Procedures for cryogenic X-ray ptychographic imaging of biological samples

Yusuf, M.; Zhang, F.; Chen, B.; Bhartiya, A.; Cunnea, K.; Wagner, U.; Cacho-Nerin, F.; Schwenke, J.; Robinson, I. K.

doi:10.1107/S2052252516020029

Title: Procedures for cryogenic X-ray ptychographic imaging of biological samples

Abstract

Biological sample-preparation procedures have been developed for imaging human chromosomes under cryogenic conditions. A new experimental setup, developed for imaging frozen samples using beamline I13 at Diamond Light Source, is described. This paper describes the equipment and experimental procedures as well as the authors' first ptychographic reconstructions using X-rays.

Authors:: Yusuf, M.; Zhang, F.; Chen, B.; Bhartiya, A.; Cunnea, K.; Wagner, U.; Cacho-Nerin, F.; Schwenke, J.; Robinson, I. K.

Publication Date:: Thu Jan 12 00:00:00 EST 2017

Research Org.:: Brookhaven National Lab. (BNL), Upton, NY (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:: 1339020

Alternate Identifier(s):: OSTI ID: 1342640

Report Number(s):: BNL-113441-2017-JA
Journal ID: ISSN 2052-2525; IUCRAJ; PII: S2052252516020029

Grant/Contract Number:: SC00112704; SC0012704; BB/ H022597/1

Resource Type:: Published Article

Journal Name:: IUCrJ

Additional Journal Information:: Journal Name: IUCrJ Journal Volume: 4 Journal Issue: 2; Journal ID: ISSN 2052-2525

Publisher:: International Union of Crystallography (IUCr)

Country of Publication:: United Kingdom

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION; human chromosomes; cell nuclei; chromosome imaging; X-ray ptychography; plunge freezing

Citation Formats


                    Yusuf, M., Zhang, F., Chen, B., Bhartiya, A., Cunnea, K., Wagner, U., Cacho-Nerin, F., Schwenke, J., and Robinson, I. K. Procedures for cryogenic X-ray ptychographic imaging of biological samples.  United Kingdom: N. p., 2017. 
Web.  doi:10.1107/S2052252516020029.

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                    Yusuf, M., Zhang, F., Chen, B., Bhartiya, A., Cunnea, K., Wagner, U., Cacho-Nerin, F., Schwenke, J., & Robinson, I. K. Procedures for cryogenic X-ray ptychographic imaging of biological samples.  United Kingdom.  https://doi.org/10.1107/S2052252516020029

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                    Yusuf, M., Zhang, F., Chen, B., Bhartiya, A., Cunnea, K., Wagner, U., Cacho-Nerin, F., Schwenke, J., and Robinson, I. K. Thu .  
"Procedures for cryogenic X-ray ptychographic imaging of biological samples".  United Kingdom.  https://doi.org/10.1107/S2052252516020029.

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@article{osti_1339020,

  title        = {Procedures for cryogenic X-ray ptychographic imaging of biological samples},

  author       = {Yusuf, M. and Zhang, F. and Chen, B. and Bhartiya, A. and Cunnea, K. and Wagner, U. and Cacho-Nerin, F. and Schwenke, J. and Robinson, I. K.},

  abstractNote = {Biological sample-preparation procedures have been developed for imaging human chromosomes under cryogenic conditions. A new experimental setup, developed for imaging frozen samples using beamline I13 at Diamond Light Source, is described. This paper describes the equipment and experimental procedures as well as the authors' first ptychographic reconstructions using X-rays.},

  doi          = {10.1107/S2052252516020029},

  journal      = {IUCrJ},

  number       = 2,

  volume       = 4,

  place        = {United Kingdom},

  year         = {Thu Jan 12 00:00:00 EST 2017},

  month        = {Thu Jan 12 00:00:00 EST 2017}

}

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Works referencing / citing this record:

Use of 3D imaging for providing insights into high-order structure of mitotic chromosomes
journal, September 2018

Yusuf, Mohammed; Kaneyoshi, Kohei; Fukui, Kiichi
Chromosoma, Vol. 128, Issue 1
DOI: 10.1007/s00412-018-0678-5

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Title: Procedures for cryogenic X-ray ptychographic imaging of biological samples

Abstract

Citation Formats

3D Analysis of chromosome architecture: advantages and limitations with SEM journal, January 2005

X-ray structure of a tetranucleosome and its implications for the chromatin fibre journal, July 2005

Three-Dimensional Visualization of a Human Chromosome Using Coherent X-Ray Diffraction journal, January 2009

The Bionanoprobe: hard X-ray fluorescence nanoprobe with cryogenic capabilities journal, December 2013

Simultaneous cryo X-ray ptychographic and fluorescence microscopy of green algae journal, February 2015

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An improved ptychographical phase retrieval algorithm for diffractive imaging journal, September 2009

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EM measurements define the dimensions of the "30-nm" chromatin fiber: Evidence for a compact, interdigitated structure journal, April 2006

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Electron microscopy of high pressure frozen samples: bridging the gap between cellular ultrastructure and atomic resolution journal, September 2008

Soft X-ray microscopy with a cryo scanning transmission X-ray microscope: I. Instrumentation, imaging and spectroscopy journal, January 2000

Tomography of a Cryo-immobilized Yeast Cell Using Ptychographic Coherent X-Ray Diffractive Imaging journal, November 2015

Chromatin history: our view from the bridge journal, October 2003

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Soft X-Ray Diffraction Microscopy of a Frozen Hydrated Yeast Cell journal, November 2009

Cryogenic x-ray diffraction microscopy utilizing high-pressure cryopreservation journal, October 2014

Solenoidal model for superstructure in chromatin. journal, June 1976

The future is cold: cryo-preparation methods for transmission electron microscopy of cells journal, September 2011

Cryo-scanning x-ray diffraction microscopy of frozen-hydrated yeast: CRYO-SCANNING X-RAY DIFFRACTION MICROSCOPY journal, December 2012

Three-dimensional cellular ultrastructure resolved by X-ray microscopy journal, November 2010

Towards native-state imaging in biological context in the electron microscope journal, November 2009

Use of 3D imaging for providing insights into high-order structure of mitotic chromosomes journal, September 2018

3D Analysis of chromosome architecture: advantages and limitations with SEM
journal, January 2005

X-ray structure of a tetranucleosome and its implications for the chromatin fibre
journal, July 2005

Three-Dimensional Visualization of a Human Chromosome Using Coherent X-Ray Diffraction
journal, January 2009

The Bionanoprobe: hard X-ray fluorescence nanoprobe with cryogenic capabilities
journal, December 2013

Simultaneous cryo X-ray ptychographic and fluorescence microscopy of green algae
journal, February 2015

Coherent imaging at the Diamond beamline I13
journal, October 2011

Optimization of overlap uniformness for ptychography
journal, January 2014

An improved ptychographical phase retrieval algorithm for diffractive imaging
journal, September 2009

Karyotyping Human Chromosomes by Optical and X-Ray Ptychography Methods
journal, February 2015

EM measurements define the dimensions of the "30-nm" chromatin fiber: Evidence for a compact, interdigitated structure
journal, April 2006

Soft X-ray spectromicroscopy and ptychography
journal, April 2015

Cryo-EM Study of the Chromatin Fiber Reveals a Double Helix Twisted by Tetranucleosomal Units
journal, April 2014

A New Chromosome Model
journal, November 2000

Three-dimensional coherent X-ray diffractive imaging of whole frozen-hydrated cells
journal, August 2015

Cryo FIB-SEM: Volume imaging of cellular ultrastructure in native frozen specimens
journal, November 2013

Electron microscopy of high pressure frozen samples: bridging the gap between cellular ultrastructure and atomic resolution
journal, September 2008

Soft X-ray microscopy with a cryo scanning transmission X-ray microscope: I. Instrumentation, imaging and spectroscopy
journal, January 2000

Tomography of a Cryo-immobilized Yeast Cell Using Ptychographic Coherent X-Ray Diffractive Imaging
journal, November 2015

Chromatin history: our view from the bridge
journal, October 2003

Cryo-electron microscopy of vitreous sections of native biological cells and tissues
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Cryogenic X-Ray Diffraction Microscopy for Biological Samples
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Cryogenic x-ray diffraction microscopy utilizing high-pressure cryopreservation
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Solenoidal model for superstructure in chromatin.
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The future is cold: cryo-preparation methods for transmission electron microscopy of cells
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