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Title: Ubiquitin facilitates a quality-control pathway that removes damaged chloroplasts

Abstract

Energy production by chloroplasts and mitochondria causes constant oxidative damage. A functioning photosynthetic cell requires quality-control mechanisms to turn over and degrade chloroplasts damaged by reactive oxygen species (ROS). Here in this study, we generated a conditionally lethal Arabidopsis mutant that accumulated excess protoporphyrin IX in the chloroplast and produced singlet oxygen. Damaged chloroplasts were subsequently ubiquitinated and selectively degraded. A genetic screen identified the plant U-box 4 (PUB4) E3 ubiquitin ligase as being necessary for this process. pub4-6 mutants had defects in stress adaptation and longevity. As a result, we have identified a signal that leads to the targeted removal of ROS-overproducing chloroplasts.

Authors:
 [1];  [2];  [3];  [4];  [5];  [6];  [2];  [1]
  1. The Salk Inst., La Jolla, CA (United States)
  2. The Salk Inst., La Jolla, CA (United States); Washington Univ. School of Medicine, St. Louis, MO (United States)
  3. The Salk Inst., La Jolla, CA (United States); Univ. of California-San Diego, La Jolla, CA (United States)
  4. The Salk Inst., La Jolla, CA (United States); Shepherd Univ., Shepherdstown, WV (United States)
  5. Max Planck Institute for Developmental Biology, Tubingen (Germany); Univ. of California, Los Angeles, CA (United States)
  6. Max Planck Institute for Developmental Biology, Tubingen (Germany)
Publication Date:
Research Org.:
The Salk Inst., La Jolla, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Institutes of Health (NIH); German Research Foundation (DFG)
OSTI Identifier:
1345047
Grant/Contract Number:  
FG02-04ER15540; CA014195; NS072031
Resource Type:
Accepted Manuscript
Journal Name:
Science
Additional Journal Information:
Journal Volume: 350; Journal Issue: 6259; Journal ID: ISSN 0036-8075
Publisher:
AAAS
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Woodson, Jesse D., Joens, Matthew S., Sinson, Andrew B., Gilkerson, Jonathan, Salome, Patrice A., Weigel, Detlef, Fitzpatrick, James A., and Chory, Joanne. Ubiquitin facilitates a quality-control pathway that removes damaged chloroplasts. United States: N. p., 2015. Web. doi:10.1126/science.aac7444.
Woodson, Jesse D., Joens, Matthew S., Sinson, Andrew B., Gilkerson, Jonathan, Salome, Patrice A., Weigel, Detlef, Fitzpatrick, James A., & Chory, Joanne. Ubiquitin facilitates a quality-control pathway that removes damaged chloroplasts. United States. doi:10.1126/science.aac7444.
Woodson, Jesse D., Joens, Matthew S., Sinson, Andrew B., Gilkerson, Jonathan, Salome, Patrice A., Weigel, Detlef, Fitzpatrick, James A., and Chory, Joanne. Fri . "Ubiquitin facilitates a quality-control pathway that removes damaged chloroplasts". United States. doi:10.1126/science.aac7444. https://www.osti.gov/servlets/purl/1345047.
@article{osti_1345047,
title = {Ubiquitin facilitates a quality-control pathway that removes damaged chloroplasts},
author = {Woodson, Jesse D. and Joens, Matthew S. and Sinson, Andrew B. and Gilkerson, Jonathan and Salome, Patrice A. and Weigel, Detlef and Fitzpatrick, James A. and Chory, Joanne},
abstractNote = {Energy production by chloroplasts and mitochondria causes constant oxidative damage. A functioning photosynthetic cell requires quality-control mechanisms to turn over and degrade chloroplasts damaged by reactive oxygen species (ROS). Here in this study, we generated a conditionally lethal Arabidopsis mutant that accumulated excess protoporphyrin IX in the chloroplast and produced singlet oxygen. Damaged chloroplasts were subsequently ubiquitinated and selectively degraded. A genetic screen identified the plant U-box 4 (PUB4) E3 ubiquitin ligase as being necessary for this process. pub4-6 mutants had defects in stress adaptation and longevity. As a result, we have identified a signal that leads to the targeted removal of ROS-overproducing chloroplasts.},
doi = {10.1126/science.aac7444},
journal = {Science},
number = 6259,
volume = 350,
place = {United States},
year = {2015},
month = {10}
}

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Cited by: 21 works
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