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Title: A Reversibly Sealed, Easy Access, Modular (SEAM) Microfluidic Architecture to Establish In Vitro Tissue Interfaces

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Publication Date:
Research Org.:
Sandia National Lab. (SNL-CA), Livermore, CA (United States); Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
OSTI Identifier:
Alternate Identifier(s):
OSTI ID: 1266214
Grant/Contract Number:  
10027IA-3167; AC04-94AL85000
Resource Type:
Published Article
Journal Name:
Additional Journal Information:
Journal Name: PLoS ONE Journal Volume: 11 Journal Issue: 5; Journal ID: ISSN 1932-6203
Public Library of Science (PLoS)
Country of Publication:
United States
59 BASIC BIOLOGICAL SCIENCES; microfluidics; RNA extraction; gene expression; lasers; cell cultures; fluidics; RNA isolation; membrane staining

Citation Formats

Abhyankar, Vinay V., Wu, Meiye, Koh, Chung-Yan, Hatch, Anson V., and Eddington, ed., David T. A Reversibly Sealed, Easy Access, Modular (SEAM) Microfluidic Architecture to Establish In Vitro Tissue Interfaces. United States: N. p., 2016. Web. doi:10.1371/journal.pone.0156341.
Abhyankar, Vinay V., Wu, Meiye, Koh, Chung-Yan, Hatch, Anson V., & Eddington, ed., David T. A Reversibly Sealed, Easy Access, Modular (SEAM) Microfluidic Architecture to Establish In Vitro Tissue Interfaces. United States. doi:10.1371/journal.pone.0156341.
Abhyankar, Vinay V., Wu, Meiye, Koh, Chung-Yan, Hatch, Anson V., and Eddington, ed., David T. Thu . "A Reversibly Sealed, Easy Access, Modular (SEAM) Microfluidic Architecture to Establish In Vitro Tissue Interfaces". United States. doi:10.1371/journal.pone.0156341.
title = {A Reversibly Sealed, Easy Access, Modular (SEAM) Microfluidic Architecture to Establish In Vitro Tissue Interfaces},
author = {Abhyankar, Vinay V. and Wu, Meiye and Koh, Chung-Yan and Hatch, Anson V. and Eddington, ed., David T.},
abstractNote = {},
doi = {10.1371/journal.pone.0156341},
journal = {PLoS ONE},
number = 5,
volume = 11,
place = {United States},
year = {2016},
month = {5}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1371/journal.pone.0156341

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Works referenced in this record:

Physical properties of hierarchically ordered self-assembled planar and spherical membranes
journal, January 2010

  • Carvajal, Daniel; Bitton, Ronit; Mantei, Jason R.
  • Soft Matter, Vol. 6, Issue 8
  • DOI: 10.1039/b923903k

Extracellular matrix as a biological scaffold material: Structure and function
journal, January 2009

Advanced Material Strategies for Tissue Engineering Scaffolds
journal, September 2009

  • Freed, Lisa E.; Engelmayr, George C.; Borenstein, Jeffrey T.
  • Advanced Materials, Vol. 21, Issue 32-33
  • DOI: 10.1002/adma.200900303

Development of hyaluronic acid-based scaffolds for brain tissue engineering
journal, September 2009

Microengineered physiological biomimicry: Organs-on-Chips
journal, January 2012

  • Huh, Dongeun; Torisawa, Yu-suke; Hamilton, Geraldine A.
  • Lab on a Chip, Vol. 12, Issue 12
  • DOI: 10.1039/c2lc40089h

Self-Assembly and Mineralization of Peptide-Amphiphile Nanofibers
journal, November 2001

  • Hartgerink, Jeffrey D.; Beniash, Elia; Stupp, Samuel I.
  • Science, Vol. 294, Issue 5547, p. 1684-1688
  • DOI: 10.1126/science.1063187

Microfluidic tectonics: A comprehensive construction platform for microfluidic systems
journal, November 2000

  • Beebe, D. J.; Moore, J. S.; Yu, Q.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 25, p. 13488-13493
  • DOI: 10.1073/pnas.250273097

Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments
journal, September 2004

  • Engler, Adam J.; Griffin, Maureen A.; Sen, Shamik
  • The Journal of Cell Biology, Vol. 166, Issue 6
  • DOI: 10.1083/jcb.200405004

The pressure drop along rectangular microchannels containing bubbles
journal, January 2007

  • Fuerstman, Michael J.; Lai, Ann; Thurlow, Meghan E.
  • Lab on a Chip, Vol. 7, Issue 11
  • DOI: 10.1039/b706549c

c-fos regulates neuronal excitability and survival
journal, March 2002

  • Zhang, Jianhua; Zhang, Dongsheng; McQuade, Jill Slane
  • Nature Genetics, Vol. 30, Issue 4
  • DOI: 10.1038/ng859

Lab-on-a-chip devices: How to close and plug the lab?
journal, January 2015

Arrays of horizontally-oriented mini-reservoirs generate steady microfluidic flows for continuous perfusion cell culture and gradient generation
journal, January 2004

  • Zhu, Xiaoyue; Yi Chu, Leonard; Chueh, Bor-han
  • The Analyst, Vol. 129, Issue 11
  • DOI: 10.1039/b407623k

Microfluidic Arrays of Fluid−Fluid Diffusional Contacts as Detection Elements and Combinatorial Tools
journal, November 2001

  • Ismagilov, Rustem F.; Ng, Jessamine M. K.; Kenis, Paul J. A.
  • Analytical Chemistry, Vol. 73, Issue 21
  • DOI: 10.1021/ac010502a

A microfluidic device for a pharmacokinetic–pharmacodynamic (PK–PD) model on a chip
journal, January 2010

  • Sung, Jong Hwan; Kam, Carrie; Shuler, Michael L.
  • Lab on a Chip, Vol. 10, Issue 4
  • DOI: 10.1039/b917763a

Astrocyte–endothelial interactions at the blood–brain barrier
journal, January 2006

  • Abbott, N. Joan; Rönnbäck, Lars; Hansson, Elisabeth
  • Nature Reviews Neuroscience, Vol. 7, Issue 1
  • DOI: 10.1038/nrn1824

Reconstituting Organ-Level Lung Functions on a Chip
journal, June 2010

On chip porous polymer membranes for integration of gastrointestinal tract epithelium with microfluidic ‘body-on-a-chip’ devices
journal, July 2012

  • Esch, Mandy Brigitte; Sung, Jong Hwan; Yang, Jennifer
  • Biomedical Microdevices, Vol. 14, Issue 5
  • DOI: 10.1007/s10544-012-9669-0

Pumpless microfluidic platform for drug testing on human skin equivalents
journal, January 2015

  • Abaci, Hasan Erbil; Gledhill, Karl; Guo, Zongyou
  • Lab on a Chip, Vol. 15, Issue 3
  • DOI: 10.1039/C4LC00999A

Substrate Stiffness Regulates PDGF-Induced Circular Dorsal Ruffle Formation Through MLCK
journal, May 2013

  • Huynh, John; Bordeleau, Francois; Kraning-Rush, Casey M.
  • Cellular and Molecular Bioengineering, Vol. 6, Issue 2
  • DOI: 10.1007/s12195-013-0278-7

Large-Scale, Ultrapliable, and Free-Standing Nanomembranes
journal, February 2013

Molecular mechanisms of mechanotransduction in mammalian sensory neurons
journal, February 2011

  • Delmas, Patrick; Hao, Jizhe; Rodat-Despoix, Lise
  • Nature Reviews Neuroscience, Vol. 12, Issue 3
  • DOI: 10.1038/nrn2993

RNA processing and its regulation: global insights into biological networks
journal, January 2010

  • Licatalosi, Donny D.; Darnell, Robert B.
  • Nature Reviews Genetics, Vol. 11, Issue 1
  • DOI: 10.1038/nrg2673

Clear castable polyurethane elastomer for fabrication of microfluidic devices
journal, January 2013

  • Domansky, Karel; Leslie, Daniel C.; McKinney, James
  • Lab on a Chip, Vol. 13, Issue 19
  • DOI: 10.1039/c3lc50558h

A review of microfabrication and hydrogel engineering for micro-organs on chips
journal, February 2014

Streamlining gene expression analysis: integration of co-culture and mRNA purification
journal, January 2014

  • Berry, Scott M.; Singh, Chandresh; Lang, Jessica D.
  • Integrative Biology, Vol. 6, Issue 2
  • DOI: 10.1039/c3ib40136g

Microfabrication of human organs-on-chips
journal, October 2013

  • Huh, Dongeun; Kim, Hyun Jung; Fraser, Jacob P.
  • Nature Protocols, Vol. 8, Issue 11
  • DOI: 10.1038/nprot.2013.137

Multi-cellular 3D human primary liver cell culture elevates metabolic activity under fluidic flow
journal, January 2015

  • Esch, Mandy B.; Prot, Jean-Matthieu; Wang, Ying I.
  • Lab on a Chip, Vol. 15, Issue 10
  • DOI: 10.1039/C5LC00237K

Human gut-on-a-chip inhabited by microbial flora that experiences intestinal peristalsis-like motions and flow
journal, January 2012

  • Kim, Hyun Jung; Huh, Dongeun; Hamilton, Geraldine
  • Lab on a Chip, Vol. 12, Issue 12
  • DOI: 10.1039/c2lc40074j

Thin collagen film scaffolds for retinal epithelial cell culture
journal, March 2007

How multi-organ microdevices can help foster drug development
journal, April 2014

  • Esch, Mandy B.; Smith, Alec S. T.; Prot, Jean-Matthieu
  • Advanced Drug Delivery Reviews, Vol. 69-70
  • DOI: 10.1016/j.addr.2013.12.003

Peptide-amphiphile nanofibers: A versatile scaffold for the preparation of self-assembling materials
journal, April 2002

  • Hartgerink, J. D.; Beniash, E.; Stupp, S. I.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 8, p. 5133-5138
  • DOI: 10.1073/pnas.072699999

A bioactive self-assembled membrane to promote angiogenesis
journal, February 2011

The elastic modulus of Matrigel™ as determined by atomic force microscopy
journal, September 2009

  • Soofi, Shauheen S.; Last, Julie A.; Liliensiek, Sara J.
  • Journal of Structural Biology, Vol. 167, Issue 3
  • DOI: 10.1016/j.jsb.2009.05.005

PDMS absorption of small molecules and consequences in microfluidic applications
journal, January 2006

  • Toepke, Michael W.; Beebe, David J.
  • Lab on a Chip, Vol. 6, Issue 12
  • DOI: 10.1039/b612140c

A Cell Culture System for the Structure and Hydrogel Properties of Basement Membranes: Application to Capillary Walls
journal, February 2012

  • Bruggeman, Leslie A.; Doan, Ryan P.; Loftis, Jacqueline
  • Cellular and Molecular Bioengineering, Vol. 5, Issue 2
  • DOI: 10.1007/s12195-012-0221-3

From 3D cell culture to organs-on-chips
journal, December 2011

  • Huh, Dongeun; Hamilton, Geraldine A.; Ingber, Donald E.
  • Trends in Cell Biology, Vol. 21, Issue 12
  • DOI: 10.1016/j.tcb.2011.09.005

Elasticity of Native and Cross-Linked Polyelectrolyte Multilayer Films
journal, September 2004

  • Richert, Ludovic; Engler, Adam J.; Discher, Dennis E.
  • Biomacromolecules, Vol. 5, Issue 5
  • DOI: 10.1021/bm0498023

Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane)
journal, October 1998

  • Duffy, David C.; McDonald, J. Cooper; Schueller, Olivier J. A.
  • Analytical Chemistry, Vol. 70, Issue 23, p. 4974-4984
  • DOI: 10.1021/ac980656z

Molecular mechanisms of NCAM function
journal, January 2004

  • Hinsby, Anders, M.
  • Frontiers in Bioscience, Vol. 9, Issue 1-3
  • DOI: 10.2741/1393

Using transcriptomics to guide lead optimization in drug discovery projects: Lessons learned from the QSTAR project
journal, May 2015

Generation of Human Pulmonary Microvascular Endothelial Cell Lines
journal, December 2001

  • Krump-Konvalinkova, Vera; Bittinger, Fernando; Unger, Ronald E.
  • Laboratory Investigation, Vol. 81, Issue 12
  • DOI: 10.1038/labinvest.3780385

Integrated Preconcentration SDS−PAGE of Proteins in Microchips Using Photopatterned Cross-Linked Polyacrylamide Gels
journal, July 2006

  • Hatch, Anson V.; Herr, Amy E.; Throckmorton, Daniel J.
  • Analytical Chemistry, Vol. 78, Issue 14, p. 4976-4984
  • DOI: 10.1021/ac0600454

Topographically-patterned porous membranes in a microfluidic device as an in vitro model of renal reabsorptive barriers
journal, January 2013

  • Frohlich, Else M.; Alonso, José Luis; Borenstein, Jeffrey T.
  • Lab on a Chip, Vol. 13, Issue 12
  • DOI: 10.1039/c3lc50199j

Innate immunity and pulmonary host defense
journal, February 2000

Microfluidic immunoassays as rapid saliva-based clinical diagnostics
journal, March 2007

  • Herr, A. E.; Hatch, A. V.; Throckmorton, D. J.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 13, p. 5268-5273
  • DOI: 10.1073/pnas.0607254104

A Theoretical Model Study of the Influence of Fluid Stresses on a Cell Adhering to a Microchannel Wall
journal, August 1998

Brain extracellular matrix
journal, January 1996

Cell responses to the mechanochemical microenvironment—Implications for regenerative medicine and drug delivery☆
journal, November 2007

Biomimetic tissues on a chip for drug discovery
journal, February 2012

Endothelium-derived Toll-like receptor-4 is the key molecule in LPS-induced neutrophil sequestration into lungs
journal, April 2003

  • Andonegui, Graciela; Bonder, Claudine S.; Green, Francis
  • Journal of Clinical Investigation, Vol. 111, Issue 7
  • DOI: 10.1172/JCI16510

Regulation and Repair of the Alveolar-Capillary Barrier in Acute Lung Injury
journal, February 2013

Microfabricated Modular Scale-Down Device for Regenerative Medicine Process Development
journal, December 2012

Quantitative Analysis of Molecular Absorption into PDMS Microfluidic Channels
journal, April 2012

  • Wang, Jack D.; Douville, Nicholas J.; Takayama, Shuichi
  • Annals of Biomedical Engineering, Vol. 40, Issue 9
  • DOI: 10.1007/s10439-012-0562-z

Lift-off of large-scale ultrathin nanomembranes
journal, December 2014

  • Miller, Joshua J.; Carter, Robert N.; McNabb, Kelly B.
  • Journal of Micromechanics and Microengineering, Vol. 25, Issue 1
  • DOI: 10.1088/0960-1317/25/1/015011

Microfluidic organs-on-chips
journal, August 2014

  • Bhatia, Sangeeta N.; Ingber, Donald E.
  • Nature Biotechnology, Vol. 32, Issue 8
  • DOI: 10.1038/nbt.2989

Self-Assembly of Large and Small Molecules into Hierarchically Ordered Sacs and Membranes
journal, March 2008

  • Capito, R. M.; Azevedo, H. S.; Velichko, Y. S.
  • Science, Vol. 319, Issue 5871, p. 1812-1816
  • DOI: 10.1126/science.1154586

On Trk for Retrograde Signaling
journal, December 2001

Microfluidic Immunoassays
journal, June 2010

  • Lin, Chun-Che; Wang, Jung-Hao; Wu, Hui-Wen
  • Journal of the Association for Laboratory Automation, Vol. 15, Issue 3
  • DOI: 10.1016/j.jala.2010.01.013

Self-assembling peptide amphiphile nanofiber matrices for cell entrapment
journal, July 2005

    Works referencing / citing this record:

    Ultrathin transparent membranes for cellular barrier and co-culture models
    journal, February 2017

    • Carter, Robert N.; Casillo, Stephanie M.; Mazzocchi, Andrea R.
    • Biofabrication, Vol. 9, Issue 1
    • DOI: 10.1088/1758-5090/aa5ba7