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Title: Development and characterization of microsatellite loci for the endangered scrub Lupine, Lupinus aridorum (Fabaceae)

Microsatellite primers were developed in scrub lupine ( Lupinus aridorum, Fabaceae), an endemic species to Florida that is listed as endangered in the United States, to assess connectivity among populations, identify hybrids, and examine genetic diversity. We isolated and characterized 12 microsatellite loci polymorphic in scrub lupine or in closely related species (i.e., sky-blue lupine [ L. diffusus] and Gulf Coast lupine [ L. westianus]). Loci showed low to moderate polymorphism, ranging from two to 14 alleles per locus and 0.01 to 0.86 observed heterozygosity. In conclusion, these loci are the first developed for Florida species of lupine and will be used to determine differentiation among species and to aid in conservation of the endangered scrub lupine.
Authors:
 [1] ;  [2] ;  [2] ;  [3] ;  [3] ;  [1]
  1. Florida Institute of Technology, Melbourne, FL (United States)
  2. Bok Tower Gardens, Lake Wales, FL (United States)
  3. Univ. of Georgia, Aiken, SC (United States)
Publication Date:
Grant/Contract Number:
FC09-07SR22506
Type:
Accepted Manuscript
Journal Name:
Applications in Plant Science
Additional Journal Information:
Journal Volume: 3; Journal Issue: 4; Journal ID: ISSN 2168-0450
Publisher:
Botanical Society of America
Research Org:
Univ. of Georgia Research Foundation, Inc., Athens, GA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Fabaceae; Florida; Lupinus; microsatellite; PCR primers
OSTI Identifier:
1345829