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Title: Physicochemical structural changes of cellulosic substrates during enzymatic saccharification

Enzymatic hydrolysis represents one of the major steps and barriers in the commercialization process of converting cellulosic substrates into biofuels and other value added products. It is usually achieved by a synergistic action of enzyme mixture typically consisting of multiple enzymes such as glucanase, cellobiohydrolase and β-glucosidase with different mode of actions. Due to the innate biomass recalcitrance, enzymatic hydrolysis normally starts with an initial fast rate of hydrolysis followed by a rapid decrease of rate toward the end of hydrolysis. With majority of literature studies focusing on the effect of key substrate characteristics on the initial rate or final yield of enzymatic hydrolysis, information about physicochemical structural changes of cellulosic substrates during enzymatic hydrolysis is still quite limited. Consequently, what slows down the reaction rate toward the end of hydrolysis is not well understood. Lastly, this review highlights recent advances in understanding the structural changes of cellulosic substrates during the hydrolysis process, to better understand the fundamental mechanisms of enzymatic hydrolysis.
Authors:
 [1] ;  [2] ;  [2] ;  [3]
  1. Univ. of Tennessee, Knoxville, TN (United States). Department of Chemical and Biomolecular Engineering
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); UT-ORNL Joint Institute for Biological Science (United States)
  3. Univ. of Tennessee, Knoxville, TN (United States). Department of Chemical and Biomolecular Engineering; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); UT-ORNL Joint Institute for Biological Science (United States); University of Tennessee Institute of Agriculture, Knoxville, TN (United States). Department of Forestry
Publication Date:
Grant/Contract Number:
AC05-00OR22725
Type:
Accepted Manuscript
Journal Name:
Journal of Applied Biotechnology & Bioengineering
Additional Journal Information:
Journal Volume: 1; Journal Issue: 3; Journal ID: ISSN 2455-7005
Publisher:
MedCrave
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; Glucanase; Cellobiohydrolase; β-glucosidase; Lingocellulosics; Fucose
OSTI Identifier:
1343547