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Title: Photon counting phosphorescence lifetime imaging with TimepixCam

TimepixCam is a novel fast optical imager based on an optimized silicon pixel sensor with a thin entrance window, and read out by a Timepix ASIC. The 256 x 256 pixel sensor has a time resolution of 15 ns at a sustained frame rate of 10 Hz. We used this sensor in combination with an image intensifier for wide-field time-correlated single photon counting (TCSPC) imaging. We have characterised the photon detection capabilities of this detector system, and employed it on a wide-field epifluorescence microscope to map phosphorescence decays of various iridium complexes with lifetimes of about 1 μs in 200 μm diameter polystyrene beads.
Authors:
ORCiD logo [1] ;  [2] ;  [1] ;  [3]
  1. King's College London, London (United Kingdom). Dept. of Physics
  2. Brookhaven National Lab. (BNL), Upton, NY (United States); Peyton Hall, Princeton, NJ (United States). Dept. of Astrophysical Sciences
  3. Brookhaven National Lab. (BNL), Upton, NY (United States)
Publication Date:
Report Number(s):
BNL-113413-2017-JA
Journal ID: ISSN 0034-6748; KA2301020; TRN: US1700988
Grant/Contract Number:
SC0012704; K015664; 13-006
Type:
Accepted Manuscript
Journal Name:
Review of Scientific Instruments
Additional Journal Information:
Journal Volume: 88; Journal Issue: 1; Journal ID: ISSN 0034-6748
Publisher:
American Institute of Physics (AIP)
Research Org:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org:
USDOE Office of Science (SC), High Energy Physics (HEP) (SC-25)
Country of Publication:
United States
Language:
English
Subject:
79 ASTRONOMY AND ASTROPHYSICS; 47 OTHER INSTRUMENTATION; Time-correlated single photon counting (TCSPC); phosphorescence life-time imaging (PLIM); microchannel plate (MCP); Timepix ASIC
OSTI Identifier:
1341684