Interactions of 1D- and 2D-layered inorganic nanoparticles with fibroblasts and human mesenchymal stem cells
Abstract
Here, this study investigates the effects of tungsten disulfide nanotubes (WSNTs) and molybdenum disulfide nanoplatelets (MSNPs) on fibroblasts (NIH-3T3) and mesenchymal stem cells (MSCs) to determine safe dosages for potential biomedical applications. Cytotoxicity of MSNPs and WSNTs (5–300 μg/ml) on NIH-3T3 and MSCs was assessed at 6, 12 or 24 h. MSC differentiation to adipocytes and osteoblasts was assessed following treatment for 24 h. Only NIH-3T3 cells treated with MSNPs showed dose or time dependent increase in cytotoxicity. Differentiation markers of MSCs in treated groups were unaffected compared with untreated controls. In conclusion, MSNPs and WSNTs at concentrations less than 50 μg/ml are potentially safe for treatment of fibroblasts or MSCs for up to 24 h.
- Authors:
-
- Stony Brook Univ., Stony Brook, NY (United States). Dept. of Biomedical Engineering
- Publication Date:
- Research Org.:
- Stony Brook Univ., NY (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1345589
- Grant/Contract Number:
- AC02-98CH10886; 1DP2OD007394-01
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nanomedicine
- Additional Journal Information:
- Journal Volume: 10; Journal Issue: 11; Journal ID: ISSN 1743-5889
- Publisher:
- Future Medicine
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 77 NANOSCIENCE AND NANOTECHNOLOGY; 59 BASIC BIOLOGICAL SCIENCES; cytotoxicity; differentiation; inorganic nanoparticles; stem cells; tissue engineering; uptake
Citation Formats
Rashkow, Jason Thomas, Talukdar, Yahfi, Lalwani, Gaurav, and Sitharaman, Balaji. Interactions of 1D- and 2D-layered inorganic nanoparticles with fibroblasts and human mesenchymal stem cells. United States: N. p., 2015.
Web. doi:10.2217/nnm.15.35.
Rashkow, Jason Thomas, Talukdar, Yahfi, Lalwani, Gaurav, & Sitharaman, Balaji. Interactions of 1D- and 2D-layered inorganic nanoparticles with fibroblasts and human mesenchymal stem cells. United States. https://doi.org/10.2217/nnm.15.35
Rashkow, Jason Thomas, Talukdar, Yahfi, Lalwani, Gaurav, and Sitharaman, Balaji. Mon .
"Interactions of 1D- and 2D-layered inorganic nanoparticles with fibroblasts and human mesenchymal stem cells". United States. https://doi.org/10.2217/nnm.15.35. https://www.osti.gov/servlets/purl/1345589.
@article{osti_1345589,
title = {Interactions of 1D- and 2D-layered inorganic nanoparticles with fibroblasts and human mesenchymal stem cells},
author = {Rashkow, Jason Thomas and Talukdar, Yahfi and Lalwani, Gaurav and Sitharaman, Balaji},
abstractNote = {Here, this study investigates the effects of tungsten disulfide nanotubes (WSNTs) and molybdenum disulfide nanoplatelets (MSNPs) on fibroblasts (NIH-3T3) and mesenchymal stem cells (MSCs) to determine safe dosages for potential biomedical applications. Cytotoxicity of MSNPs and WSNTs (5–300 μg/ml) on NIH-3T3 and MSCs was assessed at 6, 12 or 24 h. MSC differentiation to adipocytes and osteoblasts was assessed following treatment for 24 h. Only NIH-3T3 cells treated with MSNPs showed dose or time dependent increase in cytotoxicity. Differentiation markers of MSCs in treated groups were unaffected compared with untreated controls. In conclusion, MSNPs and WSNTs at concentrations less than 50 μg/ml are potentially safe for treatment of fibroblasts or MSCs for up to 24 h.},
doi = {10.2217/nnm.15.35},
journal = {Nanomedicine},
number = 11,
volume = 10,
place = {United States},
year = {Mon Jun 01 00:00:00 EDT 2015},
month = {Mon Jun 01 00:00:00 EDT 2015}
}
Web of Science
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