Mapping mechanical force propagation through biomolecular complexes
Abstract
In this paper, we employ single-molecule force spectroscopy with an atomic force microscope (AFM) and steered molecular dynamics (SMD) simulations to reveal force propagation pathways through a mechanically ultrastable multidomain cellulosome protein complex. We demonstrate a new combination of network-based correlation analysis supported by AFM directional pulling experiments, which allowed us to visualize stiff paths through the protein complex along which force is transmitted. Finally, the results implicate specific force-propagation routes nonparallel to the pulling axis that are advantageous for achieving high dissociation forces.
- Authors:
-
- Ludwig-Maximilians-Univ., Munich (Germany)
- Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States)
- Ludwig-Maximilians-Univ., Munich (Germany); Univ. of Munich, Munich (Germany)
- The Weizmann Institute of Science, Rehovot (Israel)
- Publication Date:
- Research Org.:
- Univ. of Illinois at Urbana-Champaign, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1345019
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nano Letters
- Additional Journal Information:
- Journal Volume: 15; Journal Issue: 11; Journal ID: ISSN 1530-6984
- Publisher:
- American Chemical Society
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; cohesin−dockerin; force propagation; network analysis; single molecule force spectroscopy; steered molecular dynamics
Citation Formats
Schoeler, Constantin, Bernardi, Rafael C., Malinowska, Klara H., Durner, Ellis, Ott, Wolfgang, Bayer, Edward A., Schulten, Klaus, Nash, Michael A., and Gaub, Hermann E. Mapping mechanical force propagation through biomolecular complexes. United States: N. p., 2015.
Web. doi:10.1021/acs.nanolett.5b02727.
Schoeler, Constantin, Bernardi, Rafael C., Malinowska, Klara H., Durner, Ellis, Ott, Wolfgang, Bayer, Edward A., Schulten, Klaus, Nash, Michael A., & Gaub, Hermann E. Mapping mechanical force propagation through biomolecular complexes. United States. https://doi.org/10.1021/acs.nanolett.5b02727
Schoeler, Constantin, Bernardi, Rafael C., Malinowska, Klara H., Durner, Ellis, Ott, Wolfgang, Bayer, Edward A., Schulten, Klaus, Nash, Michael A., and Gaub, Hermann E. Tue .
"Mapping mechanical force propagation through biomolecular complexes". United States. https://doi.org/10.1021/acs.nanolett.5b02727. https://www.osti.gov/servlets/purl/1345019.
@article{osti_1345019,
title = {Mapping mechanical force propagation through biomolecular complexes},
author = {Schoeler, Constantin and Bernardi, Rafael C. and Malinowska, Klara H. and Durner, Ellis and Ott, Wolfgang and Bayer, Edward A. and Schulten, Klaus and Nash, Michael A. and Gaub, Hermann E.},
abstractNote = {In this paper, we employ single-molecule force spectroscopy with an atomic force microscope (AFM) and steered molecular dynamics (SMD) simulations to reveal force propagation pathways through a mechanically ultrastable multidomain cellulosome protein complex. We demonstrate a new combination of network-based correlation analysis supported by AFM directional pulling experiments, which allowed us to visualize stiff paths through the protein complex along which force is transmitted. Finally, the results implicate specific force-propagation routes nonparallel to the pulling axis that are advantageous for achieving high dissociation forces.},
doi = {10.1021/acs.nanolett.5b02727},
journal = {Nano Letters},
number = 11,
volume = 15,
place = {United States},
year = {Tue Aug 11 00:00:00 EDT 2015},
month = {Tue Aug 11 00:00:00 EDT 2015}
}
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