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Title: Micro-differential scanning calorimeter for liquid biological samples

Here, we developed an ultrasensitive micro-DSC (differential scanning calorimeter) for liquid protein sample characterization. Our design integrated vanadium oxide thermistors and flexible polymer substrates with microfluidics chambers to achieve a high sensitivity (6 V/W), low thermal conductivity (0.7 mW/K), high power resolutions (40 nW), and well-defined liquid volume (1 μl) calorimeter sensor in a compact and cost-effective way. Furthermore, we demonstrated the performance of the sensor with lysozyme unfolding. The measured transition temperature and enthalpy change were in accordance with the previous literature data. This micro-DSC could potentially raise the prospect of high-throughput biochemical measurement by parallel operation with miniaturized sample consumption.
ORCiD logo [1] ;  [2] ;  [3] ; ORCiD logo [4] ;  [4] ;  [5] ;  [2]
  1. Stony Brook Univ., NY (United States). Dept. of Mechanical Engineering
  2. Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Dept. of Mechanical Engineering
  3. AbbVie, Deutschland (Germany)
  4. AbbVie Bioresearch Center, Worcester, MA (United States)
  5. Brookhaven National Lab. (BNL), Upton, NY (United States). Center for Functional Nanomaterials
Publication Date:
Report Number(s):
Journal ID: ISSN 0034-6748; KC0403020; TRN: US1701353
Grant/Contract Number:
SC00112704; AC02-98CH10886
Accepted Manuscript
Journal Name:
Review of Scientific Instruments
Additional Journal Information:
Journal Volume: 87; Journal Issue: 10; Journal ID: ISSN 0034-6748
American Institute of Physics (AIP)
Research Org:
Brookhaven National Lab. (BNL), Upton, NY (United States). Center for Functional Nanomaterials (CFN)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
47 OTHER INSTRUMENTATION; 42 ENGINEERING; 59 BASIC BIOLOGICAL SCIENCES; Thermistors; BioMEMS; Microfluidics; Proteins; Polymers; Center for Functional Nanomaterials
OSTI Identifier: