Enhanced delignification of steam-pretreated poplar by a bacterial laccase
Abstract
The recalcitrance of woody biomass, particularly its lignin component, hinders its sustainable transformation to fuels and biomaterials. Although the recent discovery of several bacterial ligninases promises the development of novel biocatalysts, these enzymes have largely been characterized using model substrates: direct evidence for their action on biomass is lacking. Herein, we report the delignification of woody biomass by a small laccase (sLac) from Amycolatopsis sp. 75iv3. Incubation of steam-pretreated poplar (SPP) with sLac enhanced the release of acid-precipitable polymeric lignin (APPL) by ~6-fold, and reduced the amount of acid-soluble lignin by ~15%. NMR spectrometry revealed that the APPL was significantly syringyl-enriched relative to the original material (~16:1 vs. ~3:1), and that sLac preferentially oxidized syringyl units and altered interunit linkage distributions. sLac’s substrate preference among monoaryls was also consistent with this observation. In addition, sLac treatment reduced the molar mass of the APPL by over 50%, as determined by gel-permeation chromatography coupled with multi-angle light scattering. Finally, sLac acted synergistically with a commercial cellulase cocktail to increase glucose production from SPP ~8%. Altogether, this study establishes the lignolytic activity of sLac on woody biomass and highlights the biocatalytic potential of bacterial enzymes.
- Authors:
-
- The Univ. of British Columbia, Vancouver, BC (Canada)
- Wisconsin Energy Institute, Madison, WI (United States); Univ. of Wisconsin, Madison, WI (United States)
- Publication Date:
- Research Org.:
- Univ. of Wisconsin, Madison, WI (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1347435
- Grant/Contract Number:
- FC02-07ER64494
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Scientific Reports
- Additional Journal Information:
- Journal Volume: 7; Journal ID: ISSN 2045-2322
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; chemical biology; environmental biotechnology
Citation Formats
Singh, Rahul, Hu, Jinguang, Regner, Matthew R., Round, James W., Ralph, John, Saddler, John N., and Eltis, Lindsay D. Enhanced delignification of steam-pretreated poplar by a bacterial laccase. United States: N. p., 2017.
Web. doi:10.1038/srep42121.
Singh, Rahul, Hu, Jinguang, Regner, Matthew R., Round, James W., Ralph, John, Saddler, John N., & Eltis, Lindsay D. Enhanced delignification of steam-pretreated poplar by a bacterial laccase. United States. https://doi.org/10.1038/srep42121
Singh, Rahul, Hu, Jinguang, Regner, Matthew R., Round, James W., Ralph, John, Saddler, John N., and Eltis, Lindsay D. Tue .
"Enhanced delignification of steam-pretreated poplar by a bacterial laccase". United States. https://doi.org/10.1038/srep42121. https://www.osti.gov/servlets/purl/1347435.
@article{osti_1347435,
title = {Enhanced delignification of steam-pretreated poplar by a bacterial laccase},
author = {Singh, Rahul and Hu, Jinguang and Regner, Matthew R. and Round, James W. and Ralph, John and Saddler, John N. and Eltis, Lindsay D.},
abstractNote = {The recalcitrance of woody biomass, particularly its lignin component, hinders its sustainable transformation to fuels and biomaterials. Although the recent discovery of several bacterial ligninases promises the development of novel biocatalysts, these enzymes have largely been characterized using model substrates: direct evidence for their action on biomass is lacking. Herein, we report the delignification of woody biomass by a small laccase (sLac) from Amycolatopsis sp. 75iv3. Incubation of steam-pretreated poplar (SPP) with sLac enhanced the release of acid-precipitable polymeric lignin (APPL) by ~6-fold, and reduced the amount of acid-soluble lignin by ~15%. NMR spectrometry revealed that the APPL was significantly syringyl-enriched relative to the original material (~16:1 vs. ~3:1), and that sLac preferentially oxidized syringyl units and altered interunit linkage distributions. sLac’s substrate preference among monoaryls was also consistent with this observation. In addition, sLac treatment reduced the molar mass of the APPL by over 50%, as determined by gel-permeation chromatography coupled with multi-angle light scattering. Finally, sLac acted synergistically with a commercial cellulase cocktail to increase glucose production from SPP ~8%. Altogether, this study establishes the lignolytic activity of sLac on woody biomass and highlights the biocatalytic potential of bacterial enzymes.},
doi = {10.1038/srep42121},
journal = {Scientific Reports},
number = ,
volume = 7,
place = {United States},
year = {Tue Feb 07 00:00:00 EST 2017},
month = {Tue Feb 07 00:00:00 EST 2017}
}
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