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Title: The genome sequence of Saccharomyces eubayanus and the domestication of lager-brewing yeasts

The dramatic phenotypic changes that occur in organisms during domestication leave indelible imprints on their genomes. Although many domesticated plants and animals have been systematically compared with their wild genetic stocks, the molecular and genomic processes underlying fungal domestication have received less attention. Here, we present a nearly complete genome assembly for the recently described yeast species Saccharomyces eubayanus and compare it to the genomes of multiple domesticated alloploid hybrids of S. eubayanus × S. cerevisiae ( S. pastorianus syn. S. carlsbergensis), which are used to brew lager-style beers. We find that the S. eubayanus subgenomes of lager-brewing yeasts have experienced increased rates of evolution since hybridization, and that certain genes involved in metabolism may have been particularly affected. Interestingly, the S. eubayanus subgenome underwent an especially strong shift in selection regimes, consistent with more extensive domestication of the S. cerevisiae parent prior to hybridization. In contrast to recent proposals that lager-brewing yeasts were domesticated following a single hybridization event, the radically different neutral site divergences between the subgenomes of the two major lager yeast lineages strongly favor at least two independent origins for the S. cerevisiae × S. eubayanus hybrids that brew lager beers. In conclusion, our findings demonstratemore » how this industrially important hybrid has been domesticated along similar evolutionary trajectories on multiple occasions.« less
Authors:
 [1] ;  [1] ;  [2] ;  [1] ;  [1] ;  [3] ;  [3] ;  [4] ;  [5]
  1. Univ. of Wisconsin, Madison, WI (United States)
  2. Univ. of Wisconsin, Madison, WI (United States); Univ. Nacional del Comahue-CONICET, Bariloche (Argentina)
  3. Univ. of Wisconsin, Madison, WI (United States). Biotechnology Center
  4. Univ. Nacional del Comahue-CONICET, Bariloche (Argentina)
  5. Univ. of Wisconsin, Madison, WI (United States). DOE Great Lakes Bioenergy Research Center
Publication Date:
Grant/Contract Number:
FC02-07ER64494; DEB-1253634
Type:
Accepted Manuscript
Journal Name:
Molecular Biology and Evolution
Additional Journal Information:
Journal Volume: 32; Journal Issue: 11; Journal ID: ISSN 0737-4038
Publisher:
Oxford University Press
Research Org:
Univ. of Wisconsin, Madison, WI (United States). DOE Great Lakes Bioenergy Research Center
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); National Science Foundation (NSF)
Contributing Orgs:
Univ. Nacional del Comahue-CONICET, Bariloche (Argentina)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Saccharomyces eubayanus; domestication; genome assembly; hybridization; lager brewing
OSTI Identifier:
1343408
Alternate Identifier(s):
OSTI ID: 1438256