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Title: X-ray Phase Contrast Allows Three Dimensional, Quantitative Imaging of Hydrogel Implants

Three dimensional imaging techniques are needed for the evaluation and assessment of biomaterials used for tissue engineering and drug delivery applications. Hydrogels are a particularly popular class of materials for medical applications but are difficult to image in tissue using most available imaging modalities. Imaging techniques based on X-ray Phase Contrast (XPC) have shown promise for tissue engineering applications due to their ability to provide image contrast based on multiple X-ray properties. In this manuscript we describe results using XPC to image a model hydrogel and soft tissue structure. Porous fibrin loaded poly(ethylene glycol) hydrogels were synthesized and implanted in a rodent subcutaneous model. Samples were explanted and imaged with an analyzer-based XPC technique and processed and stained for histology for comparison. Both hydrogel and soft tissues structures could be identified in XPC images. Structure in skeletal muscle adjacent could be visualized and invading fibrovascular tissue could be quantified. In quantitative results, there were no differences between XPC and the gold-standard histological measurements. These results provide evidence of the significant potential of techniques based on XPC for 3D imaging of hydrogel structure and local tissue response.
Authors:
 [1] ;  [1] ;  [1] ;  [2] ;  [3] ;  [4]
  1. Illinois Inst. of Technology, Chicago, IL (United States); Edward Hines Jr. VA Hospital, Hines, IL (United States)
  2. Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source
  3. Washington Univ., St. Louis, MO (United States)
  4. Illinois Inst. of Technology, Chicago, IL (United States)
Publication Date:
Report Number(s):
BNL-113346-2016-JA
Journal ID: ISSN 0090-6964
Grant/Contract Number:
SC00112704
Type:
Accepted Manuscript
Journal Name:
Annals of Biomedical Engineering
Additional Journal Information:
Journal Volume: 44; Journal Issue: 3; Journal ID: ISSN 0090-6964
Publisher:
Springer
Research Org:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; X-ray phase contrast; imaging; hydrogels; tissue engineering; micro-computed tomography
OSTI Identifier:
1341637