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Title: A flexible microbial co-culture platform for simultaneous utilization of methane and carbon dioxide from gas feedstocks

A new co-cultivation technology is presented that converts greenhouse gasses, CH 4 and CO 2, into microbial biomass. The methanotrophic bacterium, Methylomicrobium alcaliphilum 20z, was coupled to a cyanobacterium, Synechococcus PCC 7002 via oxygenic photosynthesis. The system exhibited robust growth on diverse gas mixtures ranging from biogas to those representative of a natural gas feedstock. A continuous processes was developed on a synthetic natural gas feed that achieved steady-state by imposing coupled light and O 2 limitations on the cyanobacterium and methanotroph, respectively. Continuous co-cultivation resulted in an O 2 depleted reactor and does not require CH 4/O 2 mixtures to be fed into the system, thereby enhancing process safety considerations over traditional methanotroph mono-culture platforms. This co-culture technology is scalable with respect to its ability to utilize different gas streams and its biological components constructed from model bacteria that can be metabolically customized to produce a range of biofuels and bioproducts.
 [1] ;  [1] ;  [1] ;  [2]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division; Washington State Univ., Pullman, WA (United States). The Gene and Linda Voiland School of Chemical Engineering and Bioengineering
Publication Date:
Report Number(s):
Journal ID: ISSN 0960-8524; PII: S0960852417300056
Grant/Contract Number:
Published Article
Journal Name:
Bioresource Technology
Additional Journal Information:
Journal Volume: 228; Journal ID: ISSN 0960-8524
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); USDOE Office of Energy Efficiency and Renewable Energy (EERE)
Country of Publication:
United States
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; methane; carbon dioxide; co-culture; biogas; natural gas; methanotroph; cyanobacteria; Bacteria; Environmental Molecular Sciences Laboratory
OSTI Identifier:
Alternate Identifier(s):
OSTI ID: 1341742; OSTI ID: 1347845