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Title: Enhanced agarose and xylan degradation for production of polyhydroxyalkanoates by co-culture of marine bacterium, Saccharophagus degradans and its contaminant, Bacillus cereus

Over reliance on energy or petroleum products has raised concerns both in regards to the depletion of their associated natural resources as well as their increasing costs. Bioplastics derived from microbes are emerging as promising alternatives to fossil fuel derived petroleum plastics. The development of a simple and eco-friendly strategy for bioplastic production with high productivity and yield, which is produced in a cost effective manner utilising abundantly available renewable carbon sources, would have the potential to result in an inexhaustible global energy source. Here we report the biosynthesis of bioplastic polyhydroxyalkanoates (PHAs) in pure cultures of marine bacterium, Saccharophagus degradans 2-40 ( Sde 2-40), its contaminant, Bacillus cereus, and a co-culture of these bacteria ( Sde 2-40 and B. cereus) degrading plant and algae derived complex polysaccharides. Sde 2-40 degraded the complex polysaccharides agarose and xylan as sole carbon sources for biosynthesis of PHAs. The ability of Sde 2-40 to degrade agarose increased after co-culturing with B. cereus. The association of Sde 2-40 with B. cereus resulted in increased cell growth and higher PHA production (34.5% of dry cell weight) from xylan as a carbon source in comparison to Sde 2-40 alone (22.7% of dry cell weight). Lastly, themore » present study offers an innovative prototype for production of PHA through consolidated bioprocessing of complex carbon sources by pure and co-culture of microorganisms.« less
 [1] ;  [1] ;  [2] ;  [1]
  1. Chungbuk National Univ., Chungbuk (Korea)
  2. National Renewable Energy Lab. (NREL), Golden, CO (United States)
Publication Date:
Report Number(s):
Journal ID: ISSN 2076-3417; ASPCC7
Grant/Contract Number:
Accepted Manuscript
Journal Name:
Applied Sciences
Additional Journal Information:
Journal Volume: 7; Journal Issue: 3; Journal ID: ISSN 2076-3417
Research Org:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
Country of Publication:
United States
09 BIOMASS FUELS; polyhydroxyalkanoates; agarose; xylan; Saccharophagus degradans; Bacillus cereus; consolidated bioprocessing
OSTI Identifier: