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Title: Tepidibacillus decaturensis sp. nov., a microaerophilic, moderately thermophilic iron-reducing bacterium isolated from 1.7 km depth groundwater

Here, a Gram-stain-negative, microaerophilic rod-shaped organism designated as strain Z9 T was isolated from groundwater of 1.7 km depth from the Mt. Simon Sandstone of the Illinois Basin, Illinois, USA. Cells of strain Z9 T were rod shaped with dimensions of 0.3×(1–10) µm and stained Gram-negative. Strain Z9 T grew within the temperature range 20–60 °C (optimum at 30–40 °C), between pH 5 and 8 (optimum 5.2–5.8) and under salt concentrations of 1–5 % (w/v) NaCl (optimum 2.5 % NaCl). In addition to growth by fermentation and nitrate reduction, this strain was able to reduce Fe(III), Mn(IV), Co(III) and Cr(VI) when H 2 or organic carbon was available as the electron donor, but did not actively reduce oxidized sulfur compounds (e.g. sulfate, thiosulfate or S 0). The G+C content of the DNA from strain Z9 T was 36.1 mol%. Phylogenetic analysis of the 16S rRNA gene from strain Z9 T showed that it belongs to the class Bacilli and shares 97 % sequence similarity with the only currently characterized member of the genus Tepidibacillus, T. fermentans. Based on the physiological distinctness and phylogenetic information, strain Z9 T represents a novel species within the genus Tepidibacillus, for which the name Tepidibacillusmore » decaturensis sp. nov. is proposed. The type strain is Z9 T (=ATCC BAA-2644 T=DSM 103037 T).« less
Authors:
 [1] ;  [1] ;  [2] ;  [3] ;  [3] ;  [3] ;  [1] ;  [1] ;  [1] ;  [1]
  1. Univ. of Illinois Urbana-Champaign, Urbana, IL (United States)
  2. Argonne National Lab. (ANL), Argonne, IL (United States); Bulgarian Academy of Sciences, Sofia (Bulgaria)
  3. Argonne National Lab. (ANL), Argonne, IL (United States)
Publication Date:
Grant/Contract Number:
AC02-06CH11357
Type:
Accepted Manuscript
Journal Name:
International Journal of Systematic and Evolutionary Microbiology
Additional Journal Information:
Journal Volume: 66; Journal Issue: 10; Journal ID: ISSN 1466-5026
Publisher:
International Union of Microbiological Societies
Research Org:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); Subsurface Biogeochemical Research; National Energy Technology Laboratory (NETL); National Aeronautic and Space Administration (NASA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Deep subsurface; Firmicutes; Microbial iron reduction; Tepidibacillus
OSTI Identifier:
1346735