Cooperation between two periplasmic copper chaperones is required for full activity of the cbb3-type cytochrome c oxidase and copper homeostasis in Rhodobacter capsulatus
Abstract
Copper (Cu) is an essential micronutrient that functions as a cofactor in several important enzymes, like respiratory heme-copper oxygen reductases. Yet, Cu is also toxic and therefore cells engage a highly coordinated Cu uptake and delivery system to prevent the accumulation of toxic Cu concentrations. In the current work we analyzed Cu delivery to the cbb3-type cytochrome c oxidase (cbb3-Cox) of Rhodobacter capsulatus. We identified the PCuAC-like periplasmic chaperone PccA and analyzed its contribution to cbb3-Cox assembly. Our data demonstrate that PccA is a Cu-binding protein with a preference for Cu(I), which is required for efficient cbb3-Cox assembly, in particular at low Cu concentrations. By using in vivo and in vitro crosslinking we show that PccA forms a complex with the Sco1-homologue SenC. This complex is stabilized in the absence of the cbb3-Cox specific assembly factors CcoGHIS. In cells lacking SenC, the cytoplasmic Cu content is significantly increased, but the simultaneous absence of PccA prevents this Cu accumulation. Lastly, these data demonstrate that the interplay between PccA and SenC is not only required for Cu delivery during cbb3-Cox assembly, but that it also regulates Cu homeostasis in R. capsulatus.
- Authors:
-
- Institut fur Biochemie und Molekularbiologie, Freiburg (Germany); Albert-Ludwigs-Univ. Freiburg, Freiburg (Germany)
- Institut fur Biochemie und Molekularbiologie, Freiburg (Germany)
- Univ. of Pennsylvania, Philadelphia, PA (United States)
- Publication Date:
- Research Org.:
- Univ. of Pennsylvania, Philadelphia, PA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1344916
- Grant/Contract Number:
- FG02-91ER20052
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Molecular Microbiology
- Additional Journal Information:
- Journal Volume: 100; Journal Issue: 2; Journal ID: ISSN 0950-382X
- Publisher:
- Wiley
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; cbb3-type cytochrome c oxidase biogenesis; periplasmic copper chaperones; copper homeostasis; respiration; Rhodobacter capsulatus
Citation Formats
Trasnea, Petru -Iulian, Utz, Marcel, Khalfaoui-Hassani, Bahia, Lagies, Simon, Daldal, Fevzi, and Koch, Hans -Georg. Cooperation between two periplasmic copper chaperones is required for full activity of the cbb3-type cytochrome c oxidase and copper homeostasis in Rhodobacter capsulatus. United States: N. p., 2016.
Web. doi:10.1111/mmi.13321.
Trasnea, Petru -Iulian, Utz, Marcel, Khalfaoui-Hassani, Bahia, Lagies, Simon, Daldal, Fevzi, & Koch, Hans -Georg. Cooperation between two periplasmic copper chaperones is required for full activity of the cbb3-type cytochrome c oxidase and copper homeostasis in Rhodobacter capsulatus. United States. https://doi.org/10.1111/mmi.13321
Trasnea, Petru -Iulian, Utz, Marcel, Khalfaoui-Hassani, Bahia, Lagies, Simon, Daldal, Fevzi, and Koch, Hans -Georg. Sun .
"Cooperation between two periplasmic copper chaperones is required for full activity of the cbb3-type cytochrome c oxidase and copper homeostasis in Rhodobacter capsulatus". United States. https://doi.org/10.1111/mmi.13321. https://www.osti.gov/servlets/purl/1344916.
@article{osti_1344916,
title = {Cooperation between two periplasmic copper chaperones is required for full activity of the cbb3-type cytochrome c oxidase and copper homeostasis in Rhodobacter capsulatus},
author = {Trasnea, Petru -Iulian and Utz, Marcel and Khalfaoui-Hassani, Bahia and Lagies, Simon and Daldal, Fevzi and Koch, Hans -Georg},
abstractNote = {Copper (Cu) is an essential micronutrient that functions as a cofactor in several important enzymes, like respiratory heme-copper oxygen reductases. Yet, Cu is also toxic and therefore cells engage a highly coordinated Cu uptake and delivery system to prevent the accumulation of toxic Cu concentrations. In the current work we analyzed Cu delivery to the cbb3-type cytochrome c oxidase (cbb3-Cox) of Rhodobacter capsulatus. We identified the PCuAC-like periplasmic chaperone PccA and analyzed its contribution to cbb3-Cox assembly. Our data demonstrate that PccA is a Cu-binding protein with a preference for Cu(I), which is required for efficient cbb3-Cox assembly, in particular at low Cu concentrations. By using in vivo and in vitro crosslinking we show that PccA forms a complex with the Sco1-homologue SenC. This complex is stabilized in the absence of the cbb3-Cox specific assembly factors CcoGHIS. In cells lacking SenC, the cytoplasmic Cu content is significantly increased, but the simultaneous absence of PccA prevents this Cu accumulation. Lastly, these data demonstrate that the interplay between PccA and SenC is not only required for Cu delivery during cbb3-Cox assembly, but that it also regulates Cu homeostasis in R. capsulatus.},
doi = {10.1111/mmi.13321},
journal = {Molecular Microbiology},
number = 2,
volume = 100,
place = {United States},
year = {Sun Feb 28 00:00:00 EST 2016},
month = {Sun Feb 28 00:00:00 EST 2016}
}
Web of Science
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