A resettable in-line particle concentrator using AC electrokinetics for distributed monitoring of microalgae in source waters
Abstract
Green algae have been studied as an important and effective biomarker to indicate water quality due to their sensitivity to toxic agents in freshwater sources. But, conventional methods to monitor algal physiology use a chlorophyll fluorometer whose use is hampered by high-cost, large footprint, and limited sensitivity for practical samples containing low algal concentration. In order to overcome these constraints, we developed a multi-level electrode platform for resettable trapping of algae via AC electro-osmosis (ACEO) and negative dielectrophoresis. Preliminary experiments were performed in freshwater with conductivity of 0.02 S/m. Algal trapping was demonstrated at a low voltage of 2 V. The concentration effect was experimentally verified by measuring the fluorescence intensity of algae and using hemocytometer counting chambers at the inlet and outlet of the multilevel microchannel lab-on-a-chip. An optimal frequency was found for trapping, which agrees with the frequency dependence of ACEO flow velocity. Through-flow rate and electrode dimensions were optimized as well. Trapping efficiencies within the range of 26% - 65% have been obtained. A maximum trapping rate of 182 cells/s was obtained with a flow rate of 20 l/min. Our lab-on-a-chip shows high potential for improving the limit of detection in algal monitoring and enabling the developmentmore »
- Authors:
-
- Univ. of Tennessee, Knoxville, TN (United States). Dept. of Electrical Engineering and Computer Science
- (United States). Weldon School of Biomedical Engineering
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Chemical Sciences Division
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1341580
- Alternate Identifier(s):
- OSTI ID: 1417103
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Sensors and Actuators. B, Chemical
- Additional Journal Information:
- Journal Volume: 244; Journal Issue: C; Journal ID: ISSN 0925-4005
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 30 DIRECT ENERGY CONVERSION; Microfluidics; AC electrokinetics; in line particle trapping; microalgae; biosensors; source water monitoring
Citation Formats
Yuan, Quan, Purdue Univ., West Lafayette, IN, Wu, Jayne, Greenbaum, Elias, and Evans, Barbara R. A resettable in-line particle concentrator using AC electrokinetics for distributed monitoring of microalgae in source waters. United States: N. p., 2016.
Web. doi:10.1016/j.snb.2016.12.124.
Yuan, Quan, Purdue Univ., West Lafayette, IN, Wu, Jayne, Greenbaum, Elias, & Evans, Barbara R. A resettable in-line particle concentrator using AC electrokinetics for distributed monitoring of microalgae in source waters. United States. https://doi.org/10.1016/j.snb.2016.12.124
Yuan, Quan, Purdue Univ., West Lafayette, IN, Wu, Jayne, Greenbaum, Elias, and Evans, Barbara R. Thu .
"A resettable in-line particle concentrator using AC electrokinetics for distributed monitoring of microalgae in source waters". United States. https://doi.org/10.1016/j.snb.2016.12.124. https://www.osti.gov/servlets/purl/1341580.
@article{osti_1341580,
title = {A resettable in-line particle concentrator using AC electrokinetics for distributed monitoring of microalgae in source waters},
author = {Yuan, Quan and Purdue Univ., West Lafayette, IN and Wu, Jayne and Greenbaum, Elias and Evans, Barbara R.},
abstractNote = {Green algae have been studied as an important and effective biomarker to indicate water quality due to their sensitivity to toxic agents in freshwater sources. But, conventional methods to monitor algal physiology use a chlorophyll fluorometer whose use is hampered by high-cost, large footprint, and limited sensitivity for practical samples containing low algal concentration. In order to overcome these constraints, we developed a multi-level electrode platform for resettable trapping of algae via AC electro-osmosis (ACEO) and negative dielectrophoresis. Preliminary experiments were performed in freshwater with conductivity of 0.02 S/m. Algal trapping was demonstrated at a low voltage of 2 V. The concentration effect was experimentally verified by measuring the fluorescence intensity of algae and using hemocytometer counting chambers at the inlet and outlet of the multilevel microchannel lab-on-a-chip. An optimal frequency was found for trapping, which agrees with the frequency dependence of ACEO flow velocity. Through-flow rate and electrode dimensions were optimized as well. Trapping efficiencies within the range of 26% - 65% have been obtained. A maximum trapping rate of 182 cells/s was obtained with a flow rate of 20 l/min. Our lab-on-a-chip shows high potential for improving the limit of detection in algal monitoring and enabling the development of a portable, integrated and automated system for monitoring the quality of source drinking waters.},
doi = {10.1016/j.snb.2016.12.124},
journal = {Sensors and Actuators. B, Chemical},
number = C,
volume = 244,
place = {United States},
year = {Thu Dec 29 00:00:00 EST 2016},
month = {Thu Dec 29 00:00:00 EST 2016}
}
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Works referenced in this record:
Detection Limits for Real-Time Source Water Monitoring Using Indigenous Freshwater Microalgae
journal, November 2009
- Rodriguez, Miguel; Greenbaum, Elias
- Water Environment Research, Vol. 81, Issue 11
Comparative Sensitivity of Freshwater Algae to Atrazine
journal, January 2006
- Lockert, C. K.; Hoagland, K. D.; Siegfried, B. D.
- Bulletin of Environmental Contamination and Toxicology, Vol. 76, Issue 1
A risk assessment of pollution: induction of atrazine tolerance in phytoplankton communities in freshwater outdoor mesocosms, using chlorophyll fluorescence as an endpoint
journal, July 2002
- Seguin, Florence; Le Bihan, Frédéric; Leboulanger, Christophe
- Water Research, Vol. 36, Issue 13
Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer
journal, January 1986
- Schreiber, U.; Schliwa, U.; Bilger, W.
- Photosynthesis Research, Vol. 10, Issue 1-2
Portable, solid‐state fluorometer for the measurement of chlorophyll fluorescence induction in plants
journal, May 1975
- Schreiber, Ulrich; Groberman, Leslie; Vidaver, William
- Review of Scientific Instruments, Vol. 46, Issue 5
Evaluation of a technique for the measurement of chlorophyll fluorescence from leaves exposed to continuous white light
journal, September 1985
- ÖGren, E.; Baker, N. R.
- Plant, Cell & Environment, Vol. 8, Issue 7
Detection of rapid induction kinetics with a new type of high-frequency modulated chlorophyll fluorometer
journal, January 1986
- Schreiber, U.
- Photosynthesis Research, Vol. 9, Issue 1-2
Applying Pulse Amplitude Modulation (PAM) fluorometry to microalgae suspensions: stirring potentially impacts fluorescence
journal, June 2006
- Cosgrove, Jeffrey; Borowitzka, Michael
- Photosynthesis Research, Vol. 88, Issue 3
A laser diode fluorometer for field measurements of the F685/F730 chlorophyll fluorescence ratio
journal, October 1996
- Mazzinghi, P.
- Review of Scientific Instruments, Vol. 67, Issue 10
Two-wavelength, multipurpose, truly portable chlorophyll fluorometer and its application in field monitoring of phytoremediation
journal, May 2000
- Barócsi, A.; Kocsányi, L.; Várkonyi, S.
- Measurement Science and Technology, Vol. 11, Issue 6
Optical trapping and manipulation of single cells using infrared laser beams
journal, December 1987
- Ashkin, A.; Dziedzic, J. M.; Yamane, T.
- Nature, Vol. 330, Issue 6150
Creating permanent 3D arrangements of isolated cells using holographic optical tweezers
journal, January 2005
- Jordan, Pamela; Leach, Jonathan; Padgett, Miles
- Lab on a Chip, Vol. 5, Issue 11
Evidence for localized cell heating induced by infrared optical tweezers
journal, May 1995
- Liu, Y.; Cheng, D. K.; Sonek, G. J.
- Biophysical Journal, Vol. 68, Issue 5
Cell docking inside microwells within reversibly sealed microfluidic channels for fabricating multiphenotype cell arrays
journal, January 2005
- Khademhosseini, Ali; Yeh, Judy; Eng, George
- Lab on a Chip, Vol. 5, Issue 12
A Microfluidic Manipulator for Enrichment and Alignment of Moving Cells and Particles
journal, June 2009
- Chen, Hsiu-hung; Sun, Bingbing; Tran, Kenny K.
- Journal of Biomechanical Engineering, Vol. 131, Issue 7
Stable hydrodynamic trapping of hydrogel beads for on-chip differentiation analysis of encapsulated stem cells
journal, May 2012
- Kim, Choong; Bang, Jae Hoon; Kim, Young Eun
- Sensors and Actuators B: Chemical, Vol. 166-167
Hydrodynamic filtration for on-chip particle concentration and classification utilizing microfluidics
journal, January 2005
- Yamada, Masumi; Seki, Minoru
- Lab on a Chip, Vol. 5, Issue 11
Micropatterning of Cells Using Modulated Magnetic Fields
journal, February 2005
- Kimura, Tsunehisa; Sato, Yukiko; Kimura, Fumiko
- Langmuir, Vol. 21, Issue 3
Capture of DNA in microfluidic channel using magnetic beads: Increasing capture efficiency with integrated microfluidic mixer
journal, April 2007
- Lund-Olesen, T.; Dufva, M.; Hansen, M. F.
- Journal of Magnetism and Magnetic Materials, Vol. 311, Issue 1
Manipulation of biological cells using a microelectromagnet matrix
journal, August 2004
- Lee, H.; Purdon, A. M.; Westervelt, R. M.
- Applied Physics Letters, Vol. 85, Issue 6
Label-free cellular manipulation and sorting via biocompatible ferrofluids
journal, December 2009
- Kose, A. R.; Fischer, B.; Mao, L.
- Proceedings of the National Academy of Sciences, Vol. 106, Issue 51
Noninvasive Acoustic Cell Trapping in a Microfluidic Perfusion System for Online Bioassays
journal, April 2007
- Evander, Mikael; Johansson, Linda; Lilliehorn, Tobias
- Analytical Chemistry, Vol. 79, Issue 7
Non-contact acoustic cell trapping in disposable glass capillaries
journal, January 2010
- Hammarström, Björn; Evander, Mikael; Barbeau, Herve
- Lab on a Chip, Vol. 10, Issue 17
Microdevices for dielectrophoretic flow-through cell separation
journal, November 2003
- Holmes, D.; Green, N. G.; Morgan, H.
- IEEE Engineering in Medicine and Biology Magazine, Vol. 22, Issue 6
Contactless dielectrophoresis: a new technique for cell manipulation
journal, May 2009
- Shafiee, Hadi; Caldwell, John L.; Sano, Michael B.
- Biomedical Microdevices, Vol. 11, Issue 5
Dielectrophoretic responses of DNA and fluorophore in physiological solution by impedimetric characterization
journal, March 2013
- Li, Shanshan; Yuan, Quan; Morshed, Bashir I.
- Biosensors and Bioelectronics, Vol. 41
Dielectrophoresis in Microchips Containing Arrays of Insulating Posts: Theoretical and Experimental Results
journal, September 2003
- Cummings, Eric B.; Singh, Anup K.
- Analytical Chemistry, Vol. 75, Issue 18
Dielectrophoretic Concentration and Separation of Live and Dead Bacteria in an Array of Insulators
journal, March 2004
- Lapizco-Encinas, Blanca H.; Simmons, Blake A.; Cummings, Eric B.
- Analytical Chemistry, Vol. 76, Issue 6
Selective trapping of single mammalian breast cancer cells by insulator-based dielectrophoresis
journal, January 2014
- Bhattacharya, Sanchari; Chao, Tzu-Chiao; Ariyasinghe, Nethmi
- Analytical and Bioanalytical Chemistry, Vol. 406, Issue 7
Interactions of electrical fields with fluids:laboratory-on-a-chip applications
journal, January 2008
- Wu, J.
- IET Nanobiotechnology, Vol. 2, Issue 1
AC Electric-Field-Induced Fluid Flow in Microelectrodes
journal, September 1999
- Ramos, Antonio; Morgan, Hywel; Green, Nicolas G.
- Journal of Colloid and Interface Science, Vol. 217, Issue 2
Particle detection by electrical impedance spectroscopy with asymmetric-polarization AC electroosmotic trapping
journal, April 2005
- Wu, Jie; Ben, Yuxing; Chang, Hsueh-Chia
- Microfluidics and Nanofluidics, Vol. 1, Issue 2
Long-Range AC Electroosmotic Trapping and Detection of Bioparticles
journal, April 2005
- Wu, Jie; Ben, Yuxing; Battigelli, David
- Industrial & Engineering Chemistry Research, Vol. 44, Issue 8
A Simple Method to Integrate In Situ Nano-Particle Focusing With Cantilever Detection
journal, June 2007
- Wu, Jie; Islam, Nazmul
- IEEE Sensors Journal, Vol. 7, Issue 6
Enhancing microcantilever capability with integrated AC electroosmotic trapping
journal, November 2006
- Islam, N.; Lian, M.; Wu, J.
- Microfluidics and Nanofluidics, Vol. 3, Issue 3
AC electrokinetics-enhanced capacitive immunosensor for point-of-care serodiagnosis of infectious diseases
journal, January 2014
- Li, Shanshan; Cui, Haochen; Yuan, Quan
- Biosensors and Bioelectronics, Vol. 51
AC electroosmotic micromixer for chemical processing in a microchannel
journal, January 2006
- Sasaki, Naoki; Kitamori, Takehiko; Kim, Haeng-Boo
- Lab on a Chip, Vol. 6, Issue 4
AC electroosmotic generated in-plane microvortices for stationary or continuous fluid mixing
journal, July 2007
- Huang, Shih-Hao; Wang, Shou-Kai; Khoo, Hwa Seng
- Sensors and Actuators B: Chemical, Vol. 125, Issue 1
DC-biased AC-electroosmotic and AC-electrothermal flow mixing in microchannels
journal, January 2009
- Ng, Wee Yang; Goh, Shireen; Lam, Yee Cheong
- Lab Chip, Vol. 9, Issue 6
Assessment of three AC electroosmotic flow protocols for mixing in microfluidic channel
journal, January 2009
- Chen, Jia-Kun; Weng, Chi-Neng; Yang, Ruey-Jen
- Lab on a Chip, Vol. 9, Issue 9
AC electrothermal manipulation of conductive fluids and particles for lab-chip applications
journal, January 2007
- Lian, M.; Islam, N.; Wu, J.
- IET Nanobiotechnology, Vol. 1, Issue 3
Optimization of planar interdigitated microelectrode array for biofluid transport by AC electrothermal effect
journal, July 2013
- Yuan, Quan; Yang, Kai; Wu, Jie
- Microfluidics and Nanofluidics, Vol. 16, Issue 1-2
Investigation of microflow reversal by ac electrokinetics in orthogonal electrodes for micropump design
journal, June 2008
- Yang, Kai; Wu, Jie
- Biomicrofluidics, Vol. 2, Issue 2
Mitotic Replication of Deoxyribonucleic acid in Chlamydomonas Reinhardi
journal, January 1960
- Sueoka, N.
- Proceedings of the National Academy of Sciences, Vol. 46, Issue 1
Spectrometric Assays for Plant, Algal and Bacterial Chlorophylls
book, January 2006
- Porra, Robert J.
- Chlorophylls and Bacteriochlorophylls: Biochemistry, Biophysics, Functions and Applications
Bisphenol A Sensors on Polyimide Fabricated by Laser Direct Writing for Onsite River Water Monitoring at Attomolar Concentration
journal, July 2016
- Cheng, Cheng; Wang, Shutong; Wu, Jayne
- ACS Applied Materials & Interfaces, Vol. 8, Issue 28
Works referencing / citing this record:
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journal, December 2018
- Zhang, Jian; Wang, Jian; Wu, Jayne
- Microfluidics and Nanofluidics, Vol. 23, Issue 1