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Title: Cas9 gRNA engineering for genome editing, activation and repression

Here we demonstrate that by altering the length of Cas9-associated guide RNA(gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.
Authors:
 [1] ;  [2] ;  [3] ;  [1] ;  [4] ;  [5] ;  [5] ;  [3] ; ORCiD logo [6] ; ORCiD logo [7] ;  [3] ;  [3] ;  [1] ;  [8] ;  [1] ;  [5]
  1. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
  2. Harvard Univ., Cambridge, MA (United States); Massachusetts Hospital, Boston, MA (United States); Harvard Medical School, Boston, MA (United States)
  3. Harvard Univ., Cambridge, MA (United States)
  4. Harvard Medical School, Boston, MA (United States)
  5. Harvard Univ., Cambridge, MA (United States); Harvard Medical School, Boston, MA (United States)
  6. Raytheon BBN Technologies, Cambridge, MA (United States)
  7. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Harvard Univ., Cambridge, MA (United States); Harvard Medical School, Boston, MA (United States)
  8. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Harvard Univ., Cambridge, MA (United States); Broad Institute of MIT and Harvard, Cambridge, MA (United States); Harvard-MIT Program in Health Sciences and Technology, Cambridge, MA (United States)
Publication Date:
Grant/Contract Number:
FG02-02ER63445
Type:
Accepted Manuscript
Journal Name:
Nature Methods
Additional Journal Information:
Journal Volume: 12; Journal Issue: 11; Journal ID: ISSN 1548-7091
Publisher:
Nature Publishing Group
Research Org:
Harvard Univ., Cambridge, MA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1347131