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Title: Tepidibacillus decaturensis sp. nov., a microaerophilic, moderately thermophilic iron-reducing bacterium isolated from 1.7 km depth groundwater

Here, a Gram-stain-negative, microaerophilic rod-shaped organism designated as strain Z9 T was isolated from groundwater of 1.7 km depth from the Mt. Simon Sandstone of the Illinois Basin, Illinois, USA. Cells of strain Z9 T were rod shaped with dimensions of 0.3×(1–10) µm and stained Gram-negative. Strain Z9 T grew within the temperature range 20–60 °C (optimum at 30–40 °C), between pH 5 and 8 (optimum 5.2–5.8) and under salt concentrations of 1–5 % (w/v) NaCl (optimum 2.5 % NaCl). In addition to growth by fermentation and nitrate reduction, this strain was able to reduce Fe(III), Mn(IV), Co(III) and Cr(VI) when H 2 or organic carbon was available as the electron donor, but did not actively reduce oxidized sulfur compounds (e.g. sulfate, thiosulfate or S 0). The G+C content of the DNA from strain Z9 T was 36.1 mol%. Phylogenetic analysis of the 16S rRNA gene from strain Z9 T showed that it belongs to the class Bacilli and shares 97 % sequence similarity with the only currently characterized member of the genus Tepidibacillus, T. fermentans. Based on the physiological distinctness and phylogenetic information, strain Z9 T represents a novel species within the genus Tepidibacillus, for which the name Tepidibacillusmore » decaturensis sp. nov. is proposed. The type strain is Z9 T (=ATCC BAA-2644 T=DSM 103037 T).« less
Authors:
 [1] ;  [1] ;  [2] ;  [3] ;  [3] ;  [3] ;  [1] ;  [1] ;  [1] ;  [1]
  1. Univ. of Illinois Urbana-Champaign, Urbana, IL (United States)
  2. Argonne National Lab. (ANL), Argonne, IL (United States); Bulgarian Academy of Sciences, Sofia (Bulgaria)
  3. Argonne National Lab. (ANL), Argonne, IL (United States)
Publication Date:
Grant/Contract Number:
AC02-06CH11357
Type:
Accepted Manuscript
Journal Name:
International Journal of Systematic and Evolutionary Microbiology
Additional Journal Information:
Journal Volume: 66; Journal Issue: 10; Journal ID: ISSN 1466-5026
Publisher:
International Union of Microbiological Societies
Research Org:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); Subsurface Biogeochemical Research; National Energy Technology Laboratory (NETL); National Aeronautic and Space Administration (NASA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Deep subsurface; Firmicutes; Microbial iron reduction; Tepidibacillus
OSTI Identifier:
1346735

Dong, Yiran, Sanford, Robert A., Boyanov, Maxim I., Kemner, Kenneth M., Flynn, Theodore M., O'Loughlin, Edward J., Locke, Randall A., Weber, Joseph R., Egan, Sheila M., and Fouke, Bruce W.. Tepidibacillus decaturensis sp. nov., a microaerophilic, moderately thermophilic iron-reducing bacterium isolated from 1.7 km depth groundwater. United States: N. p., Web. doi:10.1099/ijsem.0.001295.
Dong, Yiran, Sanford, Robert A., Boyanov, Maxim I., Kemner, Kenneth M., Flynn, Theodore M., O'Loughlin, Edward J., Locke, Randall A., Weber, Joseph R., Egan, Sheila M., & Fouke, Bruce W.. Tepidibacillus decaturensis sp. nov., a microaerophilic, moderately thermophilic iron-reducing bacterium isolated from 1.7 km depth groundwater. United States. doi:10.1099/ijsem.0.001295.
Dong, Yiran, Sanford, Robert A., Boyanov, Maxim I., Kemner, Kenneth M., Flynn, Theodore M., O'Loughlin, Edward J., Locke, Randall A., Weber, Joseph R., Egan, Sheila M., and Fouke, Bruce W.. 2016. "Tepidibacillus decaturensis sp. nov., a microaerophilic, moderately thermophilic iron-reducing bacterium isolated from 1.7 km depth groundwater". United States. doi:10.1099/ijsem.0.001295. https://www.osti.gov/servlets/purl/1346735.
@article{osti_1346735,
title = {Tepidibacillus decaturensis sp. nov., a microaerophilic, moderately thermophilic iron-reducing bacterium isolated from 1.7 km depth groundwater},
author = {Dong, Yiran and Sanford, Robert A. and Boyanov, Maxim I. and Kemner, Kenneth M. and Flynn, Theodore M. and O'Loughlin, Edward J. and Locke, Randall A. and Weber, Joseph R. and Egan, Sheila M. and Fouke, Bruce W.},
abstractNote = {Here, a Gram-stain-negative, microaerophilic rod-shaped organism designated as strain Z9T was isolated from groundwater of 1.7 km depth from the Mt. Simon Sandstone of the Illinois Basin, Illinois, USA. Cells of strain Z9T were rod shaped with dimensions of 0.3×(1–10) µm and stained Gram-negative. Strain Z9T grew within the temperature range 20–60 °C (optimum at 30–40 °C), between pH 5 and 8 (optimum 5.2–5.8) and under salt concentrations of 1–5 % (w/v) NaCl (optimum 2.5 % NaCl). In addition to growth by fermentation and nitrate reduction, this strain was able to reduce Fe(III), Mn(IV), Co(III) and Cr(VI) when H2 or organic carbon was available as the electron donor, but did not actively reduce oxidized sulfur compounds (e.g. sulfate, thiosulfate or S0). The G+C content of the DNA from strain Z9T was 36.1 mol%. Phylogenetic analysis of the 16S rRNA gene from strain Z9T showed that it belongs to the class Bacilli and shares 97 % sequence similarity with the only currently characterized member of the genus Tepidibacillus, T. fermentans. Based on the physiological distinctness and phylogenetic information, strain Z9T represents a novel species within the genus Tepidibacillus, for which the name Tepidibacillus decaturensis sp. nov. is proposed. The type strain is Z9T (=ATCC BAA-2644T=DSM 103037T).},
doi = {10.1099/ijsem.0.001295},
journal = {International Journal of Systematic and Evolutionary Microbiology},
number = 10,
volume = 66,
place = {United States},
year = {2016},
month = {10}
}