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Title: Crystallization and x-ray diffraction analysis of a putative bacterial class I labdane-related diterpene synthase [Crysallization and preliminary x-ray diffraction analysis of a bacterial class I labdane-related diterpene synthase]

Here, labdane-related diterpenoids are natural products with potential pharmaceutical applications that are rarely found in bacteria. Here, a putative class I labdane-related diterpene synthase (LrdC) identified by genome mining in a streptomycete was successfully crystallized using the microbatch method. Crystals of the LrdC enzyme were obtained in a holo form with its natural cofactor Mg 2+ (LrdC-Mg 2+) and in complex with inorganic pyrophosphate (PP i) (LrdC-Mg 2+–PP i). Crystals of native LrdC-Mg 2+ diffracted to 2.50 Å resolution and belonged to the trigonal space group P3 221, with unit-cell parameters a = b = 107.1, c = 89.2 Å. Crystals of the LrdC-Mg 2+–PP i complex grown in the same conditions as the native enzyme with PEG 8000 diffracted to 2.36 Å resolution and also belonged to the trigonal space group P3 221. Crystals of the LrdC-Mg 2+–PP i complex grown in a second crystallization condition with PEG 3350 diffracted to 2.57 Å resolution and belonged to the monoclinic space group P2 1, with unit-cell parameters a = 49.9, b = 104.1, c = 66.5 Å, β = 111.4°. The structure was determined by the single-wavelength anomalous dispersion (SAD) technique using the osmium signal from a potassium hexachloroosmate (IV)more » derivative.« less
Authors:
 [1] ;  [1] ;  [2] ;  [3] ;  [1] ;  [2] ;  [1]
  1. Ciudad Univ. (Mexico)
  2. Univ. Nacional Autonoma de Mexico (Mexico)
  3. Brookhaven National Lab. (BNL), Upton, NY (United States)
Publication Date:
Report Number(s):
BNL-108588-2015-JA
Journal ID: ISSN 2053-230X; ACSFEN; R&D Project: LS001
Grant/Contract Number:
SC00112704
Type:
Accepted Manuscript
Journal Name:
Acta Crystallographica. Section F, Structural Biology Communications
Additional Journal Information:
Journal Volume: 71; Journal Issue: 9; Journal ID: ISSN 2053-230X
Publisher:
International Union of Crystallography
Research Org:
Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source II (NSLS-II)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; National Synchrotron Light Source II; diterpene synthase; genome mining; labdane-related diterpenoid; Streptomyces
OSTI Identifier:
1341511

Serrano-Posada, Hugo, Centeno-Leija, Sara, Rojas-Trejo, Sonia, Stojanoff, Vivian, Rodriguez-Sanoja, Romina, Rudino-Pinera, Enrique, and Sanchez, Sergio. Crystallization and x-ray diffraction analysis of a putative bacterial class I labdane-related diterpene synthase [Crysallization and preliminary x-ray diffraction analysis of a bacterial class I labdane-related diterpene synthase]. United States: N. p., Web. doi:10.1107/S2053230X15014363.
Serrano-Posada, Hugo, Centeno-Leija, Sara, Rojas-Trejo, Sonia, Stojanoff, Vivian, Rodriguez-Sanoja, Romina, Rudino-Pinera, Enrique, & Sanchez, Sergio. Crystallization and x-ray diffraction analysis of a putative bacterial class I labdane-related diterpene synthase [Crysallization and preliminary x-ray diffraction analysis of a bacterial class I labdane-related diterpene synthase]. United States. doi:10.1107/S2053230X15014363.
Serrano-Posada, Hugo, Centeno-Leija, Sara, Rojas-Trejo, Sonia, Stojanoff, Vivian, Rodriguez-Sanoja, Romina, Rudino-Pinera, Enrique, and Sanchez, Sergio. 2015. "Crystallization and x-ray diffraction analysis of a putative bacterial class I labdane-related diterpene synthase [Crysallization and preliminary x-ray diffraction analysis of a bacterial class I labdane-related diterpene synthase]". United States. doi:10.1107/S2053230X15014363. https://www.osti.gov/servlets/purl/1341511.
@article{osti_1341511,
title = {Crystallization and x-ray diffraction analysis of a putative bacterial class I labdane-related diterpene synthase [Crysallization and preliminary x-ray diffraction analysis of a bacterial class I labdane-related diterpene synthase]},
author = {Serrano-Posada, Hugo and Centeno-Leija, Sara and Rojas-Trejo, Sonia and Stojanoff, Vivian and Rodriguez-Sanoja, Romina and Rudino-Pinera, Enrique and Sanchez, Sergio},
abstractNote = {Here, labdane-related diterpenoids are natural products with potential pharmaceutical applications that are rarely found in bacteria. Here, a putative class I labdane-related diterpene synthase (LrdC) identified by genome mining in a streptomycete was successfully crystallized using the microbatch method. Crystals of the LrdC enzyme were obtained in a holo form with its natural cofactor Mg2+ (LrdC-Mg2+) and in complex with inorganic pyrophosphate (PPi) (LrdC-Mg2+–PPi). Crystals of native LrdC-Mg2+ diffracted to 2.50 Å resolution and belonged to the trigonal space group P3221, with unit-cell parameters a = b = 107.1, c = 89.2 Å. Crystals of the LrdC-Mg2+–PPi complex grown in the same conditions as the native enzyme with PEG 8000 diffracted to 2.36 Å resolution and also belonged to the trigonal space group P3221. Crystals of the LrdC-Mg2+–PPi complex grown in a second crystallization condition with PEG 3350 diffracted to 2.57 Å resolution and belonged to the monoclinic space group P21, with unit-cell parameters a = 49.9, b = 104.1, c = 66.5 Å, β = 111.4°. The structure was determined by the single-wavelength anomalous dispersion (SAD) technique using the osmium signal from a potassium hexachloroosmate (IV) derivative.},
doi = {10.1107/S2053230X15014363},
journal = {Acta Crystallographica. Section F, Structural Biology Communications},
number = 9,
volume = 71,
place = {United States},
year = {2015},
month = {8}
}