Cell-wall properties contributing to improved deconstruction by alkaline pre-treatment and enzymatic hydrolysis in diverse maize ( Zea mays L.) lines

doi:10.1093/jxb/erv016

Title: Cell-wall properties contributing to improved deconstruction by alkaline pre-treatment and enzymatic hydrolysis in diverse maize ( Zea mays L.) lines

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Abstract

A maize (Zea mays L. subsp. mays) diversity panel consisting of 26 maize lines exhibiting a wide range of cell-wall properties and responses to hydrolysis by cellulolytic enzymes was employed to investigate the relationship between cell-wall properties, cell-wall responses to mild NaOH pre-treatment, and enzymatic hydrolysis yields. Enzymatic hydrolysis of the cellulose in the untreated maize was found to be positively correlated with the water retention value, which is a measure of cell-wall susceptibility to swelling. It was also positively correlated with the lignin syringyl/guaiacyl ratio and negatively correlated with the initial cell-wall lignin, xylan, acetate, and p-coumaric acid (pCA) content, as well as pCA released from the cell wall by pre-treatment. The hydrolysis yield following pre-treatment exhibited statistically significant negative correlations to the lignin content after pre-treatment and positive correlations to the solubilized ferulic acid and pCA. Several unanticipated results were observed, including a positive correlation between initial lignin and acetate content, lack of correlation between acetate content and initial xylan content, and negative correlation between each of these three variables to the hydrolysis yields for untreated maize. Also, another surprising result was that pCA release was negatively correlated with hydrolysis yields for untreated maize and, along with ferulicmore »« less

Authors:

Li, Muyang ^[1]; Heckwolf, Marlies ^[2]; Crowe, Jacob D. ^[3]; Williams, Daniel L. ^[4]; Magee, Timothy D. ^[3]; Kaeppler, Shawn M. ^[5]; de Leon, Natalia ^[5]; Hodge, David B. ^[6]

Michigan State Univ., East Lansing, MI (United States). Dept. of Biosystems & Agricultural Engineering; DOE-Great Lakes Bioenergy Research Center, Madison, WI (United States)
DOE-Great Lakes Bioenergy Research Center, Madison, WI (United States)
Michigan State Univ., East Lansing, MI (United States). Dept. of Chemical Engineering & Materials Science
DOE-Great Lakes Bioenergy Research Center, Madison, WI (United States); Michigan State Univ., East Lansing, MI (United States). Dept. of Chemical Engineering & Materials Science
DOE-Great Lakes Bioenergy Research Center, Madison, WI (United States); Univ. of Wisconsin, Madison, WI (United States). Dept. of Agronomy
Michigan State Univ., East Lansing, MI (United States). Dept. of Biosystems & Agricultural Engineering; DOE-Great Lakes Bioenergy Research Center, Madison, WI (United States); Michigan State Univ., East Lansing, MI (United States). Dept. of Chemical Engineering & Materials Science; Lulea Univ. of Technology, Lulea (Sweden). Division of Sustainable Process Engineering

Publication Date:: 2015-02-20

Research Org.:: Michigan State Univ., East Lansing, MI (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Science Foundation (NSF)

OSTI Identifier:: 1343091

Grant/Contract Number:: FC02-07ER64494; NSF CBET 1336622

Resource Type:: Accepted Manuscript

Journal Name:: Journal of Experimental Botany

Additional Journal Information:: Journal Volume: 66; Journal Issue: 14; Journal ID: ISSN 0022-0957

Publisher:: Oxford University Press

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 09 BIOMASS FUELS; Biofuels; cell-wall recalcitrance; enzymatic hydrolysis; maize; plant cell-wall characterization; pre-treatment

Citation Formats


                    Li, Muyang, Heckwolf, Marlies, Crowe, Jacob D., Williams, Daniel L., Magee, Timothy D., Kaeppler, Shawn M., de Leon, Natalia, and Hodge, David B. Cell-wall properties contributing to improved deconstruction by alkaline pre-treatment and enzymatic hydrolysis in diverse maize ( Zea mays L.) lines.  United States: N. p., 2015. 
        Web.  doi:10.1093/jxb/erv016.

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                    Li, Muyang, Heckwolf, Marlies, Crowe, Jacob D., Williams, Daniel L., Magee, Timothy D., Kaeppler, Shawn M., de Leon, Natalia, & Hodge, David B. Cell-wall properties contributing to improved deconstruction by alkaline pre-treatment and enzymatic hydrolysis in diverse maize ( Zea mays L.) lines.  United States.  doi:10.1093/jxb/erv016.

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                    Li, Muyang, Heckwolf, Marlies, Crowe, Jacob D., Williams, Daniel L., Magee, Timothy D., Kaeppler, Shawn M., de Leon, Natalia, and Hodge, David B. Fri .  
        "Cell-wall properties contributing to improved deconstruction by alkaline pre-treatment and enzymatic hydrolysis in diverse maize ( Zea mays L.) lines".  United States.  doi:10.1093/jxb/erv016.  https://www.osti.gov/servlets/purl/1343091.

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@article{osti_1343091,

  title        = {Cell-wall properties contributing to improved deconstruction by alkaline pre-treatment and enzymatic hydrolysis in diverse maize ( Zea mays L.) lines},

  author       = {Li, Muyang and Heckwolf, Marlies and Crowe, Jacob D. and Williams, Daniel L. and Magee, Timothy D. and Kaeppler, Shawn M. and de Leon, Natalia and Hodge, David B.},

  abstractNote = {A maize (Zea mays L. subsp. mays) diversity panel consisting of 26 maize lines exhibiting a wide range of cell-wall properties and responses to hydrolysis by cellulolytic enzymes was employed to investigate the relationship between cell-wall properties, cell-wall responses to mild NaOH pre-treatment, and enzymatic hydrolysis yields. Enzymatic hydrolysis of the cellulose in the untreated maize was found to be positively correlated with the water retention value, which is a measure of cell-wall susceptibility to swelling. It was also positively correlated with the lignin syringyl/guaiacyl ratio and negatively correlated with the initial cell-wall lignin, xylan, acetate, and p-coumaric acid (pCA) content, as well as pCA released from the cell wall by pre-treatment. The hydrolysis yield following pre-treatment exhibited statistically significant negative correlations to the lignin content after pre-treatment and positive correlations to the solubilized ferulic acid and pCA. Several unanticipated results were observed, including a positive correlation between initial lignin and acetate content, lack of correlation between acetate content and initial xylan content, and negative correlation between each of these three variables to the hydrolysis yields for untreated maize. Also, another surprising result was that pCA release was negatively correlated with hydrolysis yields for untreated maize and, along with ferulic acid release, was positively correlated with the pre-treated maize hydrolysis yields. In conclusion, this indicates that these properties that may negatively contribute to the recalcitrance in untreated cell walls may positively contribute to their deconstruction by alkaline pre-treatment},

  doi          = {10.1093/jxb/erv016},

  journal      = {Journal of Experimental Botany},

  number       = 14,

  volume       = 66,

  place        = {United States},

  year         = {2015},

  month        = {2}

}

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DOI: 10.1093/jxb/erv016

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