Engineering the production of conjugated fatty acids in Arabidopsis thaliana leaves
Abstract
Summary The seeds of many nondomesticated plant species synthesize oils containing high amounts of a single unusual fatty acid, many of which have potential usage in industry. Despite the identification of enzymes for unusual oxidized fatty acid synthesis, the production of these fatty acids in engineered seeds remains low and is often hampered by their inefficient exclusion from phospholipids. Recent studies have established the feasibility of increasing triacylglycerol content in plant leaves, which provides a novel approach for increasing energy density of biomass crops. Here, we determined whether the fatty acid composition of leaf oil could be engineered to accumulate unusual fatty acids. Eleostearic acid ( ESA ) is a conjugated fatty acid produced in seeds of the tung tree ( Vernicia fordii ) and has both industrial and nutritional end‐uses. Arabidopsis thaliana lines with elevated leaf oil were first generated by transforming wild‐type, cgi‐58 or pxa1 mutants (the latter two of which contain mutations disrupting fatty acid breakdown) with the diacylglycerol acyltransferases ( DGAT 1 or DGAT 2 ) and/or oleosin genes from tung. High‐leaf‐oil plant lines were then transformed with tung FADX , which encodes the fatty acid desaturase/conjugase responsible for ESA synthesis. Analysis of lipids in leavesmore »
- Authors:
-
- USDA‐ARS US Arid‐Land Agricultural Research Center Maricopa AZ USA
- USDA‐ARS Southern Regional Research Center New Orleans LA USA
- Department of Molecular and Cellular Biology University of Guelph Guelph ON Canada
- Department of Biological Sciences University of North Texas Denton TX USA
- Publication Date:
- Research Org.:
- Univ. of North Texas, Denton, TX (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1347058
- Alternate Identifier(s):
- OSTI ID: 1347059; OSTI ID: 1465812
- Grant/Contract Number:
- DE‐FG02‐09ER64812; DE‐SC0016536; SC0016536
- Resource Type:
- Published Article
- Journal Name:
- Plant Biotechnology Journal
- Additional Journal Information:
- Journal Name: Plant Biotechnology Journal Journal Volume: 15 Journal Issue: 8; Journal ID: ISSN 1467-7644
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Arabidopsis thaliana; conjugated fatty acids; bio‐based feedstocks; biofuels; oil in leaves; Vernicia fordii
Citation Formats
Yurchenko, Olga, Shockey, Jay M., Gidda, Satinder K., Silver, Maxwell I., Chapman, Kent D., Mullen, Robert T., and Dyer, John M. Engineering the production of conjugated fatty acids in Arabidopsis thaliana leaves. United Kingdom: N. p., 2017.
Web. doi:10.1111/pbi.12695.
Yurchenko, Olga, Shockey, Jay M., Gidda, Satinder K., Silver, Maxwell I., Chapman, Kent D., Mullen, Robert T., & Dyer, John M. Engineering the production of conjugated fatty acids in Arabidopsis thaliana leaves. United Kingdom. https://doi.org/10.1111/pbi.12695
Yurchenko, Olga, Shockey, Jay M., Gidda, Satinder K., Silver, Maxwell I., Chapman, Kent D., Mullen, Robert T., and Dyer, John M. Wed .
"Engineering the production of conjugated fatty acids in Arabidopsis thaliana leaves". United Kingdom. https://doi.org/10.1111/pbi.12695.
@article{osti_1347058,
title = {Engineering the production of conjugated fatty acids in Arabidopsis thaliana leaves},
author = {Yurchenko, Olga and Shockey, Jay M. and Gidda, Satinder K. and Silver, Maxwell I. and Chapman, Kent D. and Mullen, Robert T. and Dyer, John M.},
abstractNote = {Summary The seeds of many nondomesticated plant species synthesize oils containing high amounts of a single unusual fatty acid, many of which have potential usage in industry. Despite the identification of enzymes for unusual oxidized fatty acid synthesis, the production of these fatty acids in engineered seeds remains low and is often hampered by their inefficient exclusion from phospholipids. Recent studies have established the feasibility of increasing triacylglycerol content in plant leaves, which provides a novel approach for increasing energy density of biomass crops. Here, we determined whether the fatty acid composition of leaf oil could be engineered to accumulate unusual fatty acids. Eleostearic acid ( ESA ) is a conjugated fatty acid produced in seeds of the tung tree ( Vernicia fordii ) and has both industrial and nutritional end‐uses. Arabidopsis thaliana lines with elevated leaf oil were first generated by transforming wild‐type, cgi‐58 or pxa1 mutants (the latter two of which contain mutations disrupting fatty acid breakdown) with the diacylglycerol acyltransferases ( DGAT 1 or DGAT 2 ) and/or oleosin genes from tung. High‐leaf‐oil plant lines were then transformed with tung FADX , which encodes the fatty acid desaturase/conjugase responsible for ESA synthesis. Analysis of lipids in leaves revealed that ESA was efficiently excluded from phospholipids, and co‐expression of tung FADX and DGAT 2 promoted a synergistic increase in leaf oil content and ESA accumulation. Taken together, these results provide a new approach for increasing leaf oil content that is coupled with accumulation of unusual fatty acids. Implications for production of biofuels, bioproducts, and plant–pest interactions are discussed.},
doi = {10.1111/pbi.12695},
journal = {Plant Biotechnology Journal},
number = 8,
volume = 15,
place = {United Kingdom},
year = {Wed Mar 15 00:00:00 EDT 2017},
month = {Wed Mar 15 00:00:00 EDT 2017}
}
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Works referenced in this record:
Senescence-inducible LEC2 enhances triacylglycerol accumulation in leaves without negatively affecting plant growth
journal, March 2015
- Kim, Hyun Uk; Lee, Kyeong-Ryeol; Jung, Su-Jin
- Plant Biotechnology Journal, Vol. 13, Issue 9
Oxidative Stability of Conjugated Linolenic Acids
journal, May 2009
- Yang, Lin; Cao, Ying; Chen, Jing-Nan
- Journal of Agricultural and Food Chemistry, Vol. 57, Issue 10
Fluxes through the prokaryotic and eukaryotic pathways of lipid synthesis in the ‘16:3’ plant Arabidopsis thaliana
journal, April 1986
- Browse, J.; Warwick, N.; Somerville, C. R.
- Biochemical Journal, Vol. 235, Issue 1
Conjugated linolenic acids and their bioactivities: a review
journal, January 2014
- Yuan, Gao-Feng; Chen, Xiao-E.; Li, Duo
- Food & Function, Vol. 5, Issue 7
Arabidopsis SEIPIN Proteins Modulate Triacylglycerol Accumulation and Influence Lipid Droplet Proliferation
journal, September 2015
- Cai, Yingqi; Goodman, Joel M.; Pyc, Michal
- The Plant Cell, Vol. 27, Issue 9
Plant triacylglycerols as feedstocks for the production of biofuels
journal, May 2008
- Durrett, Timothy P.; Benning, Christoph; Ohlrogge, John
- The Plant Journal, Vol. 54, Issue 4, p. 593-607
The α/β Hydrolase CGI-58 and Peroxisomal Transport Protein PXA1 Coregulate Lipid Homeostasis and Signaling in Arabidopsis
journal, May 2013
- Park, Sunjung; Gidda, Satinder K.; James, Christopher N.
- The Plant Cell, Vol. 25, Issue 5, p. 1726-1739
Increasing the energy density of vegetative tissues by diverting carbon from starch to oil biosynthesis in transgenic Arabidopsis
journal, February 2011
- Sanjaya, ; Durrett, Timothy P.; Weise, Sean E.
- Plant Biotechnology Journal, Vol. 9, Issue 8, p. 874-883
Molecular properties of the class III subfamily of acyl-coenyzme A binding proteins from tung tree (Vernicia fordii)
journal, April 2013
- Pastor, Steven; Sethumadhavan, Kandan; Ullah, Abul H. J.
- Plant Science, Vol. 203-204
Arabidopsis Lipins, PDAT1 Acyltransferase, and SDP1 Triacylglycerol Lipase Synergistically Direct Fatty Acids toward β-Oxidation, Thereby Maintaining Membrane Lipid Homeostasis
journal, October 2014
- Fan, Jilian; Yan, Chengshi; Roston, Rebecca
- The Plant Cell, Vol. 26, Issue 10
The Phosphatidylcholine Diacylglycerol Cholinephosphotransferase Is Required for Efficient Hydroxy Fatty Acid Accumulation in Transgenic Arabidopsis
journal, February 2012
- Hu, Zhaohui; Ren, Zhonghai; Lu, Chaofu
- Plant Physiology, Vol. 158, Issue 4
Conjugated fatty acids accumulate to high levels in phospholipids of metabolically engineered soybean and Arabidopsis seeds
journal, June 2006
- Cahoon, Edgar B.; Dietrich, Charles R.; Meyer, Knut
- Phytochemistry, Vol. 67, Issue 12
Understanding and manipulating plant lipid composition: Metabolic engineering leads the way
journal, June 2014
- Napier, Johnathan A.; Haslam, Richard P.; Beaudoin, Frederic
- Current Opinion in Plant Biology, Vol. 19
The Arabidopsis pxa1 Mutant Is Defective in an ATP-Binding Cassette Transporter-Like Protein Required for Peroxisomal Fatty Acid β-Oxidation
journal, November 2001
- Zolman, Bethany K.; Silva, Illeana D.; Bartel, Bonnie
- Plant Physiology, Vol. 127, Issue 3
The pathway of triacylglycerol synthesis through phosphatidylcholine in Arabidopsis produces a bottleneck for the accumulation of unusual fatty acids in transgenic seeds: PC is a bottleneck in HFA-TAG synthesis
journal, August 2011
- Bates, Philip D.; Browse, John
- The Plant Journal, Vol. 68, Issue 3
12-Oxo-Phytodienoic Acid Accumulation during Seed Development Represses Seed Germination in Arabidopsis
journal, February 2011
- Dave, Anuja; Hernández, M. Luisa; He, Zhesi
- The Plant Cell, Vol. 23, Issue 2
Ped3p is a Peroxisomal ATP-Binding Cassette Transporter that might Supply Substrates for Fatty Acid β-Oxidation
journal, January 2002
- Hayashi, Makoto; Nito, Kazumasa; Takei-Hoshi, Rie
- Plant and Cell Physiology, Vol. 43, Issue 1
Engineering Oil Accumulation in Vegetative Tissue
book, January 2016
- Weselake, Randall J.
- Industrial Oil Crops
Non-vesicular and vesicular lipid trafficking involving plastids
journal, June 2006
- Benning, Christoph; Xu, Changcheng; Awai, Koichiro
- Current Opinion in Plant Biology, Vol. 9, Issue 3
Biosynthetic origin of conjugated double bonds: Production of fatty acid components of high-value drying oils in transgenic soybean embryos
journal, October 1999
- Cahoon, E. B.; Carlson, T. J.; Ripp, K. G.
- Proceedings of the National Academy of Sciences, Vol. 96, Issue 22
FAX1, a Novel Membrane Protein Mediating Plastid Fatty Acid Export
journal, February 2015
- Li, Nannan; Gügel, Irene Luise; Giavalisco, Patrick
- PLOS Biology, Vol. 13, Issue 2
Expression of Lauroyl–Acyl Carrier Protein Thioesterase in Brassica napus Seeds Induces Pathways for Both Fatty Acid Oxidation and Biosynthesis and Implies a Set Point for Triacylglycerol Accumulation
journal, April 1998
- Eccleston, Victoria S.; Ohlrogge, John B.
- The Plant Cell, Vol. 10, Issue 4
Jasmonic Acid Levels Are Reduced in COMATOSE ATP-Binding Cassette Transporter Mutants. Implications for Transport of Jasmonate Precursors into Peroxisomes
journal, March 2005
- Theodoulou, Frederica L.; Job, Kathleen; Slocombe, Steven P.
- Plant Physiology, Vol. 137, Issue 3
Step changes in leaf oil accumulation via iterative metabolic engineering
journal, January 2017
- Vanhercke, Thomas; Divi, Uday K.; El Tahchy, Anna
- Metabolic Engineering, Vol. 39
Lipid turnover during senescence
journal, May 2013
- Troncoso-Ponce, Manuel A.; Cao, Xia; Yang, Zhenle
- Plant Science, Vol. 205-206, p. 13-19
Metabolic engineering of hydroxy fatty acid production in plants: RcDGAT2 drives dramatic increases in ricinoleate levels in seed oil
journal, October 2008
- Burgal, Julie; Shockey, Jay; Lu, Chaofu
- Plant Biotechnology Journal, Vol. 6, Issue 8
In Vivo Packaging of Triacylglycerols Enhances Arabidopsis Leaf Biomass and Energy Density
journal, April 2013
- Winichayakul, Somrutai; Scott, Richard William; Roldan, Marissa
- Plant Physiology, Vol. 162, Issue 2
Phospholipid:diacylglycerol acyltransferase-mediated triacylglycerol biosynthesis is crucial for protection against fatty acid-induced cell death in growing tissues of Arabidopsis
journal, November 2013
- Fan, Jilian; Yan, Chengshi; Xu, Changcheng
- The Plant Journal, Vol. 76, Issue 6, p. 930-942
Occurrence of unusual fatty acids in plants
journal, January 1971
- Smith, C. R.
- Progress in the Chemistry of Fats and other Lipids, Vol. 11
Commentary: Why don’t plant leaves get fat?
journal, June 2013
- Chapman, Kent D.; Dyer, John M.; Mullen, Robert T.
- Plant Science, Vol. 207
A Gateway Cloning Vector Set for High-Throughput Functional Analysis of Genes in Planta
journal, September 2003
- Curtis, Mark D.; Grossniklaus, Ueli
- Plant Physiology, Vol. 133, Issue 2, p. 462-469
Castor oil induces laxation and uterus contraction via ricinoleic acid activating prostaglandin EP3 receptors
journal, May 2012
- Tunaru, S.; Althoff, T. F.; Nusing, R. M.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 23
Vernonia DGATs increase accumulation of epoxy fatty acids in oil
journal, February 2010
- Li, Runzhi; Yu, Keshun; Hatanaka, Tomoko
- Plant Biotechnology Journal, Vol. 8, Issue 2
Expression of Castor LPAT2 Enhances Ricinoleic Acid Content at the sn-2 Position of Triacylglycerols in Lesquerella Seed
journal, April 2016
- Chen, Grace; van Erp, Harrie; Martin-Moreno, Jose
- International Journal of Molecular Sciences, Vol. 17, Issue 4
Dissection of Arabidopsis Bax Inhibitor-1 Suppressing Bax–, Hydrogen Peroxide–, and Salicylic Acid–Induced Cell Death
journal, December 2003
- Kawai-Yamada, Maki; Ohori, Yuri; Uchimiya, Hirofumi
- The Plant Cell, Vol. 16, Issue 1
Arabidopsis GPAT9 contributes to synthesis of intracellular glycerolipids but not surface lipids
journal, June 2016
- Singer, Stacy D.; Chen, Guanqun; Mietkiewska, Elzbieta
- Journal of Experimental Botany, Vol. 67, Issue 15
Oils and Fats as Renewable Raw Materials in Chemistry
journal, March 2011
- Biermann, Ursula; Bornscheuer, Uwe; Meier, Michael A. R.
- Angewandte Chemie International Edition, Vol. 50, Issue 17
Identification of Non-Heme Diiron Proteins That Catalyze Triple Bond and Epoxy Group Formation
journal, May 1998
- Lee, M.
- Science, Vol. 280, Issue 5365
Metabolic engineering of sugarcane to accumulate energy-dense triacylglycerols in vegetative biomass
journal, June 2015
- Zale, Janice; Jung, Je Hyeong; Kim, Jae Yoon
- Plant Biotechnology Journal, Vol. 14, Issue 2
Identification of Arabidopsis GPAT9 (At5g60620) as an Essential Gene Involved in Triacylglycerol Biosynthesis
journal, November 2015
- Shockey, Jay; Regmi, Anushobha; Cotton, Kimberly
- Plant Physiology, Vol. 170, Issue 1
Fatty acid biosynthesis redirected to medium chains in transgenic oilseed plants
journal, July 1992
- Voelker, T.; Worrell, A.; Anderson, L.
- Science, Vol. 257, Issue 5066
Expression in yeast and tobacco of plant cDNAs encoding acyl CoA:diacylglycerol acyltransferase: Plant cDNAs encoding diacylglycerol acyltransferase
journal, January 2000
- Bouvier-Navé, Pierrette; Benveniste, Pierre; Oelkers, Peter
- European Journal of Biochemistry, Vol. 267, Issue 1
Metabolic engineering of biomass for high energy density: oilseed-like triacylglycerol yields from plant leaves
journal, October 2013
- Vanhercke, Thomas; El Tahchy, Anna; Liu, Qing
- Plant Biotechnology Journal, Vol. 12, Issue 2
Disruption of the Arabidopsis CGI-58 homologue produces Chanarin–Dorfman-like lipid droplet accumulation in plants
journal, September 2010
- James, Christopher N.; Horn, Patrick J.; Case, Charlene R.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 41, p. 17833-17838
Fatty acid synthesis is inhibited by inefficient utilization of unusual fatty acids for glycerolipid assembly
journal, January 2014
- Bates, P. D.; Johnson, S. R.; Cao, X.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 3
Engineering oilseeds for sustainable production of industrial and nutritional feedstocks: solving bottlenecks in fatty acid flux
journal, June 2007
- Cahoon, Edgar B.; Shockey, Jay M.; Dietrich, Charles R.
- Current Opinion in Plant Biology, Vol. 10, Issue 3
An oleate 12-hydroxylase from Ricinus communis L. is a fatty acyl desaturase homolog.
journal, July 1995
- van de Loo, F. J.; Broun, P.; Turner, S.
- Proceedings of the National Academy of Sciences, Vol. 92, Issue 15
Control of germination and lipid mobilization by COMATOSE, the Arabidopsis homologue of human ALDP
journal, June 2002
- Footitt, S.
- The EMBO Journal, Vol. 21, Issue 12
In Vivo Studies of the Biosynthesis of [alpha]-Eleostearic Acid in the Seed of Momordica charantia L
journal, April 1997
- Liu, L.; Hammond, E. G.; Nikolau, B. J.
- Plant Physiology, Vol. 113, Issue 4
A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures
journal, July 1962
- Murashige, Toshio; Skoog, Folke
- Physiologia Plantarum, Vol. 15, Issue 3, p. 473-497
Reducing Isozyme Competition Increases Target Fatty Acid Accumulation in Seed Triacylglycerols of Transgenic Arabidopsis
journal, March 2015
- van Erp, Harrie; Shockey, Jay; Zhang, Meng
- Plant Physiology, Vol. 168, Issue 1
Plant Acyl-CoA:Lysophosphatidylcholine Acyltransferases (LPCATs) Have Different Specificities in Their Forward and Reverse Reactions
journal, November 2013
- Lager, Ida; Yilmaz, Jenny Lindberg; Zhou, Xue-Rong
- Journal of Biological Chemistry, Vol. 288, Issue 52
FAD2 and FAD3 Desaturases Form Heterodimers That Facilitate Metabolic Channeling in Vivo
journal, May 2014
- Lou, Ying; Schwender, Jorg; Shanklin, John
- Journal of Biological Chemistry, Vol. 289, Issue 26
Structure and occurrence of unusual fatty acids in minor seed oils
journal, January 1980
- Badami, R.; Patil, K.
- Progress in Lipid Research, Vol. 19, Issue 3-4
Identification, Classification and Differential Expression of Oleosin Genes in Tung Tree (Vernicia fordii)
journal, February 2014
- Cao, Heping; Zhang, Lin; Tan, Xiaofeng
- PLoS ONE, Vol. 9, Issue 2
Transgenic expression of a Δ12-epoxygenase gene in Arabidopsis seeds inhibits accumulation of linoleic acid
journal, April 2001
- Singh, Surinder; Thomaeus, Stefan; Lee, Michael
- Planta, Vol. 212, Issue 5-6
The Impact of Biofuels on Commodity Food Prices: Assessment of Findings
journal, June 2012
- Zilberman, D.; Hochman, G.; Rajagopal, D.
- American Journal of Agricultural Economics, Vol. 95, Issue 2
Metabolic engineering of plant oils and waxes for use as industrial feedstocks
journal, November 2012
- Vanhercke, Thomas; Wood, Craig C.; Stymne, Sten
- Plant Biotechnology Journal, Vol. 11, Issue 2
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
journal, December 1998
- Clough, Steven J.; Bent, Andrew F.
- The Plant Journal, Vol. 16, Issue 6, p. 735-743
The ABC Transporter PXA1 and Peroxisomal -Oxidation Are Vital for Metabolism in Mature Leaves of Arabidopsis during Extended Darkness
journal, September 2009
- Kunz, H.-H.; Scharnewski, M.; Feussner, K.
- The Plant Cell Online, Vol. 21, Issue 9, p. 2733-2749
Acyl Editing and Headgroup Exchange Are the Major Mechanisms That Direct Polyunsaturated Fatty Acid Flux into Triacylglycerols
journal, August 2012
- Bates, Philip D.; Fatihi, Abdelhak; Snapp, Anna R.
- Plant Physiology, Vol. 160, Issue 3
Lipid extraction of tissues with a low-toxicity solvent
journal, October 1978
- Hara, Atsushi; Radin, Norman S.
- Analytical Biochemistry, Vol. 90, Issue 1
Development and analysis of a highly flexible multi-gene expression system for metabolic engineering in Arabidopsis seeds and other plant tissues
journal, August 2015
- Shockey, Jay; Mason, Catherine; Gilbert, Matthew
- Plant Molecular Biology, Vol. 89, Issue 1-2
High-value oils from plants
journal, May 2008
- Dyer, John M.; Stymne, Sten; Green, Allan G.
- The Plant Journal, Vol. 54, Issue 4
Conjugated linoleic acid changes swine performance and carcass composition.
journal, January 2001
- Thiel-Cooper, R. L.; Parrish, F. C.; Sparks, J. C.
- Journal of Animal Science, Vol. 79, Issue 7
Triacylglycerol Metabolism, Function, and Accumulation in Plant Vegetative Tissues
journal, April 2016
- Xu, Changcheng; Shanklin, John
- Annual Review of Plant Biology, Vol. 67, Issue 1
Heterologous expression of Arabidopsis C-repeat binding factor 3 (AtCBF3) and cold-regulated 15A (AtCOR15A) enhanced chilling tolerance in transgenic eggplant (Solanum melongena L.)
journal, August 2014
- Wan, Faxiang; Pan, Yu; Li, Jinghua
- Plant Cell Reports, Vol. 33, Issue 12
Arabidopsis FAD2 gene encodes the enzyme that is essential for polyunsaturated lipid synthesis.
journal, January 1994
- Okuley, J.; Lightner, J.; Feldmann, K.
- The Plant Cell, Vol. 6, Issue 1
DGAT1 and PDAT1 Acyltransferases Have Overlapping Functions in Arabidopsis Triacylglycerol Biosynthesis and Are Essential for Normal Pollen and Seed Development
journal, December 2009
- Zhang, Meng; Fan, Jilian; Taylor, David C.
- The Plant Cell, Vol. 21, Issue 12
Fungal responsive fatty acid acetylenases occur widely in evolutionarily distant plant families
journal, June 2003
- Cahoon, Edgar B.; Schnurr, Judy A.; Huffman, Errol A.
- The Plant Journal, Vol. 34, Issue 5
Understanding the control of acyl flux through the lipid metabolic network of plant oil biosynthesis
journal, September 2016
- Bates, Philip D.
- Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol. 1861, Issue 9
Biogenesis and functions of lipid droplets in plants: Thematic Review Series: Lipid Droplet Synthesis and Metabolism: from Yeast to Man
journal, November 2011
- Chapman, Kent D.; Dyer, John M.; Mullen, Robert T.
- Journal of Lipid Research, Vol. 53, Issue 2
Molecular Analysis of a Bifunctional Fatty Acid Conjugase/Desaturase from Tung. Implications for the Evolution of Plant Fatty Acid Diversity
journal, November 2002
- Dyer, John M.; Chapital, Dorselyn C.; Kuan, Jui-Chang W.
- Plant Physiology, Vol. 130, Issue 4
The plant lipidome in human and environmental health
journal, September 2016
- Horn, P. J.; Benning, C.
- Science, Vol. 353, Issue 6305
Oil accumulation in leaves directed by modification of fatty acid breakdown and lipid synthesis pathways
journal, September 2009
- Slocombe, Steve P.; Cornah, Jo; Pinfield-Wells, Helen
- Plant Biotechnology Journal, Vol. 7, Issue 7, p. 694-703
Castor Phospholipid:Diacylglycerol Acyltransferase Facilitates Efficient Metabolism of Hydroxy Fatty Acids in Transgenic Arabidopsis
journal, December 2010
- van Erp, Harrie; Bates, Philip D.; Burgal, Julie
- Plant Physiology, Vol. 155, Issue 2
Purification of a Jojoba Embryo Wax Synthase, Cloning of its cDNA, and Production of High Levels of Wax in Seeds of Transgenic Arabidopsis
journal, March 2000
- Lardizabal, Kathryn D.; Metz, James G.; Sakamoto, Tetsuo
- Plant Physiology, Vol. 122, Issue 3
Tobacco as a production platform for biofuel overexpression of Arabidopsis DGAT and LEC2 genes increases accumulation and shifts the composition of lipids in green biomass
journal, April 2010
- Andrianov, Vyacheslav; Borisjuk, Nikolai; Pogrebnyak, Natalia
- Plant Biotechnology Journal, Vol. 8, Issue 3, p. 277-287
Replacing fossil oil with fresh oil - with what and for what?
journal, April 2011
- Carlsson, Anders S.; Yilmaz, Jenny Lindberg; Green, Allan G.
- European Journal of Lipid Science and Technology, Vol. 113, Issue 7
Desaturation and Related Modifications of Fatty Acids
journal, June 1998
- Shanklin, John; Cahoon, Edgar B.
- Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 49, Issue 1
Singlet Oxygen Is the Major Reactive Oxygen Species Involved in Photooxidative Damage to Plants
journal, August 2008
- Triantaphylidès, Christian; Krischke, Markus; Hoeberichts, Frank Alfons
- Plant Physiology, Vol. 148, Issue 2
Δ12-Oleate Desaturase-related Enzymes Associated with Formation of Conjugated trans -Δ11, cis -Δ13 Double Bonds
journal, December 2002
- Iwabuchi, Mari; Kohno-Murase, Junko; Imamura, Jun
- Journal of Biological Chemistry, Vol. 278, Issue 7
Lipid Droplet-Associated Proteins (LDAPs) Are Required for the Dynamic Regulation of Neutral Lipid Compartmentation in Plant Cells
journal, February 2016
- Gidda, Satinder K.; Park, Sunjung; Pyc, Michal
- Plant Physiology, Vol. 170, Issue 4
Effect of Dietary Tung Oil on the Growth and Lipid Metabolism of Laying Hens.
journal, January 2002
- Lee, Jung-Suck; Takai, Jun-ichi; Takahasi, Kazuaki
- Journal of Nutritional Science and Vitaminology, Vol. 48, Issue 2
Tung Tree DGAT1 and DGAT2 Have Nonredundant Functions in Triacylglycerol Biosynthesis and Are Localized to Different Subdomains of the Endoplasmic Reticulum
journal, August 2006
- Shockey, Jay M.; Gidda, Satinder K.; Chapital, Dorselyn C.
- The Plant Cell, Vol. 18, Issue 9
Accumulation of Ricinoleic, Lesquerolic, and Densipolic Acids in Seeds of Transgenic Arabidopsis Plants That Express a Fatty Acyl Hydroxylase cDNA from Castor Bean
journal, March 1997
- Broun, P.; Somerville, C.
- Plant Physiology, Vol. 113, Issue 3
Altered Lipid Composition and Enhanced Nutritional Value of Arabidopsis Leaves following Introduction of an Algal Diacylglycerol Acyltransferase 2
journal, February 2013
- Sanjaya, ; Miller, Rachel; Durrett, Timothy P.
- The Plant Cell, Vol. 25, Issue 2