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Title: Genetic structure of Miscanthus sinensis and Miscanthus sacchariflorus in Japan indicates a gradient of bidirectional but asymmetric introgression

Abstract

Unilateral introgression from diploids to tetraploids has been hypothesized to be an important evolutionary mechanism in plants. However, few examples have been definitively identified, perhaps because data of sufficient depth and breadth were difficult to obtain before the advent of affordable high-density genotyping. Throughout Japan, tetraploid Miscanthus sacchariflorus and diploid Miscanthus sinensis are common, and occasionally hybridize. In this study, 667 M. sinensis and 78 M. sacchariflorus genotypes from Japan were characterized using 20 704 SNPs and ten plastid microsatellites. Similarity of SNP genotypes between diploid and tetraploid M. sacchariflorus indicated that the tetraploids originated through autopolyploidy. Structure analysis indicated a gradient of introgression from diploid M. sinensis into tetraploid M. sacchariflorus throughout Japan; most tetraploids had some M. sinensis DNA. Among phenotypically M. sacchariflorus tetraploids, M. sinensis ancestry averaged 7% and ranged from 1-39%, with introgression greatest in southern Japan. Unexpectedly, rare (~1%) diploid M. sinensis individuals from northern Japan were found with 6-27% M. sacchariflorus ancestry. Population structure of M. sinensis in Japan included three groups, and was driven primarily by distance, and secondarily by geographic barriers such as mountains and straits. Miscanthus speciation is a complex and dynamic process. In contrast to limited introgression between diploid M.more » sacchariflorus and M. sinensis in northern China, selection for adaptation to a moderate maritime climate probably favoured cross-ploidy introgressants in southern Japan. Ultimately, these results will help guide the selection of Miscanthus accessions for the breeding of biomass cultivars.« less

Authors:
 [1];  [2];  [3];  [4];  [5];  [5];  [6];  [7];  [8];  [8];  [8];  [9];  [1]
  1. Univ. of Illinois, Urbana-Champaign, Urbana, IL (United States)
  2. Brigham Young Univ., Provo, UT (United States)
  3. Univ. of Miyazaki, Miyazaki (Japan)
  4. Ehime Univ., Ehime (Japan)
  5. Aarhus Univ. (Denmark)
  6. Huazhong Agricultural Univ., Hubei (China)
  7. China Seed Group Co. Ltd., Hubei (China)
  8. Kangwon National Univ., Gangwon (South Korea)
  9. Hokkaido Univ., Hokkaido (Japan)
Publication Date:
Research Org.:
Univ. of Illinois, Urbana-Champaign, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1344512
Grant/Contract Number:  
SC0012379
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Experimental Botany
Additional Journal Information:
Journal Volume: 66; Journal Issue: 14; Journal ID: ISSN 0022-0957
Publisher:
Oxford University Press
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; biomass crop; poaceae; RAD-seq.; cross-ploidy introgression; hybridization; polyploidy; population genetics

Citation Formats

Clark, Lindsay V., Stewart, J. Ryan, Nishiwaki, Aya, Toma, Yo, Kjeldsen, Jens Bonderup, Jorgensen, Uffe, Zhao, Hua, Peng, Junhua, Yoo, Ji Hye, Heo, Kweon, Yu, Chang Yeon, Yamada, Toshihiko, and Sacks, Erik J. Genetic structure of Miscanthus sinensis and Miscanthus sacchariflorus in Japan indicates a gradient of bidirectional but asymmetric introgression. United States: N. p., 2015. Web. doi:10.1093/jxb/eru511.
Clark, Lindsay V., Stewart, J. Ryan, Nishiwaki, Aya, Toma, Yo, Kjeldsen, Jens Bonderup, Jorgensen, Uffe, Zhao, Hua, Peng, Junhua, Yoo, Ji Hye, Heo, Kweon, Yu, Chang Yeon, Yamada, Toshihiko, & Sacks, Erik J. Genetic structure of Miscanthus sinensis and Miscanthus sacchariflorus in Japan indicates a gradient of bidirectional but asymmetric introgression. United States. doi:10.1093/jxb/eru511.
Clark, Lindsay V., Stewart, J. Ryan, Nishiwaki, Aya, Toma, Yo, Kjeldsen, Jens Bonderup, Jorgensen, Uffe, Zhao, Hua, Peng, Junhua, Yoo, Ji Hye, Heo, Kweon, Yu, Chang Yeon, Yamada, Toshihiko, and Sacks, Erik J. Sat . "Genetic structure of Miscanthus sinensis and Miscanthus sacchariflorus in Japan indicates a gradient of bidirectional but asymmetric introgression". United States. doi:10.1093/jxb/eru511. https://www.osti.gov/servlets/purl/1344512.
@article{osti_1344512,
title = {Genetic structure of Miscanthus sinensis and Miscanthus sacchariflorus in Japan indicates a gradient of bidirectional but asymmetric introgression},
author = {Clark, Lindsay V. and Stewart, J. Ryan and Nishiwaki, Aya and Toma, Yo and Kjeldsen, Jens Bonderup and Jorgensen, Uffe and Zhao, Hua and Peng, Junhua and Yoo, Ji Hye and Heo, Kweon and Yu, Chang Yeon and Yamada, Toshihiko and Sacks, Erik J.},
abstractNote = {Unilateral introgression from diploids to tetraploids has been hypothesized to be an important evolutionary mechanism in plants. However, few examples have been definitively identified, perhaps because data of sufficient depth and breadth were difficult to obtain before the advent of affordable high-density genotyping. Throughout Japan, tetraploid Miscanthus sacchariflorus and diploid Miscanthus sinensis are common, and occasionally hybridize. In this study, 667 M. sinensis and 78 M. sacchariflorus genotypes from Japan were characterized using 20 704 SNPs and ten plastid microsatellites. Similarity of SNP genotypes between diploid and tetraploid M. sacchariflorus indicated that the tetraploids originated through autopolyploidy. Structure analysis indicated a gradient of introgression from diploid M. sinensis into tetraploid M. sacchariflorus throughout Japan; most tetraploids had some M. sinensis DNA. Among phenotypically M. sacchariflorus tetraploids, M. sinensis ancestry averaged 7% and ranged from 1-39%, with introgression greatest in southern Japan. Unexpectedly, rare (~1%) diploid M. sinensis individuals from northern Japan were found with 6-27% M. sacchariflorus ancestry. Population structure of M. sinensis in Japan included three groups, and was driven primarily by distance, and secondarily by geographic barriers such as mountains and straits. Miscanthus speciation is a complex and dynamic process. In contrast to limited introgression between diploid M. sacchariflorus and M. sinensis in northern China, selection for adaptation to a moderate maritime climate probably favoured cross-ploidy introgressants in southern Japan. Ultimately, these results will help guide the selection of Miscanthus accessions for the breeding of biomass cultivars.},
doi = {10.1093/jxb/eru511},
journal = {Journal of Experimental Botany},
number = 14,
volume = 66,
place = {United States},
year = {2015},
month = {1}
}

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