Conversion and assimilation of furfural and 5-(hydroxymethyl)furfural by Pseudomonas putida KT2440
Abstract
The sugar dehydration products, furfural and 5-(hydroxymethyl)furfural (HMF), are commonly formed during high-temperature processing of lignocellulose, most often in thermochemical pretreatment, liquefaction, or pyrolysis. Typically, these two aldehydes are considered major inhibitors in microbial conversion processes. Many microbes can convert these compounds to their less toxic, dead-end alcohol counterparts, furfuryl alcohol and 5-(hydroxymethyl)furfuryl alcohol. Recently, the genes responsible for aerobic catabolism of furfural and HMF were discovered in Cupriavidus basilensis HMF14 to enable complete conversion of these compounds to the TCA cycle intermediate, 2-oxo-glutarate. In this work, we engineer the robust soil microbe, Pseudomonas putida KT2440, to utilize furfural and HMF as sole carbon and energy sources via complete genomic integration of the 12 kB hmf gene cluster previously reported from Burkholderia phytofirmans. The common intermediate, 2-furoic acid, is shown to be a bottleneck for both furfural and HMF metabolism. When cultured on biomass hydrolysate containing representative amounts of furfural and HMF from dilute-acid pretreatment, the engineered strain outperforms the wild type microbe in terms of reduced lag time and enhanced growth rates due to catabolism of furfural and HMF. Overall, this study demonstrates that an approach for biological conversion of furfural and HMF, relative to the typical production ofmore »
- Authors:
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office
- OSTI Identifier:
- 1378277
- Alternate Identifier(s):
- OSTI ID: 1346810
- Report Number(s):
- NREL/JA-5100-68119
Journal ID: ISSN 2214-0301; S2214030116300396; PII: S2214030116300396
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Published Article
- Journal Name:
- Metabolic Engineering Communications
- Additional Journal Information:
- Journal Name: Metabolic Engineering Communications Journal Volume: 4 Journal Issue: C; Journal ID: ISSN 2214-0301
- Publisher:
- Elsevier
- Country of Publication:
- Netherlands
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; furfural; 5-(hydroxymethyl)furfural; HMF; pyrolysis; Pseudomonas putida KT2440
Citation Formats
Guarnieri, Michael T., Ann Franden, Mary, Johnson, Christopher W., and Beckham, Gregg T. Conversion and assimilation of furfural and 5-(hydroxymethyl)furfural by Pseudomonas putida KT2440. Netherlands: N. p., 2017.
Web. doi:10.1016/j.meteno.2017.02.001.
Guarnieri, Michael T., Ann Franden, Mary, Johnson, Christopher W., & Beckham, Gregg T. Conversion and assimilation of furfural and 5-(hydroxymethyl)furfural by Pseudomonas putida KT2440. Netherlands. https://doi.org/10.1016/j.meteno.2017.02.001
Guarnieri, Michael T., Ann Franden, Mary, Johnson, Christopher W., and Beckham, Gregg T. Thu .
"Conversion and assimilation of furfural and 5-(hydroxymethyl)furfural by Pseudomonas putida KT2440". Netherlands. https://doi.org/10.1016/j.meteno.2017.02.001.
@article{osti_1378277,
title = {Conversion and assimilation of furfural and 5-(hydroxymethyl)furfural by Pseudomonas putida KT2440},
author = {Guarnieri, Michael T. and Ann Franden, Mary and Johnson, Christopher W. and Beckham, Gregg T.},
abstractNote = {The sugar dehydration products, furfural and 5-(hydroxymethyl)furfural (HMF), are commonly formed during high-temperature processing of lignocellulose, most often in thermochemical pretreatment, liquefaction, or pyrolysis. Typically, these two aldehydes are considered major inhibitors in microbial conversion processes. Many microbes can convert these compounds to their less toxic, dead-end alcohol counterparts, furfuryl alcohol and 5-(hydroxymethyl)furfuryl alcohol. Recently, the genes responsible for aerobic catabolism of furfural and HMF were discovered in Cupriavidus basilensis HMF14 to enable complete conversion of these compounds to the TCA cycle intermediate, 2-oxo-glutarate. In this work, we engineer the robust soil microbe, Pseudomonas putida KT2440, to utilize furfural and HMF as sole carbon and energy sources via complete genomic integration of the 12 kB hmf gene cluster previously reported from Burkholderia phytofirmans. The common intermediate, 2-furoic acid, is shown to be a bottleneck for both furfural and HMF metabolism. When cultured on biomass hydrolysate containing representative amounts of furfural and HMF from dilute-acid pretreatment, the engineered strain outperforms the wild type microbe in terms of reduced lag time and enhanced growth rates due to catabolism of furfural and HMF. Overall, this study demonstrates that an approach for biological conversion of furfural and HMF, relative to the typical production of dead-end alcohols, enables both enhanced carbon conversion and substantially improves tolerance to hydrolysate inhibitors. Furthermore, this approach should find general utility both in emerging aerobic processes for the production of fuels and chemicals from biomass-derived sugars and in the biological conversion of high-temperature biomass streams from liquefaction or pyrolysis where furfural and HMF are much more abundant than in biomass hydrolysates from pretreatment.},
doi = {10.1016/j.meteno.2017.02.001},
journal = {Metabolic Engineering Communications},
number = C,
volume = 4,
place = {Netherlands},
year = {Thu Jun 01 00:00:00 EDT 2017},
month = {Thu Jun 01 00:00:00 EDT 2017}
}
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https://doi.org/10.1016/j.meteno.2017.02.001
https://doi.org/10.1016/j.meteno.2017.02.001
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