skip to main content

DOE PAGESDOE PAGES

Title: Imaging of Hydrogel Microsphere Structure and Foreign Body Response Based on Endogenous X-Ray Phase Contrast

Transplantation of functional islets encapsulated in stable biomaterials has the potential to cure Type I diabetes. However, the success of these materials requires the ability to understand their stability in vivo. Imaging techniques that enable monitoring of biomaterial performance are critical to further development in the field. In this study, we demonstrate for the first time that X-ray phase contrast (XPC) imaging techniques enable 3D imaging and evaluation of islet volume, alginate hydrogel structure and local soft tissue response. Islets were encapsulated in alginate systems prepared in methods used in clinical trials and implanted in a rodent omentum pouch model as a treatment for type I diabetes. Microbeads were imaged with XPC prior to implantation and following implantation into an omentum pouch. Islets could be identified within alginate beads and the islet volume quantified. Omental adipose tissue could be distinguished from inflammatory regions resulting from implanted beads. Individual beads and the local encapsulation response were visualized and quantifiable. Measurements were in agreement with histology. The 3D structure of the microbeads could be characterized with XPC and failed beads could also be identified. These results point to the substantial potential of XPC as a tool for imaging biomaterials in small animalmore » models.« less
Authors:
 [1] ;  [2] ;  [2] ;  [1] ;  [3] ;  [3] ;  [4] ;  [5] ;  [3] ;  [4] ;  [1]
  1. Illinois Inst. of Technology, Chicago, IL (United States). Dept. of Biomedical Engineering; Edward Hines Jr. VA Hospital, Chicago, IL (United States)
  2. Illinois Inst. of Technology, Chicago, IL (United States). Dept. of Biomedical Engineering
  3. Washington Univ., St. Louis, MO (United States). Dept. of Biomedical Engineering
  4. Wake Forest Inst. of Regenerative Medicine, Winston-Salem, NC (United States)
  5. Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source
Publication Date:
Report Number(s):
BNL-113449-2017-JA
Journal ID: ISSN 1937-3384
Grant/Contract Number:
SC00112704
Type:
Accepted Manuscript
Journal Name:
Tissue Engineering. Part C: Methods
Additional Journal Information:
Journal Volume: 22; Journal Issue: 11; Journal ID: ISSN 1937-3384
Publisher:
Mary Ann Liebert, Inc.
Research Org:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; X-ray phase contrast; microcomputed tomography; hydrogels
OSTI Identifier:
1341702