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Title: Lipid and glycolipid isomer analyses using ultra-high resolution ion mobility spectrometry separations

Understanding the biological mechanisms related to lipids and glycolipids is challenging due to the vast number of possible isomers. Mass spectrometry (MS) measurements are currently the dominant approach for studying and providing detailed information on lipid and glycolipid structures. However, difficulties in distinguishing many structural isomers (e.g. distinct acyl chain positions, double bond locations, as well as glycan isomers) inhibit the understanding of their biological roles. Here we utilized ultra-high resolution ion mobility spectrometry (IMS) separations based upon the use of traveling waves in a serpentine long path length multi-pass Structures for Lossless Manipulations (SLIM) to enhance isomer resolution. The multi-pass arrangement allowed separations ranging from ~16 m (1 pass) to ~470 m (32 passes) to be investigated for the distinction of lipids and glycolipids with extremely small structural differences. Lastly, these ultra-high resolution SLIM IMS-MS analyses provide a foundation for exploring and better understanding isomer specific biological and disease processes.
Authors:
 [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Publication Date:
Report Number(s):
PNNL-SA-122274
Journal ID: ISSN 1422-0067; IJMCFK; 48680; 48135; 453040220
Grant/Contract Number:
AC05-76RL01830
Type:
Accepted Manuscript
Journal Name:
International Journal of Molecular Sciences (Online)
Additional Journal Information:
Journal Name: International Journal of Molecular Sciences (Online); Journal Volume: 18; Journal Issue: 1; Journal ID: ISSN 1422-0067
Publisher:
MDPI
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Environmental Molecular Sciences Laboratory; lipids; glycolipids; isomers; ion mobility spectrometry
OSTI Identifier:
1347858

Wojcik, Roza, Webb, Ian K., Deng, Liulin, Garimella, Sandilya V. B., Prost, Spencer A., Ibrahim, Yehia M., Baker, Erin S., and Smith, Richard D.. Lipid and glycolipid isomer analyses using ultra-high resolution ion mobility spectrometry separations. United States: N. p., Web. doi:10.3390/ijms18010183.
Wojcik, Roza, Webb, Ian K., Deng, Liulin, Garimella, Sandilya V. B., Prost, Spencer A., Ibrahim, Yehia M., Baker, Erin S., & Smith, Richard D.. Lipid and glycolipid isomer analyses using ultra-high resolution ion mobility spectrometry separations. United States. doi:10.3390/ijms18010183.
Wojcik, Roza, Webb, Ian K., Deng, Liulin, Garimella, Sandilya V. B., Prost, Spencer A., Ibrahim, Yehia M., Baker, Erin S., and Smith, Richard D.. 2017. "Lipid and glycolipid isomer analyses using ultra-high resolution ion mobility spectrometry separations". United States. doi:10.3390/ijms18010183. https://www.osti.gov/servlets/purl/1347858.
@article{osti_1347858,
title = {Lipid and glycolipid isomer analyses using ultra-high resolution ion mobility spectrometry separations},
author = {Wojcik, Roza and Webb, Ian K. and Deng, Liulin and Garimella, Sandilya V. B. and Prost, Spencer A. and Ibrahim, Yehia M. and Baker, Erin S. and Smith, Richard D.},
abstractNote = {Understanding the biological mechanisms related to lipids and glycolipids is challenging due to the vast number of possible isomers. Mass spectrometry (MS) measurements are currently the dominant approach for studying and providing detailed information on lipid and glycolipid structures. However, difficulties in distinguishing many structural isomers (e.g. distinct acyl chain positions, double bond locations, as well as glycan isomers) inhibit the understanding of their biological roles. Here we utilized ultra-high resolution ion mobility spectrometry (IMS) separations based upon the use of traveling waves in a serpentine long path length multi-pass Structures for Lossless Manipulations (SLIM) to enhance isomer resolution. The multi-pass arrangement allowed separations ranging from ~16 m (1 pass) to ~470 m (32 passes) to be investigated for the distinction of lipids and glycolipids with extremely small structural differences. Lastly, these ultra-high resolution SLIM IMS-MS analyses provide a foundation for exploring and better understanding isomer specific biological and disease processes.},
doi = {10.3390/ijms18010183},
journal = {International Journal of Molecular Sciences (Online)},
number = 1,
volume = 18,
place = {United States},
year = {2017},
month = {1}
}