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Title: Description of the first fungal dye-decolorizing peroxidase oxidizing manganese(II)

Abstract

Two phylogenetically divergent genes of the new family of dye-decolorizing peroxidases (DyPs) were found during comparison of the four DyP genes identified in the Pleurotus ostreatus genome with over 200 DyP genes from other basidiomycete genomes. The heterologously expressed enzymes ( Pleos-DyP1 and Pleos-DyP4, following the genome nomenclature) efficiently oxidize anthraquinoid dyes (such as Reactive Blue 19), which are characteristic DyP substrates, as well as low redox-potential dyes (such as 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)) and substituted phenols. However, only Pleos-DyP4 oxidizes the high redox-potential dye Reactive Black 5, at the same time that it displays high thermal and pH stability. Unexpectedly, both enzymes also oxidize Mn 2+ to Mn 3+, albeit with very different catalytic efficiencies. Pleos-DyP4 presents a Mn 2+ turnover (56 s –1) nearly in the same order of the two other Mn 2+-oxidizing peroxidase families identified in the P. ostreatus genome: manganese peroxidases (100 s–1 average turnover) and versatile peroxidases (145 s –1 average turnover), whose genes were also heterologously expressed. Oxidation of Mn 2+ has been reported for an Amycolatopsis DyP (24 s –1) and claimed for other bacterial DyPs, albeit with lower activities, but this is the first time that Mn 2+ oxidation is reported for amore » fungal DyP. Interestingly, Pleos-DyP4 (together with ligninolytic peroxidases) is detected in the secretome of P. ostreatus grown on different lignocellulosic substrates. In conclusion, it is suggested that generation of Mn 3+ oxidizers plays a role in the P. ostreatus white-rot lifestyle since three different families of Mn 2+-oxidizing peroxidase genes are present in its genome being expressed during lignocellulose degradation.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [1]
  1. Centro de Investigaciones Biologicas, Madrid (Spain)
Publication Date:
Research Org.:
Center for Biological Research (CIB), Madrid (Spain)
Sponsoring Org.:
USDOE Office of Science (SC); Spanish Ministry of Economy and Competitiveness (MINECO) (Spain)
OSTI Identifier:
1343259
Grant/Contract Number:  
AC02-05CH11231; BIO2011-26694
Resource Type:
Accepted Manuscript
Journal Name:
Applied Microbiology and Biotechnology
Additional Journal Information:
Journal Volume: 99; Journal Issue: 21; Journal ID: ISSN 0175-7598
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; Pleurotus ostreatus; Dye-decolorizing peroxidases; Genome mining; Lignocellulose secretome; Manganese(II) oxidation; Evolutionary history

Citation Formats

Fernandez-Fueyo, Elena, Linde, Dolores, Almendral, David, Lopez-Lucendo, María F., Ruiz-Duenas, Francisco J., and Martinez, Angel T. Description of the first fungal dye-decolorizing peroxidase oxidizing manganese(II). United States: N. p., 2015. Web. doi:10.1007/s00253-015-6665-3.
Fernandez-Fueyo, Elena, Linde, Dolores, Almendral, David, Lopez-Lucendo, María F., Ruiz-Duenas, Francisco J., & Martinez, Angel T. Description of the first fungal dye-decolorizing peroxidase oxidizing manganese(II). United States. doi:10.1007/s00253-015-6665-3.
Fernandez-Fueyo, Elena, Linde, Dolores, Almendral, David, Lopez-Lucendo, María F., Ruiz-Duenas, Francisco J., and Martinez, Angel T. Wed . "Description of the first fungal dye-decolorizing peroxidase oxidizing manganese(II)". United States. doi:10.1007/s00253-015-6665-3. https://www.osti.gov/servlets/purl/1343259.
@article{osti_1343259,
title = {Description of the first fungal dye-decolorizing peroxidase oxidizing manganese(II)},
author = {Fernandez-Fueyo, Elena and Linde, Dolores and Almendral, David and Lopez-Lucendo, María F. and Ruiz-Duenas, Francisco J. and Martinez, Angel T.},
abstractNote = {Two phylogenetically divergent genes of the new family of dye-decolorizing peroxidases (DyPs) were found during comparison of the four DyP genes identified in the Pleurotus ostreatus genome with over 200 DyP genes from other basidiomycete genomes. The heterologously expressed enzymes (Pleos-DyP1 and Pleos-DyP4, following the genome nomenclature) efficiently oxidize anthraquinoid dyes (such as Reactive Blue 19), which are characteristic DyP substrates, as well as low redox-potential dyes (such as 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)) and substituted phenols. However, only Pleos-DyP4 oxidizes the high redox-potential dye Reactive Black 5, at the same time that it displays high thermal and pH stability. Unexpectedly, both enzymes also oxidize Mn2+ to Mn3+, albeit with very different catalytic efficiencies. Pleos-DyP4 presents a Mn2+ turnover (56 s–1) nearly in the same order of the two other Mn2+-oxidizing peroxidase families identified in the P. ostreatus genome: manganese peroxidases (100 s–1 average turnover) and versatile peroxidases (145 s–1 average turnover), whose genes were also heterologously expressed. Oxidation of Mn2+ has been reported for an Amycolatopsis DyP (24 s–1) and claimed for other bacterial DyPs, albeit with lower activities, but this is the first time that Mn2+ oxidation is reported for a fungal DyP. Interestingly, Pleos-DyP4 (together with ligninolytic peroxidases) is detected in the secretome of P. ostreatus grown on different lignocellulosic substrates. In conclusion, it is suggested that generation of Mn3+ oxidizers plays a role in the P. ostreatus white-rot lifestyle since three different families of Mn2+-oxidizing peroxidase genes are present in its genome being expressed during lignocellulose degradation.},
doi = {10.1007/s00253-015-6665-3},
journal = {Applied Microbiology and Biotechnology},
number = 21,
volume = 99,
place = {United States},
year = {2015},
month = {5}
}

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