Detection of phosphate transporter genes from arbuscular mycorrhizal fungi in mature tree roots under experimental soil pH manipulation
Abstract
We present the majority of terrestrial plant roots are colonized by arbuscular mycorrhizal (AM) fungi that, in exchange for carbon, provide plants with enhanced nutrient uptake — most notably inorganic phosphate (Pi). To mediate the uptake of Pi from the soil, AM fungi possess high affinity inorganic phosphate transporters (PTs). Under laboratory conditions, Pi concentrations have been shown to regulate AM fungal-specific PT gene expression. The relationship between PT expression and Pi in the field remains unexplored. Here we quantify AM fungal-specific PTs from maple tree roots in situ. In an effort to limit edaphic parameters, root samples were collected from manipulated forested plots that had elevated soil Pi availability, either through direct Pi application or elevating pH to lower exchangeable aluminum. The aim of the study was to examine AM fungal-specific PT gene expression both prior to and following soil Pi amendment; however, a direct correlation between soil Pi concentration and PT gene expression was not observed. PT transcripts were detected to a greater extent under elevated pH and, while our results are confounded by an overall low detection of PT genes (23 % of all samples collected), our findings raise interesting questions regarding the role of soil pHmore »
- Authors:
-
- Holden Arboretum, Kirtland (United States); Case Western Reserve Univ., Cleveland, OH (United States). Dept. of Biology
- Holden Arboretum, Kirtland (United States); Case Western Reserve Univ., Cleveland, OH (United States). Dept. of Biology; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
- Case Western Reserve Univ., Cleveland, OH (United States). Dept. of Biology; North Carolina State Univ., Raleigh, NC (United States). Dept. of Biological Sciences
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1346660
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Symbiosis
- Additional Journal Information:
- Journal Volume: 72; Journal Issue: 2; Journal ID: ISSN 0334-5114
- Publisher:
- Springer
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; AM fungi; Inorganic phosphorus; Quantitative PCR
Citation Formats
Carrino-Kyker, Sarah R., Kluber, Laurel A., Coyle, Kaitlin P., and Burke, David J. Detection of phosphate transporter genes from arbuscular mycorrhizal fungi in mature tree roots under experimental soil pH manipulation. United States: N. p., 2016.
Web. doi:10.1007/s13199-016-0448-1.
Carrino-Kyker, Sarah R., Kluber, Laurel A., Coyle, Kaitlin P., & Burke, David J. Detection of phosphate transporter genes from arbuscular mycorrhizal fungi in mature tree roots under experimental soil pH manipulation. United States. https://doi.org/10.1007/s13199-016-0448-1
Carrino-Kyker, Sarah R., Kluber, Laurel A., Coyle, Kaitlin P., and Burke, David J. Tue .
"Detection of phosphate transporter genes from arbuscular mycorrhizal fungi in mature tree roots under experimental soil pH manipulation". United States. https://doi.org/10.1007/s13199-016-0448-1. https://www.osti.gov/servlets/purl/1346660.
@article{osti_1346660,
title = {Detection of phosphate transporter genes from arbuscular mycorrhizal fungi in mature tree roots under experimental soil pH manipulation},
author = {Carrino-Kyker, Sarah R. and Kluber, Laurel A. and Coyle, Kaitlin P. and Burke, David J.},
abstractNote = {We present the majority of terrestrial plant roots are colonized by arbuscular mycorrhizal (AM) fungi that, in exchange for carbon, provide plants with enhanced nutrient uptake — most notably inorganic phosphate (Pi). To mediate the uptake of Pi from the soil, AM fungi possess high affinity inorganic phosphate transporters (PTs). Under laboratory conditions, Pi concentrations have been shown to regulate AM fungal-specific PT gene expression. The relationship between PT expression and Pi in the field remains unexplored. Here we quantify AM fungal-specific PTs from maple tree roots in situ. In an effort to limit edaphic parameters, root samples were collected from manipulated forested plots that had elevated soil Pi availability, either through direct Pi application or elevating pH to lower exchangeable aluminum. The aim of the study was to examine AM fungal-specific PT gene expression both prior to and following soil Pi amendment; however, a direct correlation between soil Pi concentration and PT gene expression was not observed. PT transcripts were detected to a greater extent under elevated pH and, while our results are confounded by an overall low detection of PT genes (23 % of all samples collected), our findings raise interesting questions regarding the role of soil pH on PT function. In conclusion, our study is a first step in understanding how edaphic properties influence PT expression and plant P acquisition in mature tree roots.},
doi = {10.1007/s13199-016-0448-1},
journal = {Symbiosis},
number = 2,
volume = 72,
place = {United States},
year = {Tue Oct 04 00:00:00 EDT 2016},
month = {Tue Oct 04 00:00:00 EDT 2016}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 4 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Mycorrhizal fungal communities respond to experimental elevation of soil pH and P availability in temperate hardwood forests
journal, February 2016
- Carrino-Kyker, Sarah R.; Kluber, Laurel A.; Petersen, Sheryl M.
- FEMS Microbiology Ecology, Vol. 92, Issue 3
Mycorrhizal Fungi Can Dominate Phosphate Supply to Plants Irrespective of Growth Responses
journal, September 2003
- Smith, Sally E.; Smith, F. Andrew; Jakobsen, Iver
- Plant Physiology, Vol. 133, Issue 1
A phosphate transporter from the mycorrhizal fungus Glomus versiforme
journal, December 1995
- Harrison, Maria J.; Buuren, Marianne L. van
- Nature, Vol. 378, Issue 6557
The transcriptome of the arbuscular mycorrhizal fungus Glomus intraradices (DAOM 197198) reveals functional tradeoffs in an obligate symbiont
journal, November 2011
- Tisserant, E.; Kohler, A.; Dozolme-Seddas, P.
- New Phytologist, Vol. 193, Issue 3
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
journal, January 1994
- Thompson, Julie D.; Higgins, Desmond G.; Gibson, Toby J.
- Nucleic Acids Research, Vol. 22, Issue 22, p. 4673-4680
Symbiotic phosphate transport in arbuscular mycorrhizas
journal, January 2005
- Karandashov, Vladimir; Bucher, Marcel
- Trends in Plant Science, Vol. 10, Issue 1
Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities
journal, October 2009
- Schloss, P. D.; Westcott, S. L.; Ryabin, T.
- Applied and Environmental Microbiology, Vol. 75, Issue 23, p. 7537-7541
Mycorrhizal Response to Experimental pH and P Manipulation in Acidic Hardwood Forests
journal, November 2012
- Kluber, Laurel A.; Carrino-Kyker, Sarah R.; Coyle, Kaitlin P.
- PLoS ONE, Vol. 7, Issue 11
Reference genes in real-time PCR
journal, September 2013
- Kozera, Bartłomiej; Rapacz, Marcin
- Journal of Applied Genetics, Vol. 54, Issue 4
A Phosphate Transporter Gene from the Extra-Radical Mycelium of an Arbuscular Mycorrhizal Fungus Glomus intraradices Is Regulated in Response to Phosphate in the Environment
journal, October 2001
- Maldonado-Mendoza, Ignacio E.; Dewbre, Gary R.; Harrison, Maria J.
- Molecular Plant-Microbe Interactions®, Vol. 14, Issue 10
Phosphorus nutrition of mycorrhizal trees
journal, July 2010
- Plassard, C.; Dell, B.
- Tree Physiology, Vol. 30, Issue 9
Ploughing up the wood-wide web?
journal, July 1998
- Helgason, T.; Daniell, T. J.; Husband, R.
- Nature, Vol. 394, Issue 6692
Functional biology of plant phosphate uptake at root and mycorrhiza interfaces: Tansley review
journal, December 2006
- Bucher, Marcel
- New Phytologist, Vol. 173, Issue 1
Effects of Alliaria petiolata (garlic mustard; Brassicaceae) on mycorrhizal colonization and community structure in three herbaceous plants in a mixed deciduous forest
journal, October 2008
- Burke, D. J.
- American Journal of Botany, Vol. 95, Issue 11
Fine root Architecture of nine North American Trees
journal, May 2002
- Pregitzer, Kurt S.; DeForest, Jared L.; Burton, Andrew J.
- Ecological Monographs, Vol. 72, Issue 2
Phosphate in the arbuscular mycorrhizal symbiosis: transport properties and regulatory roles: Phosphate transport in the AM symbiosis
journal, December 2006
- Javot, HÉLÈNe; Pumplin, Nathan; Harrison, Maria J.
- Plant, Cell & Environment, Vol. 30, Issue 3
The relative contribution of hyphae and roots to phosphorus uptake by arbuscular mycorrhizal plants, measured by dual labelling with 32P and 33P
journal, July 1993
- Pearson, J. N.; Jakobsen, I.
- New Phytologist, Vol. 124, Issue 3
Seasonal variation in mycorrhizal fungi colonizing roots of Allium tricoccum (wild leek) in a mature mixed hardwood forest
journal, January 2015
- Hewins, Charlotte R.; Carrino-Kyker, Sarah R.; Burke, David J.
- Mycorrhiza, Vol. 25, Issue 6
Soil microbial responses to elevated phosphorus and pH in acidic temperate deciduous forests
journal, July 2011
- DeForest, Jared L.; Smemo, Kurt A.; Burke, David J.
- Biogeochemistry, Vol. 109, Issue 1-3
Long-Term Effects of Acid Rain: Response and Recovery of a Forest Ecosystem
journal, April 1996
- Likens, G. E.; Driscoll, C. T.; Buso, D. C.
- Science, Vol. 272, Issue 5259
Revealing Natural Relationships among Arbuscular Mycorrhizal Fungi: Culture Line BEG47 Represents Diversispora epigaea, Not Glomus versiforme
journal, August 2011
- Schüßler, Arthur; Krüger, Manuela; Walker, Christopher
- PLoS ONE, Vol. 6, Issue 8