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Title: Biomarkers are used to predict quantitative metabolite concentration profiles in human red blood cells

Deep-coverage metabolomic profiling has revealed a well-defined development of metabolic decay in human red blood cells (RBCs) under cold storage conditions. A set of extracellular biomarkers has been recently identified that reliably defines the qualitative state of the metabolic network throughout this metabolic decay process. Here, we extend the utility of these biomarkers by using them to quantitatively predict the concentrations of other metabolites in the red blood cell. We are able to accurately predict the concentration profile of 84 of the 91 (92%) measured metabolites ( p < 0.05) in RBC metabolism using only measurements of these five biomarkers. The median of prediction errors (symmetric mean absolute percent error) across all metabolites was 13%. Furthermore, the ability to predict numerous metabolite concentrations from a simple set of biomarkers offers the potential for the development of a powerful workflow that could be used to evaluate the metabolic state of a biological system using a minimal set of measurements.
Authors:
ORCiD logo [1] ; ORCiD logo [1] ;  [1] ;  [2]
  1. Univ. of California, San Diego, La Jolla, CA (United States)
  2. Chalmers Univ. of Technology (Sweden)
Publication Date:
Grant/Contract Number:
SC0008701
Type:
Published Article
Journal Name:
PLoS Computational Biology (Online)
Additional Journal Information:
Journal Name: PLoS Computational Biology (Online); Journal Volume: 13; Journal Issue: 3; Journal ID: ISSN 1553-7358
Publisher:
Public Library of Science
Research Org:
Univ. of California, San Diego, CA (United States); Univ. of California at San Diego, La Jolla, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; storage; metabolomics; identification; discovery; unit
OSTI Identifier:
1347468
Alternate Identifier(s):
OSTI ID: 1345712; OSTI ID: 1355929; OSTI ID: 1366707