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Title: Enhanced agarose and xylan degradation for production of polyhydroxyalkanoates by co-culture of marine bacterium, Saccharophagus degradans and its contaminant, Bacillus cereus

Abstract

Over reliance on energy or petroleum products has raised concerns both in regards to the depletion of their associated natural resources as well as their increasing costs. Bioplastics derived from microbes are emerging as promising alternatives to fossil fuel derived petroleum plastics. The development of a simple and eco-friendly strategy for bioplastic production with high productivity and yield, which is produced in a cost effective manner utilising abundantly available renewable carbon sources, would have the potential to result in an inexhaustible global energy source. Here we report the biosynthesis of bioplastic polyhydroxyalkanoates (PHAs) in pure cultures of marine bacterium, Saccharophagus degradans 2-40 ( Sde 2-40), its contaminant, Bacillus cereus, and a co-culture of these bacteria ( Sde 2-40 and B. cereus) degrading plant and algae derived complex polysaccharides. Sde 2-40 degraded the complex polysaccharides agarose and xylan as sole carbon sources for biosynthesis of PHAs. The ability of Sde 2-40 to degrade agarose increased after co-culturing with B. cereus. The association of Sde 2-40 with B. cereus resulted in increased cell growth and higher PHA production (34.5% of dry cell weight) from xylan as a carbon source in comparison to Sde 2-40 alone (22.7% of dry cell weight). Lastly, themore » present study offers an innovative prototype for production of PHA through consolidated bioprocessing of complex carbon sources by pure and co-culture of microorganisms.« less

Authors:
 [1];  [1];  [2];  [1]
  1. Chungbuk National Univ., Chungbuk (Korea)
  2. National Renewable Energy Lab. (NREL), Golden, CO (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1350014
Report Number(s):
NREL/JA-2700-68266
Journal ID: ISSN 2076-3417; ASPCC7
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Accepted Manuscript
Journal Name:
Applied Sciences
Additional Journal Information:
Journal Volume: 7; Journal Issue: 3; Journal ID: ISSN 2076-3417
Publisher:
MDPI
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; polyhydroxyalkanoates; agarose; xylan; Saccharophagus degradans; Bacillus cereus; consolidated bioprocessing

Citation Formats

Sawant, Shailesh S., Salunke, Bipinchandra K., Taylor, II, Larry E., and Kim, Beom Soo. Enhanced agarose and xylan degradation for production of polyhydroxyalkanoates by co-culture of marine bacterium, Saccharophagus degradans and its contaminant, Bacillus cereus. United States: N. p., 2017. Web. doi:10.3390/app7030225.
Sawant, Shailesh S., Salunke, Bipinchandra K., Taylor, II, Larry E., & Kim, Beom Soo. Enhanced agarose and xylan degradation for production of polyhydroxyalkanoates by co-culture of marine bacterium, Saccharophagus degradans and its contaminant, Bacillus cereus. United States. doi:10.3390/app7030225.
Sawant, Shailesh S., Salunke, Bipinchandra K., Taylor, II, Larry E., and Kim, Beom Soo. Tue . "Enhanced agarose and xylan degradation for production of polyhydroxyalkanoates by co-culture of marine bacterium, Saccharophagus degradans and its contaminant, Bacillus cereus". United States. doi:10.3390/app7030225. https://www.osti.gov/servlets/purl/1350014.
@article{osti_1350014,
title = {Enhanced agarose and xylan degradation for production of polyhydroxyalkanoates by co-culture of marine bacterium, Saccharophagus degradans and its contaminant, Bacillus cereus},
author = {Sawant, Shailesh S. and Salunke, Bipinchandra K. and Taylor, II, Larry E. and Kim, Beom Soo},
abstractNote = {Over reliance on energy or petroleum products has raised concerns both in regards to the depletion of their associated natural resources as well as their increasing costs. Bioplastics derived from microbes are emerging as promising alternatives to fossil fuel derived petroleum plastics. The development of a simple and eco-friendly strategy for bioplastic production with high productivity and yield, which is produced in a cost effective manner utilising abundantly available renewable carbon sources, would have the potential to result in an inexhaustible global energy source. Here we report the biosynthesis of bioplastic polyhydroxyalkanoates (PHAs) in pure cultures of marine bacterium, Saccharophagus degradans 2-40 (Sde 2-40), its contaminant, Bacillus cereus, and a co-culture of these bacteria (Sde 2-40 and B. cereus) degrading plant and algae derived complex polysaccharides. Sde 2-40 degraded the complex polysaccharides agarose and xylan as sole carbon sources for biosynthesis of PHAs. The ability of Sde 2-40 to degrade agarose increased after co-culturing with B. cereus. The association of Sde 2-40 with B. cereus resulted in increased cell growth and higher PHA production (34.5% of dry cell weight) from xylan as a carbon source in comparison to Sde 2-40 alone (22.7% of dry cell weight). Lastly, the present study offers an innovative prototype for production of PHA through consolidated bioprocessing of complex carbon sources by pure and co-culture of microorganisms.},
doi = {10.3390/app7030225},
journal = {Applied Sciences},
number = 3,
volume = 7,
place = {United States},
year = {2017},
month = {2}
}

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    Works referencing / citing this record:

    Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production
    journal, June 2017


    Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production
    journal, June 2017