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Title: Efficacy of acetate-amended biostimulation for uranium sequestration: Combined analysis of sediment/groundwater geochemistry and bacterial community structure

Systematic flow-through column experiments were conducted using sediments and ground water collected from different subsurface localities at the U.S. Department of Energy's Integrated Field Research Challenge site in Rifle, Colorado. The principal purpose of this study is to gain a better understanding of the interactive effects of groundwater geochemistry, sediment mineralogy, and indigenous bacterial community structures on the efficacy of uranium removal from the groundwater with/without acetate amendment. Overall, we find that the subtle variations in the sediments' mineralogy, redox conditions, as well as contents of metal(loid) co-contaminants showed a pronounced effect on the associated bacterial population and composition, which mainly determines the system's performance with respect to uranium removal. Positive relationship was identified between the abundance of dissimilatory sulfate-reduction genes (i.e., drsA), markers of sulfate-reducing bacteria, and the sediments' propensity to sequester aqueous uranium. In contrast, no obvious connections were observed between the abundance of common iron-reducing bacteria, e.g., Geobacter spp., and the sediments' ability to sequester uranium. In the sediments with low bacterial biomass and the absence of sulfate-reducing conditions, abiotic adsorption onto mineral surfaces such as phyllosilicates likely played a relatively major role in the attenuation of aqueous uranium; however, in these scenarios, acetate amendment induced detectablemore » rebounds in the effluent uranium concentrations. Lastly, the results of this study suggest that immobilization of uranium can be achieved under predominantly sulfate-reducing conditions, and provide insight into the integrated roles of various biogeochemical components in long-term uranium sequestration.« less
Authors:
 [1] ;  [2] ;  [3] ;  [1] ; ORCiD logo [1] ;  [1] ;  [3] ;  [3] ;  [4]
  1. Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States)
  2. Univ. of Glasgow, Scotland (United Kingdom)
  3. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  4. Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Publication Date:
Report Number(s):
PNNL-SA-123123
Journal ID: ISSN 0883-2927; PII: S0883292716306138
Grant/Contract Number:
SC0006825; AC0576RL01830
Type:
Accepted Manuscript
Journal Name:
Applied Geochemistry
Additional Journal Information:
Journal Volume: 78; Journal Issue: C; Journal ID: ISSN 0883-2927
Publisher:
Elsevier
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
58 GEOSCIENCES; uranium bioremediation; sulfate reducing bacteria; bacterial communities
OSTI Identifier:
1341753
Alternate Identifier(s):
OSTI ID: 1419105

Xu, Jie, Veeramani, Harish, Qafoku, Nikolla P., Singh, Gargi, Riquelme, Maria V., Pruden, Amy, Kukkadapu, Ravi K., Gartman, Brandy N., and Hochella, Jr., Michael F.. Efficacy of acetate-amended biostimulation for uranium sequestration: Combined analysis of sediment/groundwater geochemistry and bacterial community structure. United States: N. p., Web. doi:10.1016/j.apgeochem.2016.12.024.
Xu, Jie, Veeramani, Harish, Qafoku, Nikolla P., Singh, Gargi, Riquelme, Maria V., Pruden, Amy, Kukkadapu, Ravi K., Gartman, Brandy N., & Hochella, Jr., Michael F.. Efficacy of acetate-amended biostimulation for uranium sequestration: Combined analysis of sediment/groundwater geochemistry and bacterial community structure. United States. doi:10.1016/j.apgeochem.2016.12.024.
Xu, Jie, Veeramani, Harish, Qafoku, Nikolla P., Singh, Gargi, Riquelme, Maria V., Pruden, Amy, Kukkadapu, Ravi K., Gartman, Brandy N., and Hochella, Jr., Michael F.. 2016. "Efficacy of acetate-amended biostimulation for uranium sequestration: Combined analysis of sediment/groundwater geochemistry and bacterial community structure". United States. doi:10.1016/j.apgeochem.2016.12.024. https://www.osti.gov/servlets/purl/1341753.
@article{osti_1341753,
title = {Efficacy of acetate-amended biostimulation for uranium sequestration: Combined analysis of sediment/groundwater geochemistry and bacterial community structure},
author = {Xu, Jie and Veeramani, Harish and Qafoku, Nikolla P. and Singh, Gargi and Riquelme, Maria V. and Pruden, Amy and Kukkadapu, Ravi K. and Gartman, Brandy N. and Hochella, Jr., Michael F.},
abstractNote = {Systematic flow-through column experiments were conducted using sediments and ground water collected from different subsurface localities at the U.S. Department of Energy's Integrated Field Research Challenge site in Rifle, Colorado. The principal purpose of this study is to gain a better understanding of the interactive effects of groundwater geochemistry, sediment mineralogy, and indigenous bacterial community structures on the efficacy of uranium removal from the groundwater with/without acetate amendment. Overall, we find that the subtle variations in the sediments' mineralogy, redox conditions, as well as contents of metal(loid) co-contaminants showed a pronounced effect on the associated bacterial population and composition, which mainly determines the system's performance with respect to uranium removal. Positive relationship was identified between the abundance of dissimilatory sulfate-reduction genes (i.e., drsA), markers of sulfate-reducing bacteria, and the sediments' propensity to sequester aqueous uranium. In contrast, no obvious connections were observed between the abundance of common iron-reducing bacteria, e.g., Geobacter spp., and the sediments' ability to sequester uranium. In the sediments with low bacterial biomass and the absence of sulfate-reducing conditions, abiotic adsorption onto mineral surfaces such as phyllosilicates likely played a relatively major role in the attenuation of aqueous uranium; however, in these scenarios, acetate amendment induced detectable rebounds in the effluent uranium concentrations. Lastly, the results of this study suggest that immobilization of uranium can be achieved under predominantly sulfate-reducing conditions, and provide insight into the integrated roles of various biogeochemical components in long-term uranium sequestration.},
doi = {10.1016/j.apgeochem.2016.12.024},
journal = {Applied Geochemistry},
number = C,
volume = 78,
place = {United States},
year = {2016},
month = {12}
}