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Title: Contrasting allelic distribution of CO/ Hd1 homologues in Miscanthus sinensis from the East Asian mainland and the Japanese archipelago

The genus Miscanthus is a perennial C 4 grass native to eastern Asia and is a promising candidate bioenergy crop for cool temperate areas. Flowering time is a crucial factor governing regional and seasonal adaptation; in addition, it is also a key target trait for extending the vegetative phase to improve biomass potential. Homologues of CONSTANS (CO)/Heading date 1(Hd1) were cloned from Miscanthus sinensis and named MsiHd1. Sequences of MsiHd1 homologues were compared among 24 wild M. sinensis accessions from Japan, 14 from China, and three from South Korea. Two to five MsiHd1 alleles in each accession were identified, suggesting that MsiHd1 consists of at least three loci in the Miscanthus genome. Verifying the open reading frame in MsiHd1, they were classified as putative functional alleles without mutations or non-functional alleles caused by indels. The Neighbor-Joining tree indicated that one of the multiple MsiHd1 loci is a pseudogene locus without any functional alleles. The pseudogene locus was named MsiHd1b, and the other loci were considered to be part of the MsiHd1a multi-locus family. Interestingly, in most Japanese accessions 50% or more of the MsiHd1a alleles were non-functional, whereas accessions from the East Asian mainland harboured only functional alleles. Five novelmore » miniature inverted transposable elements (MITEs) ( MsiMITE1-MsiMITE5) were observed in MsiHd1a/b. MsiMITE1, detected in exon 1 of MsiHd1a, was only observed in Japanese accessions and its revertant alleles derived from retransposition were predominantly in Chinese accessions. In conclusion, these differences in MsiHd1a show that the dependency on functional MsiHd1a alleles is different between accessions from the East Asian mainland and Japan.« less
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [5] ;  [5] ;  [5] ;  [1] ;  [1] ;  [6] ;  [1]
  1. Hokkaido Univ., Hokkaido (Japan)
  2. Univ. of Illinois, Urbana-Champaign, IL (United States). Dept. of Crop Sciences
  3. Huazhong Agricultural Univ., Hubei (China)
  4. China Seed Group Co. Ltd., Hubei (China)
  5. Kangwon National Univ., Gangwon (South Korea)
  6. Univ. of Illinois, Urbana-Champaign, Urbana, IL (United States)
Publication Date:
Grant/Contract Number:
SC0012379
Type:
Accepted Manuscript
Journal Name:
Journal of Experimental Botany
Additional Journal Information:
Journal Volume: 66; Journal Issue: 14; Journal ID: ISSN 0022-0957
Publisher:
Oxford University Press
Research Org:
Univ. of Illinois, Urbana-Champaign, IL (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; bioenergy; MITEs.; Miscanthus sinensis; flowering time gene; gene duplication; geographical allelic differentiation
OSTI Identifier:
1344522