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Title: Use of carbon monoxide and hydrogen by a bacteria-animal symbiosis from seagrass sediments

The gutless marine worm Olavius algarvensis lives in symbiosis with chemosynthetic bacteria that provide nutrition by fixing carbon dioxide (CO 2) into biomass using reduced sulfur compounds as energy sources. A recent metaproteomic analysis of the O. algarvensis symbiosis indicated that carbon monoxide (CO) and hydrogen (H 2) might also be used as energy sources. We provide direct evidence that the O. algarvensis symbiosis consumes CO and H 2. Single cell imaging using nanoscale secondary ion mass spectrometry revealed that one of the symbionts, the γ3-symbiont, uses the energy from CO oxidation to fix CO 2. Pore water analysis revealed considerable in-situ concentrations of CO and H 2 in the O. algarvensis environment, Mediterranean seagrass sediments. Pore water H 2 concentrations (89-2147 nM) were up to two orders of magnitude higher than in seawater, and up to 36-fold higher than previously known from shallow-water marine sediments. Pore water CO concentrations (17-51 nM) were twice as high as in the overlying seawater (no literature data from other shallow-water sediments are available for comparison). Ex-situ incubation experiments showed that dead seagrass rhizomes produced large amounts of CO. Lastly, CO production from decaying plant material could thus be a significant energy source formore » microbial primary production in seagrass sediments.« less
Authors:
 [1] ;  [2] ;  [3] ;  [3] ;  [3] ;  [4] ;  [3] ;  [3] ;  [3]
  1. Max Planck Institute for Marine Microbiology, Bremen (Germany); Univ. of Calgary, Calgary, AB (Canada)
  2. Max Planck Institute for Marine Microbiology, Bremen (Germany); Univ. of Vienna, Vienna (Austria)
  3. Max Planck Institute for Marine Microbiology, Bremen (Germany)
  4. Max Planck Institute for Marine Microbiology, Bremen (Germany); HYDRA Institute for Marine Sciences, Campo nell'Elba (Italy)
Publication Date:
Grant/Contract Number:
AC02-05CH11231; GBMF3811
Type:
Accepted Manuscript
Journal Name:
Environmental Microbiology
Additional Journal Information:
Journal Volume: 17; Journal Issue: 12; Journal ID: ISSN 1462-2912
Publisher:
Wiley
Research Org:
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC); Gordon and Betty Moore Foundation; Studienstiftung des deutschen Volkes
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES
OSTI Identifier:
1344909