Structural analysis of Notch-regulating Rumi reveals basis for pathogenic mutations
Abstract
We present Rumi O-glucosylates the EGF repeats of a growing list of proteins essential in metazoan development, including Notch. Rumi is essential for Notch signaling, and Rumi dysregulation is linked to several human diseases. Despite Rumi's critical roles, it is unknown how Rumi glucosylates a serine of many but not all EGF repeats. Here we report crystal structures of Drosophila Rumi as binary and ternary complexes with a folded EGF repeat and/or donor substrates. These structures provide insights into the catalytic mechanism and show that Rumi recognizes structural signatures of the EGF motif, the U-shaped consensus sequence, C-X-S-X-(P/A)-C and a conserved hydrophobic region. We found that five Rumi mutations identified in cancers and Dowling–Degos disease are clustered around the enzyme active site and adversely affect its activity. In conclusion, our study suggests that loss of Rumi activity may underlie these diseases, and the mechanistic insights may facilitate the development of modulators of Notch signaling.
- Authors:
-
- Brookhaven National Lab. (BNL), Upton, NY (United States). Biology Department
- Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center
- Stony Brook Univ., NY (United States). Department of Biochemistry and Cell Biology
- Publication Date:
- Research Org.:
- Brookhaven National Laboratory (BNL), Upton, NY (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institute of Health
- OSTI Identifier:
- 1347286
- Report Number(s):
- BNL-113513-2017-JA
Journal ID: ISSN 1552-4450
- Grant/Contract Number:
- SC00112704; AC02-98CH10886; AC02-06CH11357
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Chemical Biology
- Additional Journal Information:
- Journal Volume: 12; Journal Issue: 9; Journal ID: ISSN 1552-4450
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; Cancer; Enzyme mechanisms; Glycobiology; X-ray crystallography
Citation Formats
Yu, Hongjun, Takeuchi, Hideyuki, Takeuchi, Megumi, Liu, Qun, Kantharia, Joshua, Haltiwanger, Robert S., and Li, Huilin. Structural analysis of Notch-regulating Rumi reveals basis for pathogenic mutations. United States: N. p., 2016.
Web. doi:10.1038/nchembio.2135.
Yu, Hongjun, Takeuchi, Hideyuki, Takeuchi, Megumi, Liu, Qun, Kantharia, Joshua, Haltiwanger, Robert S., & Li, Huilin. Structural analysis of Notch-regulating Rumi reveals basis for pathogenic mutations. United States. https://doi.org/10.1038/nchembio.2135
Yu, Hongjun, Takeuchi, Hideyuki, Takeuchi, Megumi, Liu, Qun, Kantharia, Joshua, Haltiwanger, Robert S., and Li, Huilin. Mon .
"Structural analysis of Notch-regulating Rumi reveals basis for pathogenic mutations". United States. https://doi.org/10.1038/nchembio.2135. https://www.osti.gov/servlets/purl/1347286.
@article{osti_1347286,
title = {Structural analysis of Notch-regulating Rumi reveals basis for pathogenic mutations},
author = {Yu, Hongjun and Takeuchi, Hideyuki and Takeuchi, Megumi and Liu, Qun and Kantharia, Joshua and Haltiwanger, Robert S. and Li, Huilin},
abstractNote = {We present Rumi O-glucosylates the EGF repeats of a growing list of proteins essential in metazoan development, including Notch. Rumi is essential for Notch signaling, and Rumi dysregulation is linked to several human diseases. Despite Rumi's critical roles, it is unknown how Rumi glucosylates a serine of many but not all EGF repeats. Here we report crystal structures of Drosophila Rumi as binary and ternary complexes with a folded EGF repeat and/or donor substrates. These structures provide insights into the catalytic mechanism and show that Rumi recognizes structural signatures of the EGF motif, the U-shaped consensus sequence, C-X-S-X-(P/A)-C and a conserved hydrophobic region. We found that five Rumi mutations identified in cancers and Dowling–Degos disease are clustered around the enzyme active site and adversely affect its activity. In conclusion, our study suggests that loss of Rumi activity may underlie these diseases, and the mechanistic insights may facilitate the development of modulators of Notch signaling.},
doi = {10.1038/nchembio.2135},
journal = {Nature Chemical Biology},
number = 9,
volume = 12,
place = {United States},
year = {Mon Jul 18 00:00:00 EDT 2016},
month = {Mon Jul 18 00:00:00 EDT 2016}
}
Web of Science
Works referenced in this record:
Structures from Anomalous Diffraction of Native Biological Macromolecules
journal, May 2012
- Liu, Q.; Dahmane, T.; Zhang, Z.
- Science, Vol. 336, Issue 6084
Overview of the CCP 4 suite and current developments
journal, March 2011
- Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D.
- Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4
Substructure solution with SHELXD
journal, September 2002
- Schneider, Thomas R.; Sheldrick, George M.
- Acta Crystallographica Section D Biological Crystallography, Vol. 58, Issue 10
Exome Sequencing of Head and Neck Squamous Cell Carcinoma Reveals Inactivating Mutations in NOTCH1
journal, July 2011
- Agrawal, N.; Frederick, M. J.; Pickering, C. R.
- Science, Vol. 333, Issue 6046
Rumi Is a CAP10 Domain Glycosyltransferase that Modifies Notch and Is Required for Notch Signaling
journal, January 2008
- Acar, Melih; Jafar-Nejad, Hamed; Takeuchi, Hideyuki
- Cell, Vol. 132, Issue 2
Dali server: conservation mapping in 3D
journal, May 2010
- Holm, Liisa; Rosenstr�m, P�ivi
- Nucleic Acids Research, Vol. 38, Issue suppl_2
Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism
journal, September 2015
- Yu, Hongjun; Takeuchi, Megumi; LeBarron, Jamie
- Nature Chemical Biology, Vol. 11, Issue 11
A new tumor suppressor role for the Notch pathway in bladder cancer
journal, September 2014
- Rampias, Theodoros; Vgenopoulou, Paraskevi; Avgeris, Margaritis
- Nature Medicine, Vol. 20, Issue 10
Rational targeting of Notch signaling in cancer
journal, September 2008
- Rizzo, P.; Osipo, C.; Foreman, K.
- Oncogene, Vol. 27, Issue 38
Protein O-Glucosyltransferase 1 (POGLUT1) Promotes Mouse Gastrulation through Modification of the Apical Polarity Protein CRUMBS2
journal, October 2015
- Ramkumar, Nitya; Harvey, Beth M.; Lee, Jeffrey D.
- PLOS Genetics, Vol. 11, Issue 10
Structural Insights into the Mechanism of Protein O-Fucosylation
journal, September 2011
- Lira-Navarrete, Erandi; Valero-González, Jessika; Villanueva, Raquel
- PLoS ONE, Vol. 6, Issue 9
Structure of human O-GlcNAc transferase and its complex with a peptide substrate
journal, January 2011
- Lazarus, Michael B.; Nam, Yunsun; Jiang, Jiaoyang
- Nature, Vol. 469, Issue 7331
A novel tumour-suppressor function for the Notch pathway in myeloid leukaemia
journal, May 2011
- Klinakis, Apostolos; Lobry, Camille; Abdel-Wahab, Omar
- Nature, Vol. 473, Issue 7346
Regions of Drosophila Notch That Contribute to Ligand Binding and the Modulatory Influence of Fringe
journal, June 2005
- Xu, Aiguo; Lei, Liang; Irvine, Kenneth D.
- Journal of Biological Chemistry, Vol. 280, Issue 34
Recent structures, evolution and mechanisms of glycosyltransferases
journal, October 2012
- Breton, Christelle; Fournel-Gigleux, Sylvie; Palcic, Monica M.
- Current Opinion in Structural Biology, Vol. 22, Issue 5
Fringe benefits: Functional and structural impacts of O-glycosylation on the extracellular domain of Notch receptors
journal, October 2011
- Rana, Nadia A.; Haltiwanger, Robert S.
- Current Opinion in Structural Biology, Vol. 21, Issue 5
Loss-of-function mutations in Notch receptors in cutaneous and lung squamous cell carcinoma
journal, October 2011
- Wang, N. J.; Sanborn, Z.; Arnett, K. L.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 43
Mutations in POGLUT1, Encoding Protein O-Glucosyltransferase 1, Cause Autosomal-Dominant Dowling-Degos Disease
journal, January 2014
- Basmanav, F. Buket; Oprisoreanu, Ana-Maria; Pasternack, Sandra M.
- The American Journal of Human Genetics, Vol. 94, Issue 1
Structure of human POFUT2: insights into thrombospondin type 1 repeat fold and O -fucosylation : Structure of human protein
journal, May 2012
- Chen, Chun-I; Keusch, Jeremy J.; Klein, Dominique
- The EMBO Journal, Vol. 31, Issue 14
Rumi functions as both a protein O-glucosyltransferase and a protein O-xylosyltransferase
journal, September 2011
- Takeuchi, H.; Fernandez-Valdivia, R. C.; Caswell, D. S.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 40
X-ray structure of a bacterial oligosaccharyltransferase
journal, June 2011
- Lizak, Christian; Gerber, Sabina; Numao, Shin
- Nature, Vol. 474, Issue 7351
XDS
journal, January 2010
- Kabsch, Wolfgang
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2
Regulation of mammalian Notch signaling and embryonic development by the protein O-glucosyltransferase Rumi
journal, April 2011
- Fernandez-Valdivia, R.; Takeuchi, H.; Samarghandi, A.
- Development, Vol. 138, Issue 10
Novel roles for O-linked glycans in protein folding
journal, September 2014
- Vasudevan, Deepika; Haltiwanger, Robert S.
- Glycoconjugate Journal, Vol. 31, Issue 6-7
Generalized Solar Lentigines in a Patient with a History of Radon Exposure
journal, January 2010
- Mauerer, Andreas; Betz, Regina C.; Pasternack, Sandra M.
- Dermatology, Vol. 221, Issue 3
Incorporation of Prior Phase Information Strengthens Maximum-Likelihood Structure Refinement
journal, November 1998
- Pannu, Navraj S.; Murshudov, Garib N.; Dodson, Eleanor J.
- Acta Crystallographica Section D Biological Crystallography, Vol. 54, Issue 6
The cBio Cancer Genomics Portal: An Open Platform for Exploring Multidimensional Cancer Genomics Data: Figure 1.
journal, May 2012
- Cerami, Ethan; Gao, Jianjiong; Dogrusoz, Ugur
- Cancer Discovery, Vol. 2, Issue 5
iMOSFLM : a new graphical interface for diffraction-image processing with MOSFLM
journal, March 2011
- Battye, T. Geoff G.; Kontogiannis, Luke; Johnson, Owen
- Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4
Site-specific O -Glucosylation of the Epidermal Growth Factor-like (EGF) Repeats of Notch : EFFICIENCY OF GLYCOSYLATION IS AFFECTED BY PROPER FOLDING AND AMINO ACID SEQUENCE OF INDIVIDUAL EGF REPEATS
journal, August 2012
- Takeuchi, Hideyuki; Kantharia, Joshua; Sethi, Maya K.
- Journal of Biological Chemistry, Vol. 287, Issue 41
Down-regulated expression of Notch signaling molecules in human endometrial cancer
journal, January 2013
- Jonusiene, Violeta; Sasnauskiene, Ausra; Lachej, Nadezda
- Medical Oncology, Vol. 30, Issue 1
The carbohydrate-active enzymes database (CAZy) in 2013
journal, November 2013
- Lombard, Vincent; Golaconda Ramulu, Hemalatha; Drula, Elodie
- Nucleic Acids Research, Vol. 42, Issue D1
Therapeutic modulation of Notch signalling — are we there yet?
journal, April 2014
- Andersson, Emma R.; Lendahl, Urban
- Nature Reviews Drug Discovery, Vol. 13, Issue 5
Features and development of Coot
journal, March 2010
- Emsley, P.; Lohkamp, B.; Scott, W. G.
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4
O-GlcNAc transferase invokes nucleotide sugar pyrophosphate participation in catalysis
journal, October 2012
- Schimpl, Marianne; Zheng, Xiaowei; Borodkin, Vladimir S.
- Nature Chemical Biology, Vol. 8, Issue 12
Structural snapshots of the reaction coordinate for O-GlcNAc transferase
journal, October 2012
- Lazarus, Michael B.; Jiang, Jiaoyang; Gloster, Tracey M.
- Nature Chemical Biology, Vol. 8, Issue 12
PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010
- Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221
Evolution of distinct EGF domains with specific functions
journal, April 2005
- Wouters, Merridee A.; Rigoutsos, Isidore; Chu, Carmen K.
- Protein Science, Vol. 14, Issue 4
Multiple O -glucosylation sites on Notch function as a buffer against temperature-dependent loss of signaling
journal, July 2011
- Leonardi, Jessica; Fernandez-Valdivia, Rodrigo; Li, Yi-Dong
- Development, Vol. 138, Issue 16
Structural Evidence of a Passive Base-flipping Mechanism for AGT, an Unusual GT-B Glycosyltransferase
journal, September 2005
- Larivière, Laurent; Sommer, Nicole; Moréra, Solange
- Journal of Molecular Biology, Vol. 352, Issue 1
Glycosyltransferases: Structures, Functions, and Mechanisms
journal, June 2008
- Lairson, L. L.; Henrissat, B.; Davies, G. J.
- Annual Review of Biochemistry, Vol. 77, Issue 1
A proactive role of water molecules in acceptor recognition by protein O-fucosyltransferase 2
journal, February 2016
- Valero-González, Jessika; Leonhard-Melief, Christina; Lira-Navarrete, Erandi
- Nature Chemical Biology, Vol. 12, Issue 4
Molecular replacement with MOLREP
journal, December 2009
- Vagin, Alexei; Teplyakov, Alexei
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 1
From Fly Wings to Targeted Cancer Therapies: A Centennial for Notch Signaling
journal, March 2014
- Ntziachristos, Panagiotis; Lim, Jing Shan; Sage, Julien
- Cancer Cell, Vol. 25, Issue 3
Morbus Galli-Galli: Klinische und histopathologische Untersuchung anhand einer Fallserie von 18 Patienten
journal, October 2011
- Hanneken, S.; Rütten, A.; Eigelshoven, S.
- Der Hautarzt, Vol. 62, Issue 11
[20] Processing of X-ray diffraction data collected in oscillation mode
book, January 1997
- Otwinowski, Zbyszek; Minor, Wladek
- Macromolecular Crystallography Part A, 307-326
Negative Regulation of Notch Signaling by Xylose
journal, June 2013
- Lee, Tom V.; Sethi, Maya K.; Leonardi, Jessica
- PLoS Genetics, Vol. 9, Issue 6
The Canonical Notch Signaling Pathway: Unfolding the Activation Mechanism
journal, April 2009
- Kopan, Raphael; Ilagan, Ma. Xenia G.
- Cell, Vol. 137, Issue 2
Jagged1 heterozygosity in mice results in a congenital cholangiopathy which is reversed by concomitant deletion of one copy of Poglut1 ( Rumi ): Thakurdas et al.
journal, October 2015
- Thakurdas, Shakeel M.; Lopez, Mario F.; Kakuda, Shinako
- Hepatology, Vol. 63, Issue 2
HCF-1 Is Cleaved in the Active Site of O-GlcNAc Transferase
journal, December 2013
- Lazarus, M. B.; Jiang, J.; Kapuria, V.
- Science, Vol. 342, Issue 6163
The Protein O-glucosyltransferase Rumi Modifies Eyes Shut to Promote Rhabdomere Separation in Drosophila
journal, November 2014
- Haltom, Amanda R.; Lee, Tom V.; Harvey, Beth M.
- PLoS Genetics, Vol. 10, Issue 11
PARP1 exhibits enhanced association and catalytic efficiency with γH2A.X-nucleosome
journal, December 2019
- Sharma, Deepti; De Falco, Louis; Padavattan, Sivaraman
- Nature Communications, Vol. 10, Issue 1
Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation
journal, February 2021
- Tillu, Vikas A.; Rae, James; Gao, Ya
- Nature Communications, Vol. 12, Issue 1
A genomic and epigenomic atlas of prostate cancer in Asian populations
journal, March 2020
- Li, Jing; Xu, Chuanliang; Lee, Hyung Joo
- Nature, Vol. 580, Issue 7801
Overview of the CCP4 suite and current developments.
text, January 2011
- Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D.
- Apollo - University of Cambridge Repository
Structure of human O-GlcNAc transferase and its complex with a peptide substrate
text, January 2011
- Lazarus, M. B.; Nam, Y.; Jiang, J.
- Protein Data Bank, Rutgers University
Regulation of mammalian Notch signaling and embryonic development by the protein O-glucosyltransferase Rumi
journal, August 2011
- Fernandez-Valdivia, Rodrigo; Takeuchi, Hideyuki; Samarghandi, Amin
- Developmental Biology, Vol. 356, Issue 1
PHENIX: a comprehensive Python-based system for macromolecular structure solution.
text, January 2010
- Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
- Apollo - University of Cambridge Repository
A faithful JAGGED1 haploinsufficiency mouse model of arteriohepatic dysplasia (Alagille syndrome) after all
journal, December 2015
- Huppert, Stacey S.
- Hepatology, Vol. 63, Issue 2
Novel roles for O-linked glycans in protein folding
journal, September 2014
- Vasudevan, Deepika; Haltiwanger, Robert S.
- Glycoconjugate Journal, Vol. 31, Issue 6-7
Down-regulated expression of Notch signaling molecules in human endometrial cancer
journal, January 2013
- Jonusiene, Violeta; Sasnauskiene, Ausra; Lachej, Nadezda
- Medical Oncology, Vol. 30, Issue 1
Mutations in POGLUT1, Encoding Protein O-Glucosyltransferase 1, Cause Autosomal-Dominant Dowling-Degos Disease
journal, January 2014
- Basmanav, F. Buket; Oprisoreanu, Ana-Maria; Pasternack, Sandra M.
- The American Journal of Human Genetics, Vol. 94, Issue 1
From Fly Wings to Targeted Cancer Therapies: A Centennial for Notch Signaling
journal, March 2014
- Ntziachristos, Panagiotis; Lim, Jing Shan; Sage, Julien
- Cancer Cell, Vol. 25, Issue 3
The Canonical Notch Signaling Pathway: Unfolding the Activation Mechanism
journal, April 2009
- Kopan, Raphael; Ilagan, Ma. Xenia G.
- Cell, Vol. 137, Issue 2
Structural Evidence of a Passive Base-flipping Mechanism for AGT, an Unusual GT-B Glycosyltransferase
journal, September 2005
- Larivière, Laurent; Sommer, Nicole; Moréra, Solange
- Journal of Molecular Biology, Vol. 352, Issue 1
Fringe benefits: Functional and structural impacts of O-glycosylation on the extracellular domain of Notch receptors
journal, October 2011
- Rana, Nadia A.; Haltiwanger, Robert S.
- Current Opinion in Structural Biology, Vol. 21, Issue 5
Taking HSCs Down a Notch in Leukemia
journal, June 2011
- Walkley, Carl R.; Izon, David J.; Purton, Louise E.
- Cell Stem Cell, Vol. 8, Issue 6
Crystal Structures of the T4 Phage β-Glucosyltransferase and the D100A Mutant in Complex with UDP-glucose: Glucose Binding and Identification of the Catalytic Base for a Direct Displacement Mechanism
journal, July 2003
- Larivière, Laurent; Gueguen-Chaignon, Virginie; Moréra, Solange
- Journal of Molecular Biology, Vol. 330, Issue 5
Structure of human O-GlcNAc transferase and its complex with a peptide substrate
journal, January 2011
- Lazarus, Michael B.; Nam, Yunsun; Jiang, Jiaoyang
- Nature, Vol. 469, Issue 7331
O-GlcNAc transferase invokes nucleotide sugar pyrophosphate participation in catalysis
journal, October 2012
- Schimpl, Marianne; Zheng, Xiaowei; Borodkin, Vladimir S.
- Nature Chemical Biology, Vol. 8, Issue 12
Structural snapshots of the reaction coordinate for O-GlcNAc transferase
journal, October 2012
- Lazarus, Michael B.; Jiang, Jiaoyang; Gloster, Tracey M.
- Nature Chemical Biology, Vol. 8, Issue 12
A proactive role of water molecules in acceptor recognition by protein O-fucosyltransferase 2
journal, February 2016
- Valero-González, Jessika; Leonhard-Melief, Christina; Lira-Navarrete, Erandi
- Nature Chemical Biology, Vol. 12, Issue 4
O -Glucose Trisaccharide Is Present at High but Variable Stoichiometry at Multiple Sites on Mouse Notch1
journal, July 2011
- Rana, Nadia A.; Nita-Lazar, Aleksandra; Takeuchi, Hideyuki
- Journal of Biological Chemistry, Vol. 286, Issue 36
The carbohydrate-active enzymes database (CAZy) in 2013
journal, November 2013
- Lombard, Vincent; Golaconda Ramulu, Hemalatha; Drula, Elodie
- Nucleic Acids Research, Vol. 42, Issue D1
Evolution of distinct EGF domains with specific functions
journal, April 2005
- Wouters, Merridee A.; Rigoutsos, Isidore; Chu, Carmen K.
- Protein Science, Vol. 14, Issue 4
Exome Sequencing of Head and Neck Squamous Cell Carcinoma Reveals Inactivating Mutations in NOTCH1
journal, July 2011
- Agrawal, N.; Frederick, M. J.; Pickering, C. R.
- Science, Vol. 333, Issue 6046
HCF-1 Is Cleaved in the Active Site of O-GlcNAc Transferase
journal, December 2013
- Lazarus, M. B.; Jiang, J.; Kapuria, V.
- Science, Vol. 342, Issue 6163
Glycosyltransferases: Structures, Functions, and Mechanisms
journal, June 2008
- Lairson, L. L.; Henrissat, B.; Davies, G. J.
- Annual Review of Biochemistry, Vol. 77, Issue 1
Generalized Solar Lentigines in a Patient with a History of Radon Exposure
journal, January 2010
- Mauerer, Andreas; Betz, Regina C.; Pasternack, Sandra M.
- Dermatology, Vol. 221, Issue 3
Negative Regulation of Notch Signaling by Xylose
journal, June 2013
- Lee, Tom V.; Sethi, Maya K.; Leonardi, Jessica
- PLoS Genetics, Vol. 9, Issue 6
The Protein O-glucosyltransferase Rumi Modifies Eyes Shut to Promote Rhabdomere Separation in Drosophila
journal, November 2014
- Haltom, Amanda R.; Lee, Tom V.; Harvey, Beth M.
- PLoS Genetics, Vol. 10, Issue 11
Protein O-Glucosyltransferase 1 (POGLUT1) Promotes Mouse Gastrulation through Modification of the Apical Polarity Protein CRUMBS2
journal, October 2015
- Ramkumar, Nitya; Harvey, Beth M.; Lee, Jeffrey D.
- PLOS Genetics, Vol. 11, Issue 10
Structural Insights into the Mechanism of Protein O-Fucosylation
journal, September 2011
- Lira-Navarrete, Erandi; Valero-González, Jessika; Villanueva, Raquel
- PLoS ONE, Vol. 6, Issue 9
Works referencing / citing this record:
POGLUT1 biallelic mutations cause myopathy with reduced satellite cells, α-dystroglycan hypoglycosylation and a distinctive radiological pattern
journal, January 2020
- Servián-Morilla, E.; Cabrera-Serrano, M.; Johnson, K.
- Acta Neuropathologica, Vol. 139, Issue 3
Structural basis of Notch O-glucosylation and O–xylosylation by mammalian protein–O-glucosyltransferase 1 (POGLUT1)
journal, August 2017
- Li, Zhijie; Fischer, Michael; Satkunarajah, Malathy
- Nature Communications, Vol. 8, Issue 1
Structural basis of protein arginine rhamnosylation by glycosyltransferase EarP
journal, February 2018
- Sengoku, Toru; Suzuki, Takehiro; Dohmae, Naoshi
- Nature Chemical Biology, Vol. 14, Issue 4
Legionella effector SetA as a general O-glucosyltransferase for eukaryotic proteins
journal, January 2019
- Gao, Ling; Song, Qitao; Liang, Hao
- Nature Chemical Biology, Vol. 15, Issue 3
Emerging structural insights into glycosyltransferase-mediated synthesis of glycans
journal, August 2019
- Moremen, Kelley W.; Haltiwanger, Robert S.
- Nature Chemical Biology, Vol. 15, Issue 9
O -Glycosylation modulates the stability of epidermal growth factor-like repeats and thereby regulates Notch trafficking
journal, July 2017
- Takeuchi, Hideyuki; Yu, Hongjun; Hao, Huilin
- Journal of Biological Chemistry, Vol. 292, Issue 38
Effects of Notch glycosylation on health and diseases
journal, December 2019
- Urata, Yusuke; Takeuchi, Hideyuki
- Development, Growth & Differentiation, Vol. 62, Issue 1
Notch Signaling in Development, Tissue Homeostasis, and Disease
journal, October 2017
- Siebel, Chris; Lendahl, Urban
- Physiological Reviews, Vol. 97, Issue 4
Structural basis of Notch O-glucosylation and O–xylosylation by mammalian protein–O-glucosyltransferase 1 (POGLUT1)
journal, August 2017
- Li, Zhijie; Fischer, Michael; Satkunarajah, Malathy
- Nature Communications, Vol. 8, Issue 1