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Title: A Comparison of Single-Molecule Emission in Aluminum and Gold Zero-Mode Waveguides

We characterized the effect of gold and aluminum zero-mode waveguides (ZMWs) on the brightness of immobilized single emitters by probing fluorophores that absorb in the green and red regions of the visible spectrum. Aluminum ZMWs enhance the emission of Atto565 fluorophores upon green excitation, but they do not enhance the emission of Atto647N fluorophores upon red excitation. Gold ZMWs increase emission of both fluorophores with Atto647N showing enhancement that is threefold higher than that observed for Atto565. Our work indicates that 200 nm gold ZMWs are better suited for single-molecule fluorescence studies in the red region of the visible spectrum, while aluminum appears more suited for the green region of the visible spectrum.
Authors:
 [1] ;  [2] ;  [2] ;  [3] ;  [1]
  1. Univ. of Kentucky, Lexington, KY (United States). Dept. of Chemistry
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Nanophase Material Science
  3. Univ. of Copenhagen (Denmark). Nano-science Center and Dept. of Chemistry
Publication Date:
Grant/Contract Number:
AC05-00OR22725
Type:
Accepted Manuscript
Journal Name:
Journal of Physical Chemistry. A, Molecules, Spectroscopy, Kinetics, Environment, and General Theory
Additional Journal Information:
Journal Volume: 120; Journal Issue: 34; Journal ID: ISSN 1089-5639
Publisher:
American Chemical Society
Research Org:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)
Sponsoring Org:
USDOE Office of Science (SC)
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
OSTI Identifier:
1342673

Martin, William Elliott, Srijanto, Bernadeta R., Collier, C. Patrick, Vosch, Tom, and Richards, Christopher I.. A Comparison of Single-Molecule Emission in Aluminum and Gold Zero-Mode Waveguides. United States: N. p., Web. doi:10.1021/acs.jpca.6b03309.
Martin, William Elliott, Srijanto, Bernadeta R., Collier, C. Patrick, Vosch, Tom, & Richards, Christopher I.. A Comparison of Single-Molecule Emission in Aluminum and Gold Zero-Mode Waveguides. United States. doi:10.1021/acs.jpca.6b03309.
Martin, William Elliott, Srijanto, Bernadeta R., Collier, C. Patrick, Vosch, Tom, and Richards, Christopher I.. 2016. "A Comparison of Single-Molecule Emission in Aluminum and Gold Zero-Mode Waveguides". United States. doi:10.1021/acs.jpca.6b03309. https://www.osti.gov/servlets/purl/1342673.
@article{osti_1342673,
title = {A Comparison of Single-Molecule Emission in Aluminum and Gold Zero-Mode Waveguides},
author = {Martin, William Elliott and Srijanto, Bernadeta R. and Collier, C. Patrick and Vosch, Tom and Richards, Christopher I.},
abstractNote = {We characterized the effect of gold and aluminum zero-mode waveguides (ZMWs) on the brightness of immobilized single emitters by probing fluorophores that absorb in the green and red regions of the visible spectrum. Aluminum ZMWs enhance the emission of Atto565 fluorophores upon green excitation, but they do not enhance the emission of Atto647N fluorophores upon red excitation. Gold ZMWs increase emission of both fluorophores with Atto647N showing enhancement that is threefold higher than that observed for Atto565. Our work indicates that 200 nm gold ZMWs are better suited for single-molecule fluorescence studies in the red region of the visible spectrum, while aluminum appears more suited for the green region of the visible spectrum.},
doi = {10.1021/acs.jpca.6b03309},
journal = {Journal of Physical Chemistry. A, Molecules, Spectroscopy, Kinetics, Environment, and General Theory},
number = 34,
volume = 120,
place = {United States},
year = {2016},
month = {8}
}