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Title: The MYB107 Transcription Factor Positively Regulates Suberin Biosynthesis

Suberin, a lipophilic polymer deposited in the outer integument of the Arabidopsis ( Arabidopsis thaliana) seed coat, represents an essential sealing component controlling water and solute movement and protecting seed from pathogenic infection. Although many genes responsible for suberin synthesis are identified, the regulatory components controlling its biosynthesis have not been definitively determined. Here, we show that the Arabidopsis MYB107 transcription factor acts as a positive regulator controlling suberin biosynthetic gene expression in the seed coat. MYB107 coexpresses with suberin biosynthetic genes in a temporal manner during seed development. Disrupting MYB107 particularly suppresses the expression of genes involved in suberin but not cutin biosynthesis, lowers seed coat suberin accumulation, alters suberin lamellar structure, and consequently renders higher seed coat permeability and susceptibility to abiotic stresses. Furthermore, MYB107 directly binds to the promoters of suberin biosynthetic genes, verifying its primary role in regulating their expression. Identifying MYB107 as a positive regulator for seed coat suberin synthesis offers a basis for discovering the potential transcriptional network behind one of the most abundant lipid-based polymers in nature.
Authors:
ORCiD logo [1] ; ORCiD logo [1] ;  [1] ; ORCiD logo [1] ;  [1] ;  [1] ; ORCiD logo [1]
  1. Brookhaven National Lab. (BNL), Upton, NY (United States). Biology Department; Appalachian State University, Boone, NC (United States)
Publication Date:
Report Number(s):
BNL-113545-2017-JA
Journal ID: ISSN 0032-0889
Grant/Contract Number:
SC00112704; AC02-98CH10886
Type:
Accepted Manuscript
Journal Name:
Plant Physiology (Bethesda)
Additional Journal Information:
Journal Name: Plant Physiology (Bethesda); Journal Volume: 173; Journal Issue: 2; Journal ID: ISSN 0032-0889
Publisher:
American Society of Plant Biologists
Research Org:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Transcription factor; MYB107; Suberin; Cutin; Seed coat; Lamellae
OSTI Identifier:
1347288

Gou, Mingyue, Hou, Guichuan, Yang, Huijun, Zhang, Xuebin, Cai, Yuanheng, Kai, Guoyin, and Liu, Chang-Jun. The MYB107 Transcription Factor Positively Regulates Suberin Biosynthesis. United States: N. p., Web. doi:10.1104/pp.16.01614.
Gou, Mingyue, Hou, Guichuan, Yang, Huijun, Zhang, Xuebin, Cai, Yuanheng, Kai, Guoyin, & Liu, Chang-Jun. The MYB107 Transcription Factor Positively Regulates Suberin Biosynthesis. United States. doi:10.1104/pp.16.01614.
Gou, Mingyue, Hou, Guichuan, Yang, Huijun, Zhang, Xuebin, Cai, Yuanheng, Kai, Guoyin, and Liu, Chang-Jun. 2016. "The MYB107 Transcription Factor Positively Regulates Suberin Biosynthesis". United States. doi:10.1104/pp.16.01614. https://www.osti.gov/servlets/purl/1347288.
@article{osti_1347288,
title = {The MYB107 Transcription Factor Positively Regulates Suberin Biosynthesis},
author = {Gou, Mingyue and Hou, Guichuan and Yang, Huijun and Zhang, Xuebin and Cai, Yuanheng and Kai, Guoyin and Liu, Chang-Jun},
abstractNote = {Suberin, a lipophilic polymer deposited in the outer integument of the Arabidopsis (Arabidopsis thaliana) seed coat, represents an essential sealing component controlling water and solute movement and protecting seed from pathogenic infection. Although many genes responsible for suberin synthesis are identified, the regulatory components controlling its biosynthesis have not been definitively determined. Here, we show that the Arabidopsis MYB107 transcription factor acts as a positive regulator controlling suberin biosynthetic gene expression in the seed coat. MYB107 coexpresses with suberin biosynthetic genes in a temporal manner during seed development. Disrupting MYB107 particularly suppresses the expression of genes involved in suberin but not cutin biosynthesis, lowers seed coat suberin accumulation, alters suberin lamellar structure, and consequently renders higher seed coat permeability and susceptibility to abiotic stresses. Furthermore, MYB107 directly binds to the promoters of suberin biosynthetic genes, verifying its primary role in regulating their expression. Identifying MYB107 as a positive regulator for seed coat suberin synthesis offers a basis for discovering the potential transcriptional network behind one of the most abundant lipid-based polymers in nature.},
doi = {10.1104/pp.16.01614},
journal = {Plant Physiology (Bethesda)},
number = 2,
volume = 173,
place = {United States},
year = {2016},
month = {12}
}