Multifunctional Cellulolytic Enzymes Outperform Processive Fungal Cellulases for Coproduction of Nanocellulose and Biofuels

Yarbrough, John. M.; Zhang, Ruoran; Mittal, Ashutosh; Vander Wall, Todd; Bomble, Yannick J.; Decker, Stephen R.; Himmel, Michael E.; Ciesielski, Peter N.

doi:10.1021/acsnano.7b00086

Title: Multifunctional Cellulolytic Enzymes Outperform Processive Fungal Cellulases for Coproduction of Nanocellulose and Biofuels

Journal Article · 06 March 2017 · ACS Nano

DOI: https://doi.org/10.1021/acsnano.7b00086 · OSTI ID:1351156

Yarbrough, John. M. ^[1]; Zhang, Ruoran ^[1]; Mittal, Ashutosh ^[1]; Vander Wall, Todd ^[1]; Bomble, Yannick J. ^[1]; Decker, Stephen R. ^[1]; Himmel, Michael E. ^[1];

^[1]

National Renewable Energy Lab. (NREL), Golden, CO (United States). Biosciences Center

Producing fuels, chemicals, and materials from renewable resources to meet societal demands remains an important step in the transition to a sustainable, clean energy economy. The use of cellulolytic enzymes for the production of nanocellulose enables the coproduction of sugars for biofuels production in a format that is largely compatible with the process design employed by modern lignocellulosic (second generation) biorefineries. However, yields of enzymatically produced nanocellulose are typically much lower than those achieved by mineral acid production methods. In this study, we compare the capacity for coproduction of nanocellulose and fermentable sugars using two vastly different cellulase systems: the classical 'free enzyme' system of the saprophytic fungus, Trichoderma reesei (T. reesei) and the complexed, multifunctional enzymes produced by the hot springs resident, Caldicellulosiruptor bescii (C. bescii). Here, we demonstrate by comparative digestions that the C. bescii system outperforms the fungal enzyme system in terms of total cellulose conversion, sugar production, and nanocellulose production. In addition, we show by multimodal imaging and dynamic light scattering that the nanocellulose produced by the C. bescii cellulase system is substantially more uniform than that produced by the T. reesei system. These disparities in the yields and characteristics of the nanocellulose produced by these disparate systems can be attributed to the dramatic differences in the mechanisms of action of the dominant enzymes in each system.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Laboratory Directed Research and Development (LDRD) Program; USDOE Office of Energy Efficiency and Renewable Energy (EERE)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1351156

Report Number(s):: NREL/JA--2700-68125

Journal Information:: ACS Nano, Journal Name: ACS Nano Journal Issue: 3 Vol. 11; ISSN 1936-0851

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Related Subjects

09 BIOMASS FUELS
nanocellulose
biofuel
enzymatic hydrolysis
cellulase
multi-functional enzyme

Title: Multifunctional Cellulolytic Enzymes Outperform Processive Fungal Cellulases for Coproduction of Nanocellulose and Biofuels

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