; Kolter, Roberto
- Molecular Microbiology, Vol. 30, Issue 2
Complete genome sequence of Acetohalobium arabaticum type strain (Z-7288T)
journal, August 2010
- Sikorski, Johannes; Lapidus, Alla; Chertkov, Olga
- Standards in Genomic Sciences, Vol. 3, Issue 1
SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing
journal, May 2012
- Bankevich, Anton; Nurk, Sergey; Antipov, Dmitry
- Journal of Computational Biology, Vol. 19, Issue 5
Genome Sequence of Halanaerobium saccharolyticum subsp. saccharolyticum Strain DSM 6643T, a Halophilic Hydrogen-Producing Bacterium
journal, February 2013
- Kivisto, A.; Larjo, A.; Ciranna, A.
- Genome Announcements, Vol. 1, Issue 2
Bacterial communities associated with hydraulic fracturing fluids in thermogenic natural gas wells in North Central Texas, USA
journal, November 2011
- Struchtemeyer, Christopher G.; Elshahed, Mostafa S.
- FEMS Microbiology Ecology, Vol. 81, Issue 1
Microbial diversity and methanogenic activity of Antrim Shale formation waters from recently fractured wells
journal, January 2013
- Wuchter, Cornelia; Banning, Erin; Mincer, Tracy J.
- Frontiers in Microbiology, Vol. 4
Microbial Community Changes in Hydraulic Fracturing Fluids and Produced Water from Shale Gas Extraction
journal, October 2013
- Murali Mohan, Arvind; Hartsock, Angela; Bibby, Kyle J.
- Environmental Science & Technology, Vol. 47, Issue 22
Potassium Transport in a Halophilic Member of the Bacteria Domain: Identification and Characterization of the K+ Uptake Systems TrkH and TrkI from Halomonas elongata DSM 2581T
journal, January 2005
- Kraegeloh, A.; Amendt, B.; Kunte, H. J.
- Journal of Bacteriology, Vol. 187, Issue 3
Pseudomonas biofilm formation and antibiotic resistance are linked to phenotypic variation
journal, April 2002
- Drenkard, Eliana; Ausubel, Frederick M.
- Nature, Vol. 416, Issue 6882
Accurate phylogenetic classification of variable-length DNA fragments
journal, December 2006
- McHardy, Alice Carolyn; Martín, Héctor García; Tsirigos, Aristotelis
- Nature Methods, Vol. 4, Issue 1
QIIME allows analysis of high-throughput community sequencing data
journal, April 2010
- Caporaso, J. Gregory; Kuczynski, Justin; Stombaugh, Jesse
- Nature Methods, Vol. 7, Issue 5
Biogenic versus Thermogenic H 2 S Source Determination in Bakken Wells: Considerations for Biocide Application
journal, March 2016
- Gaspar, Jason; Davis, Drew; Camacho, Carlos
- Environmental Science & Technology Letters, Vol. 3, Issue 4
Comparison of bacterial community in aqueous and oil phases of water-flooded petroleum reservoirs using pyrosequencing and clone library approaches
journal, January 2014
- Wang, Li-Ying; Ke, Wen-Ji; Sun, Xiao-Bo
- Applied Microbiology and Biotechnology, Vol. 98, Issue 9
Haloanaerobium congolense sp. nov., an anaerobic, moderately halophilic, thiosulfate- and sulfur-reducing bacterium from an African oil field
journal, February 1997
- Ravot, G.; Magot, M.; Ollivier, B.
- FEMS Microbiology Letters, Vol. 147, Issue 1
Biocides in Hydraulic Fracturing Fluids: A Critical Review of Their Usage, Mobility, Degradation, and Toxicity
journal, December 2014
- Kahrilas, Genevieve A.; Blotevogel, Jens; Stewart, Philip S.
- Environmental Science & Technology, Vol. 49, Issue 1
Complete Genome Sequence of the Haloalkaliphilic, Hydrogen-Producing Bacterium Halanaerobium hydrogeniformans
journal, May 2011
- Brown, S. D.; Begemann, M. B.; Mormile, M. R.
- Journal of Bacteriology, Vol. 193, Issue 14
The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST)
journal, November 2013
- Overbeek, Ross; Olson, Robert; Pusch, Gordon D.
- Nucleic Acids Research, Vol. 42, Issue D1
Organic and inorganic composition and microbiology of produced waters from Pennsylvania shale gas wells
journal, September 2015
- Akob, Denise M.; Cozzarelli, Isabelle M.; Dunlap, Darren S.
- Applied Geochemistry, Vol. 60
Metabolic Capability of a Predominant Halanaerobium sp. in Hydraulically Fractured Gas Wells and Its Implication in Pipeline Corrosion
journal, June 2016
- Liang, Renxing; Davidova, Irene A.; Marks, Christopher R.
- Frontiers in Microbiology, Vol. 7
A new anaerobic, sporing, acetate-oxidizing, sulfate-reducing bacterium, Desulfotomaculum (emend.) acetoxidans
journal, January 1977
- Widdel, Friedrich; Pfennig, Norbert
- Archives of Microbiology, Vol. 112, Issue 1
Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms
journal, March 2012
- Caporaso, J. Gregory; Lauber, Christian L.; Walters, William A.
- The ISME Journal, Vol. 6, Issue 8
Bacterial diguanylate cyclases: Structure, function and mechanism in exopolysaccharide biofilm development
journal, January 2015
- Whiteley, Chris G.; Lee, Duu-Jong
- Biotechnology Advances, Vol. 33, Issue 1
Iron and Oxidative Stress in Bacteria
journal, January 2000
- Touati, Danièle
- Archives of Biochemistry and Biophysics, Vol. 373, Issue 1
Phylogenetic characterization of bacterial consortia obtained of corroding gas pipelines in Mexico
journal, February 2008
- Jan-Roblero, Janet; Posadas, Adriana; Zavala Díaz de la Serna, Javier
- World Journal of Microbiology and Biotechnology, Vol. 24, Issue 9
UniFrac: a New Phylogenetic Method for Comparing Microbial Communities
journal, December 2005
- Lozupone, C.; Knight, R.
- Applied and Environmental Microbiology, Vol. 71, Issue 12
Haloanaerobium kushneri sp. nov., an obligately halophilic, anaerobic bacterium from an oil brine
journal, July 1999
- Bhupathiraju, V. K.; McInerney, M. J.; Woese, C. R.
- International Journal of Systematic Bacteriology, Vol. 49, Issue 3
Anaerobic bacteria from hypersaline environments
journal, March 1994
- Ollivier, B.; Caumette, P.; Garcia, J. L.
- Microbiological Reviews, Vol. 58, Issue 1
Genetic characterization of ompH mutants in the deep-sea bacterium Photobacterium sp. strain SS9
journal, December 1994
- Bartlett, D.; Chi, Ellen
- Archives of Microbiology, Vol. 162, Issue 5
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- Akyon, Benay; McLaughlin, Molly; Hernández, Felipe
- Environmental Science: Processes & Impacts, Vol. 21, Issue 2
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journal, June 2018
- Borton, Mikayla A.; Hoyt, David W.; Roux, Simon
- Proceedings of the National Academy of Sciences, Vol. 115, Issue 28
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journal, November 2018
- Evans, Morgan V.; Panescu, Jenny; Hanson, Andrea J.
- Frontiers in Microbiology, Vol. 9
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journal, December 2018
- Daly, Rebecca A.; Roux, Simon; Borton, Mikayla A.
- Nature Microbiology, Vol. 4, Issue 2
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journal, April 2018
- Lipus, Daniel; Roy, Dhritikshama; Khan, Eakalak
- FEMS Microbiology Letters, Vol. 365, Issue 12
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journal, June 2019
- Evans, Morgan V.; Getzinger, Gordon; Luek, Jenna L.
- The ISME Journal, Vol. 13, Issue 11