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Title: An early secretory pathway mediated by GNOM-LIKE 1 and GNOM is essential for basal polarity establishment in Arabidopsis thaliana

Abstract

Spatial regulation of the plant hormone indole-3-acetic acid (IAA, or auxin) is essential for plant development. Auxin gradient establishment is mediated by polarly localized auxin transporters, including PIN-FORMED (PIN) proteins. Their localization and abundance at the plasma membrane are tightly regulated by endomembrane machinery, especially the endocytic and recycling pathways mediated by the ADP ribosylation factor guanine nucleotide exchange factor (ARF-GEF) GNOM. We assessed the role of the early secretory pathway in establishing PIN1 polarity in Arabidopsis thaliana by pharmacological and genetic approaches. We identified the compound endosidin 8 (ES8), which selectively interferes with PIN1 basal polarity without altering the polarity of apical proteins. ES8 alters the auxin distribution pattern in the root and induces a strong developmental phenotype, including reduced root length. The ARF-GEF–defective mutants gnom-like 1 ( gnl1-1) and gnom ( van7) are significantly resistant to ES8. The compound does not affect recycling or vacuolar trafficking of PIN1 but leads to its intracellular accumulation, resulting in loss of PIN1 basal polarity at the plasma membrane. In conclusion, our data confirm a role for GNOM in endoplasmic reticulum (ER)–Golgi trafficking and reveal that a GNL1/GNOM-mediated early secretory pathway selectively regulates PIN1 basal polarity establishment in a manner essential formore » normal plant development.« less

Authors:
ORCiD logo [1];  [1];  [1];  [1];  [2];  [1];  [1];  [3];  [4];  [4];  [1]
  1. Swedish Univ. of Agricultural Sciences (Sweden)
  2. Umea Univ. (Sweden); Univ. of Potsdam (Germany)
  3. Ghent Univ. (Belgium); Institute of Science and Technology Austria (Austria)
  4. Univ. of California, Riverside, CA (United States)
Publication Date:
Research Org.:
Univ. of California, Riverside, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1348921
Grant/Contract Number:  
FG02-02ER15295; MCB-0817916; ERC-2011-StG-20101109-PSDP
Resource Type:
Accepted Manuscript
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Volume: 112; Journal Issue: 7; Journal ID: ISSN 0027-8424
Publisher:
National Academy of Sciences, Washington, DC (United States)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; chemical genomics; endomembrane trafficking; PIN auxin transporters; plasma membrane protein polarity

Citation Formats

Doyle, Siamsa M., Haeger, Ash, Vain, Thomas, Rigal, Adeline, Viotti, Corrado, Łangowska, Małgorzata, Ma, Qian, Friml, Jiří, Raikhel, Natasha V., Hicks, Glenn R., and Robert, Stéphanie. An early secretory pathway mediated by GNOM-LIKE 1 and GNOM is essential for basal polarity establishment in Arabidopsis thaliana. United States: N. p., 2015. Web. doi:10.1073/pnas.1424856112.
Doyle, Siamsa M., Haeger, Ash, Vain, Thomas, Rigal, Adeline, Viotti, Corrado, Łangowska, Małgorzata, Ma, Qian, Friml, Jiří, Raikhel, Natasha V., Hicks, Glenn R., & Robert, Stéphanie. An early secretory pathway mediated by GNOM-LIKE 1 and GNOM is essential for basal polarity establishment in Arabidopsis thaliana. United States. doi:10.1073/pnas.1424856112.
Doyle, Siamsa M., Haeger, Ash, Vain, Thomas, Rigal, Adeline, Viotti, Corrado, Łangowska, Małgorzata, Ma, Qian, Friml, Jiří, Raikhel, Natasha V., Hicks, Glenn R., and Robert, Stéphanie. Mon . "An early secretory pathway mediated by GNOM-LIKE 1 and GNOM is essential for basal polarity establishment in Arabidopsis thaliana". United States. doi:10.1073/pnas.1424856112. https://www.osti.gov/servlets/purl/1348921.
@article{osti_1348921,
title = {An early secretory pathway mediated by GNOM-LIKE 1 and GNOM is essential for basal polarity establishment in Arabidopsis thaliana},
author = {Doyle, Siamsa M. and Haeger, Ash and Vain, Thomas and Rigal, Adeline and Viotti, Corrado and Łangowska, Małgorzata and Ma, Qian and Friml, Jiří and Raikhel, Natasha V. and Hicks, Glenn R. and Robert, Stéphanie},
abstractNote = {Spatial regulation of the plant hormone indole-3-acetic acid (IAA, or auxin) is essential for plant development. Auxin gradient establishment is mediated by polarly localized auxin transporters, including PIN-FORMED (PIN) proteins. Their localization and abundance at the plasma membrane are tightly regulated by endomembrane machinery, especially the endocytic and recycling pathways mediated by the ADP ribosylation factor guanine nucleotide exchange factor (ARF-GEF) GNOM. We assessed the role of the early secretory pathway in establishing PIN1 polarity in Arabidopsis thaliana by pharmacological and genetic approaches. We identified the compound endosidin 8 (ES8), which selectively interferes with PIN1 basal polarity without altering the polarity of apical proteins. ES8 alters the auxin distribution pattern in the root and induces a strong developmental phenotype, including reduced root length. The ARF-GEF–defective mutants gnom-like 1 (gnl1-1) and gnom (van7) are significantly resistant to ES8. The compound does not affect recycling or vacuolar trafficking of PIN1 but leads to its intracellular accumulation, resulting in loss of PIN1 basal polarity at the plasma membrane. In conclusion, our data confirm a role for GNOM in endoplasmic reticulum (ER)–Golgi trafficking and reveal that a GNL1/GNOM-mediated early secretory pathway selectively regulates PIN1 basal polarity establishment in a manner essential for normal plant development.},
doi = {10.1073/pnas.1424856112},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 7,
volume = 112,
place = {United States},
year = {2015},
month = {2}
}

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