skip to main content

DOE PAGESDOE PAGES

Title: Elucidation of roles for vitamin B 12 in regulation of folate, ubiquinone, and methionine metabolism

Only a small fraction of vitamin B 12-requiring organisms are able to synthesize B 12 de novo, making it a common commodity in microbial communities. Initially recognized as an enzyme cofactor of a few enzymes, recent studies have revealed additional B 12-binding enzymes and regulatory roles for B 12. Here we report the development and use of a B 12-based chemical probe to identify B 12-binding proteins in a nonphototrophic B 12-producing bacterium. Two unexpected discoveries resulted from this study. First, we identified a new light-sensing B 12-binding transcriptional regulator and demonstrated that it controls folate and ubiquinone biosynthesis. Second, our probe captured proteins involved in folate, methionine, and ubiquinone metabolism suggesting that it may play a role as an allosteric effector of these processes. These metabolic processes produce precursors for synthesis of DNA, RNA, and protein. Furthermore, B 12 modulates growth, and by limiting its availability to auxotrophs, B 12-producing organisms may facilitate coordination of community metabolism.
Authors:
 [1] ;  [2] ;  [1] ;  [1] ;  [1] ;  [3] ;  [4] ;  [5] ;  [1] ;  [1] ; ORCiD logo [1] ;  [1] ;  [1]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  2. Russian Academy of Sciences, Moscow (Russia); Sanford-Burnham-Prebys Medical Discovery Institute, La Jolla, CA (United States)
  3. Sanford-Burnham-Prebys Medical Discovery Institute, La Jolla, CA (United States); Univ. of California, San Diego, La Jolla, CA (United States)
  4. Polytech Nice-Sophia, Biot (France)
  5. Sanford-Burnham-Prebys Medical Discovery Institute, La Jolla, CA (United States)
Publication Date:
Report Number(s):
PNNL-SA-119181
Journal ID: ISSN 0027-8424; KP1601010
Grant/Contract Number:
AC05-76RL01830; 58612
Type:
Published Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Volume: 114; Journal Issue: 7; Journal ID: ISSN 0027-8424
Publisher:
National Academy of Sciences, Washington, DC (United States)
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; chemical biology; cobalamin; metabolism; microbial regulation
OSTI Identifier:
1341767
Alternate Identifier(s):
OSTI ID: 1347852