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Title: Species differences in unlocking B-side electron transfer in bacterial reaction centers

The structure of the bacterial photosynthetic reaction center (RC) reveals symmetry-related electron transfer (ET) pathways, but only one path is used in native RCs. Analogous mutations have been made in two Rhodobacter (R.) species. A glutamic acid at position 133 in the M subunit increases transmembrane charge separation via the naturally inactive (B-side) path through impacts on primary ET in mutant R. sphaeroidesRCs. Prior work showed that the analogous substitution in the R. capsulatusRC also increases B-side activity, but mainly affects secondary ET. Finally, the overall yields of transmembrane ET are similar, but enabled in fundamentally different ways.
Authors:
 [1] ;  [2] ;  [1] ;  [1] ;  [1] ;  [2] ;  [2] ;  [1]
  1. Argonne National Lab. (ANL), Argonne, IL (United States). Biosciences Division
  2. Washington Univ., St. Louis, MO (United States). Dept. of Chemistry
Publication Date:
Grant/Contract Number:
AC02-06CH11357; SC0002036; DGE-1143954
Type:
Accepted Manuscript
Journal Name:
FEBS Letters
Additional Journal Information:
Journal Volume: 590; Journal Issue: 16; Journal ID: ISSN 0014-5793
Publisher:
Federation of European Biochemical Societies
Research Org:
Washington Univ., St. Louis, MO (United States); Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE Office of Science (SC), Workforce Development for Teachers and Scientists (WDTS) (SC-27); National Science Foundation (NSF)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; charge separation; high-throughput mutagenesis; photosynthetic bacteria; rapid screening; time-resolved spectroscopy
OSTI Identifier:
1349060
Alternate Identifier(s):
OSTI ID: 1400564