skip to main content

DOE PAGESDOE PAGES

Title: Acoustic injectors for drop-on-demand serial femtosecond crystallography

X-ray free-electron lasers (XFELs) provide very intense X-ray pulses suitable for macromolecular crystallography. Each X-ray pulse typically lasts for tens of femtoseconds and the interval between pulses is many orders of magnitude longer. Here we describe two novel acoustic injection systems that use focused sound waves to eject picoliter to nanoliter crystal-containing droplets out of microplates and into the X-ray pulse from which diffraction data are collected. The on-demand droplet delivery is synchronized to the XFEL pulse scheme, resulting in X-ray pulses intersecting up to 88% of the droplets. We tested several types of samples in a range of crystallization conditions, wherein the overall crystal hit ratio (e.g., fraction of images with observable diffraction patterns) is a function of the microcrystal slurry concentration. We report crystal structures from lysozyme, thermolysin, and stachydrine demethylase (Stc2). In addition, samples were screened to demonstrate that these methods can be applied to rare samples
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [5] ;  [6] ;  [7] ;  [8] ;  [4] ;  [8] ;  [9] ;  [4] ;  [5] ;  [10] ;  [11] ;  [4] ;  [10] ;  [10] ;  [4] ;  [10] more »;  [4] ;  [8] ;  [12] ;  [13] ;  [5] ;  [14] ;  [15] ;  [5] ;  [16] ;  [9] ;  [10] ;  [10] ;  [5] ;  [4] ;  [10] ;  [17] ;  [8] ;  [5] ;  [4] ;  [9] ;  [10] ;  [8] ;  [18] ;  [8] ;  [19] ;  [8] ;  [8] ;  [20] ;  [4] ;  [7] « less
  1. Brookhaven National Lab. (BNL), Upton, NY (United States); Ventana Medical Systems, Inc., Oro Valley, AZ (United States)
  2. Brookhaven National Lab. (BNL), Upton, NY (United States); St. Joseph's College, Patchogue, NY (United States)
  3. Brookhaven National Lab. (BNL), Upton, NY (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  4. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  5. Brookhaven National Lab. (BNL), Upton, NY (United States)
  6. Univ. de Sao Paulo, Sao Carlos (Brazil)
  7. Humboldt-Univ. zu Berlin, Berlin (Germany)
  8. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  9. Boston Univ., Boston, MA (United States)
  10. Labcyte Inc., Sunnyvale, CA (United States)
  11. Univ. of Central Florida, Orlando, FL (United States)
  12. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Howard Hughes Medical Institute, Ashburn, VA (United States)
  13. Technische Univ., Berlin (Germany); Humboldt-Univ. zu Berlin, Berlin (Gemany)
  14. Humboldt-Univ. zu Berlin, Berlin (Gemany)
  15. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); SLAC National Accelerator Lab., Menlo Park, CA (United States)
  16. SLAC National Accelerator Lab., Menlo Park, CA (United States); Paul Scherrer Institut, Villigen PSI (Switzerland)
  17. Brookhaven National Lab. (BNL), Upton, NY (United States); Diamond Light Source Ltd., Oxfordshire (United Kingdom)
  18. SLAC National Accelerator Lab., Menlo Park, CA (United States); Stanford Univ., Stanford, CA (United States)
  19. Univ. of Minnesota, Minneapolis, MN (United States)
  20. Univ. of Minnesota, Minneapolis, MN (United States); New Mexico State Univ., Las Cruces, NM (United States)
Publication Date:
Report Number(s):
BNL-112770-2016-JA
Journal ID: ISSN 0969-2126; PII: S0969212616000526
Grant/Contract Number:
AC02-76SF00515; 11-008; FWP BO-70; BO-9734; AC02-05CH11231; AC02-98CH10886; SC00112704
Type:
Published Article
Journal Name:
Structure
Additional Journal Information:
Journal Volume: 24; Journal Issue: 4; Journal ID: ISSN 0969-2126
Publisher:
Elsevier
Research Org:
Diamond Light Source Ltd., Oxfordshire (United Kingdom); Brookhaven National Laboratory (BNL), Upton, NY (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
OSTI Identifier:
1349763
Alternate Identifier(s):
OSTI ID: 1253633; OSTI ID: 1337424; OSTI ID: 1354254; OSTI ID: 1379277

Roessler, Christian  G., Agarwal, Rakhi, Allaire, Marc, Alonso-Mori, Roberto, Andi, Babak, Bachega, Jose  F. R., Bommer, Martin, Brewster, Aaron S., Browne, Michael  C., Chatterjee, Ruchira, Cho, Eunsun, Cohen, Aina  E., Cowan, Matthew, Datwani, Sammy, Davidson, Victor L., Defever, Jim, Eaton, Brent, Ellson, Richard, Feng, Yiping, Ghislain, Lucien P., Glownia, James  M., Han, Guangye, Hattne, Johan, Hellmich, Julia, Heroux, Annie, Ibrahim, Mohamed, Kern, Jan, Kuczewski, Anthony, Lemke, Henrik  T., Liu, Pinghua, Majlof, Lars, McClintock, William M., Myers, Stuart, Nelsen, Silke, Olechno, Joe, Orville, Allen M., Sauter, Nicholas K., Soares, Alexei S., Soltis, S. Michael, Song, Heng, Stearns, Richard G., Tran, Rosalie, Tsai, Yingssu, Uervirojnangkoorn, Monarin, Wilmot, Carrie M., Yachandra, Vittal, Yano, Junko, Yukl, Erik T., Zhu, Diling, and Zouni, Athina. Acoustic injectors for drop-on-demand serial femtosecond crystallography. United States: N. p., Web. doi:10.1016/j.str.2016.02.007.
Roessler, Christian  G., Agarwal, Rakhi, Allaire, Marc, Alonso-Mori, Roberto, Andi, Babak, Bachega, Jose  F. R., Bommer, Martin, Brewster, Aaron S., Browne, Michael  C., Chatterjee, Ruchira, Cho, Eunsun, Cohen, Aina  E., Cowan, Matthew, Datwani, Sammy, Davidson, Victor L., Defever, Jim, Eaton, Brent, Ellson, Richard, Feng, Yiping, Ghislain, Lucien P., Glownia, James  M., Han, Guangye, Hattne, Johan, Hellmich, Julia, Heroux, Annie, Ibrahim, Mohamed, Kern, Jan, Kuczewski, Anthony, Lemke, Henrik  T., Liu, Pinghua, Majlof, Lars, McClintock, William M., Myers, Stuart, Nelsen, Silke, Olechno, Joe, Orville, Allen M., Sauter, Nicholas K., Soares, Alexei S., Soltis, S. Michael, Song, Heng, Stearns, Richard G., Tran, Rosalie, Tsai, Yingssu, Uervirojnangkoorn, Monarin, Wilmot, Carrie M., Yachandra, Vittal, Yano, Junko, Yukl, Erik T., Zhu, Diling, & Zouni, Athina. Acoustic injectors for drop-on-demand serial femtosecond crystallography. United States. doi:10.1016/j.str.2016.02.007.
Roessler, Christian  G., Agarwal, Rakhi, Allaire, Marc, Alonso-Mori, Roberto, Andi, Babak, Bachega, Jose  F. R., Bommer, Martin, Brewster, Aaron S., Browne, Michael  C., Chatterjee, Ruchira, Cho, Eunsun, Cohen, Aina  E., Cowan, Matthew, Datwani, Sammy, Davidson, Victor L., Defever, Jim, Eaton, Brent, Ellson, Richard, Feng, Yiping, Ghislain, Lucien P., Glownia, James  M., Han, Guangye, Hattne, Johan, Hellmich, Julia, Heroux, Annie, Ibrahim, Mohamed, Kern, Jan, Kuczewski, Anthony, Lemke, Henrik  T., Liu, Pinghua, Majlof, Lars, McClintock, William M., Myers, Stuart, Nelsen, Silke, Olechno, Joe, Orville, Allen M., Sauter, Nicholas K., Soares, Alexei S., Soltis, S. Michael, Song, Heng, Stearns, Richard G., Tran, Rosalie, Tsai, Yingssu, Uervirojnangkoorn, Monarin, Wilmot, Carrie M., Yachandra, Vittal, Yano, Junko, Yukl, Erik T., Zhu, Diling, and Zouni, Athina. 2016. "Acoustic injectors for drop-on-demand serial femtosecond crystallography". United States. doi:10.1016/j.str.2016.02.007.
@article{osti_1349763,
title = {Acoustic injectors for drop-on-demand serial femtosecond crystallography},
author = {Roessler, Christian  G. and Agarwal, Rakhi and Allaire, Marc and Alonso-Mori, Roberto and Andi, Babak and Bachega, Jose  F. R. and Bommer, Martin and Brewster, Aaron S. and Browne, Michael  C. and Chatterjee, Ruchira and Cho, Eunsun and Cohen, Aina  E. and Cowan, Matthew and Datwani, Sammy and Davidson, Victor L. and Defever, Jim and Eaton, Brent and Ellson, Richard and Feng, Yiping and Ghislain, Lucien P. and Glownia, James  M. and Han, Guangye and Hattne, Johan and Hellmich, Julia and Heroux, Annie and Ibrahim, Mohamed and Kern, Jan and Kuczewski, Anthony and Lemke, Henrik  T. and Liu, Pinghua and Majlof, Lars and McClintock, William M. and Myers, Stuart and Nelsen, Silke and Olechno, Joe and Orville, Allen M. and Sauter, Nicholas K. and Soares, Alexei S. and Soltis, S. Michael and Song, Heng and Stearns, Richard G. and Tran, Rosalie and Tsai, Yingssu and Uervirojnangkoorn, Monarin and Wilmot, Carrie M. and Yachandra, Vittal and Yano, Junko and Yukl, Erik T. and Zhu, Diling and Zouni, Athina},
abstractNote = {X-ray free-electron lasers (XFELs) provide very intense X-ray pulses suitable for macromolecular crystallography. Each X-ray pulse typically lasts for tens of femtoseconds and the interval between pulses is many orders of magnitude longer. Here we describe two novel acoustic injection systems that use focused sound waves to eject picoliter to nanoliter crystal-containing droplets out of microplates and into the X-ray pulse from which diffraction data are collected. The on-demand droplet delivery is synchronized to the XFEL pulse scheme, resulting in X-ray pulses intersecting up to 88% of the droplets. We tested several types of samples in a range of crystallization conditions, wherein the overall crystal hit ratio (e.g., fraction of images with observable diffraction patterns) is a function of the microcrystal slurry concentration. We report crystal structures from lysozyme, thermolysin, and stachydrine demethylase (Stc2). In addition, samples were screened to demonstrate that these methods can be applied to rare samples},
doi = {10.1016/j.str.2016.02.007},
journal = {Structure},
number = 4,
volume = 24,
place = {United States},
year = {2016},
month = {3}
}