A Human-Infecting H10N8 Influenza Virus Retains a Strong Preference for Avian-type Receptors

Zhang, Heng; de Vries, Robert P.; Tzarum, Netanel; Zhu, Xueyong; Yu, Wenli; McBride, Ryan; Paulson, James C.; Wilson, Ian A.

doi:10.1016/j.chom.2015.02.006

Title: A Human-Infecting H10N8 Influenza Virus Retains a Strong Preference for Avian-type Receptors

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Abstract

Recent avian-origin H10N8 influenza A viruses that have infected humans pose a potential pandemic threat. Alterations in the viral surface glycoprotein, hemagglutinin (HA), typically are required for influenza A viruses to cross the species barrier for adaptation to a new host, but whether H10N8 contains adaptations supporting human infection remains incompletely understood. In this paper, we investigated whether H10N8 HA can bind human receptors. Sialoside glycan microarray analysis showed that the H10 HA retains a strong preference for avian receptor analogs and negligible binding to human receptor analogs. Crystal structures of H10 HA with avian and human receptor analogs revealed the basis for preferential recognition of avian-like receptors. Furthermore, introduction of mutations into the H10 receptor-binding site (RBS) known to convert other HA subtypes from avian to human receptor specificity failed to switch preference to human receptors. In conclusion, collectively these findings suggest that the current H10N8 human isolates are poorly adapted for efficient human-to-human transmission.

Authors:: Zhang, Heng; de Vries, Robert P.; Tzarum, Netanel; Zhu, Xueyong; Yu, Wenli; McBride, Ryan; Paulson, James C.; Wilson, Ian A.

Publication Date:: Sun Mar 01 00:00:00 EST 2015

Research Org.:: The Scripps Research Inst., La Jolla, CA (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Inst. of Health (NIH) (United States)

OSTI Identifier:: 1241402

Alternate Identifier(s):: OSTI ID: 1233921; OSTI ID: 1347138

Grant/Contract Number:: R56 AI099275; AI099274; Y1-CO-1020; Y1-GM-1104; GM62116; P41GM103393; P41RR001209

Resource Type:: Published Article

Journal Name:: Cell Host & Microbe

Additional Journal Information:: Journal Name: Cell Host & Microbe Journal Volume: 17 Journal Issue: 3; Journal ID: ISSN 1931-3128

Publisher:: Elsevier

Country of Publication:: Netherlands

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats


                    Zhang, Heng, de Vries, Robert P., Tzarum, Netanel, Zhu, Xueyong, Yu, Wenli, McBride, Ryan, Paulson, James C., and Wilson, Ian A. A Human-Infecting H10N8 Influenza Virus Retains a Strong Preference for Avian-type Receptors.  Netherlands: N. p., 2015. 
Web.  doi:10.1016/j.chom.2015.02.006.

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                    Zhang, Heng, de Vries, Robert P., Tzarum, Netanel, Zhu, Xueyong, Yu, Wenli, McBride, Ryan, Paulson, James C., & Wilson, Ian A. A Human-Infecting H10N8 Influenza Virus Retains a Strong Preference for Avian-type Receptors.  Netherlands.  https://doi.org/10.1016/j.chom.2015.02.006

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                    Zhang, Heng, de Vries, Robert P., Tzarum, Netanel, Zhu, Xueyong, Yu, Wenli, McBride, Ryan, Paulson, James C., and Wilson, Ian A. Sun .  
"A Human-Infecting H10N8 Influenza Virus Retains a Strong Preference for Avian-type Receptors".  Netherlands.  https://doi.org/10.1016/j.chom.2015.02.006.

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@article{osti_1241402,

  title        = {A Human-Infecting H10N8 Influenza Virus Retains a Strong Preference for Avian-type Receptors},

  author       = {Zhang, Heng and de Vries, Robert P. and Tzarum, Netanel and Zhu, Xueyong and Yu, Wenli and McBride, Ryan and Paulson, James C. and Wilson, Ian A.},

  abstractNote = {Recent avian-origin H10N8 influenza A viruses that have infected humans pose a potential pandemic threat. Alterations in the viral surface glycoprotein, hemagglutinin (HA), typically are required for influenza A viruses to cross the species barrier for adaptation to a new host, but whether H10N8 contains adaptations supporting human infection remains incompletely understood. In this paper, we investigated whether H10N8 HA can bind human receptors. Sialoside glycan microarray analysis showed that the H10 HA retains a strong preference for avian receptor analogs and negligible binding to human receptor analogs. Crystal structures of H10 HA with avian and human receptor analogs revealed the basis for preferential recognition of avian-like receptors. Furthermore, introduction of mutations into the H10 receptor-binding site (RBS) known to convert other HA subtypes from avian to human receptor specificity failed to switch preference to human receptors. In conclusion, collectively these findings suggest that the current H10N8 human isolates are poorly adapted for efficient human-to-human transmission.},

  doi          = {10.1016/j.chom.2015.02.006},

  journal      = {Cell Host & Microbe},

  number       = 3,

  volume       = 17,

  place        = {Netherlands},

  year         = {Sun Mar 01 00:00:00 EST 2015},

  month        = {Sun Mar 01 00:00:00 EST 2015}

}

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