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Title: Distribution of iron oxide core-titanium dioxide shell nanoparticles in VX2 tumor bearing rabbits introduced by two different delivery modalities

Abstract

This work compares intravenous (IV) versus fluoroscopy-guided transarterial intra-catheter (IC) delivery of iron oxide core-titanium dioxide shell nanoparticles (NPs) in vivo in VX2 model of liver cancer in rabbits. NPs coated with glucose and decorated with a peptide sequence from cortactin were administered to animals with developed VX2 liver cancer. Two hours after NPs delivery tumors, normal liver, kidney, lung and spleen tissues were harvested and used for a series on histological and elemental analysis tests. Quantification of NPs in tissues was done both by bulk inductively coupled plasma mass spectrometry (ICP-MS) analysis and by hard X-ray fluorescence microscopy. Both IV and IC NPs injection are feasible modalities for delivering NPs to VX2 liver tumors with comparable tumor accumulation. It is possible that this is an outcome of the fact that VX2 tumors are highly vascularized and hemorrhagic, and therefore enhanced permeability and retention (EPR) plays the most significant role in accumulation of nanoparticles in tumor tissue. It is, however, interesting to note that IV delivery led to increased sequestration of NPs by spleen and normal liver tissue, while IC delivery lead to more NP positive Kupffer cells. Furthermore, this difference is most likely a direct outcome of blood flowmore » dynamics. Armed with this knowledge about nanoparticle delivery, we plan to test them as radiosensitizers in the future.« less

Authors:
 [1];  [2];  [3];  [2];  [2];  [2];  [2];  [2];  [2];  [4];  [4];  [4];  [5];  [2];  [2];  [2];  [2]
  1. Northwestern Univ., Chicago, IL (United States); Alexandria Univ., Alexandria (Egypt)
  2. Northwestern Univ., Chicago, IL (United States)
  3. Alexandria Univ., Alexandria (Egypt)
  4. Argonne National Lab. (ANL), Argonne, IL (United States)
  5. Vanderbilt Univ. School of Medicine, Nashville, TN (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Institutes of Health (NIH); U.S. Army Research Laboratory, U.S. Army Research Office (ARO)
OSTI Identifier:
1341018
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Accepted Manuscript
Journal Name:
Nanomaterials
Additional Journal Information:
Journal Volume: 6; Journal Issue: 8; Journal ID: ISSN 2079-4991
Publisher:
MDPI
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; core–shell nanoparticle; rabbit VX2 liver cancer model; transarterial intra-catheter delivery

Citation Formats

Refaat, Tamer, West, Derek, El Achy, Samar, Parimi, Vamsi, May, Jasmine, Xin, Lun, Harris, Kathleen R., Liu, William, Wanzer, Michael Beau, Finney, Lydia, Maxey, Evan, Vogt, Stefan, Omary, Reed A., Procissi, Daniele, Larson, Andrew C., Paunesku, Tatjana, and Woloschak, Gayle E. Distribution of iron oxide core-titanium dioxide shell nanoparticles in VX2 tumor bearing rabbits introduced by two different delivery modalities. United States: N. p., 2016. Web. doi:10.3390/nano6080143.
Refaat, Tamer, West, Derek, El Achy, Samar, Parimi, Vamsi, May, Jasmine, Xin, Lun, Harris, Kathleen R., Liu, William, Wanzer, Michael Beau, Finney, Lydia, Maxey, Evan, Vogt, Stefan, Omary, Reed A., Procissi, Daniele, Larson, Andrew C., Paunesku, Tatjana, & Woloschak, Gayle E. Distribution of iron oxide core-titanium dioxide shell nanoparticles in VX2 tumor bearing rabbits introduced by two different delivery modalities. United States. doi:10.3390/nano6080143.
Refaat, Tamer, West, Derek, El Achy, Samar, Parimi, Vamsi, May, Jasmine, Xin, Lun, Harris, Kathleen R., Liu, William, Wanzer, Michael Beau, Finney, Lydia, Maxey, Evan, Vogt, Stefan, Omary, Reed A., Procissi, Daniele, Larson, Andrew C., Paunesku, Tatjana, and Woloschak, Gayle E. Wed . "Distribution of iron oxide core-titanium dioxide shell nanoparticles in VX2 tumor bearing rabbits introduced by two different delivery modalities". United States. doi:10.3390/nano6080143. https://www.osti.gov/servlets/purl/1341018.
@article{osti_1341018,
title = {Distribution of iron oxide core-titanium dioxide shell nanoparticles in VX2 tumor bearing rabbits introduced by two different delivery modalities},
author = {Refaat, Tamer and West, Derek and El Achy, Samar and Parimi, Vamsi and May, Jasmine and Xin, Lun and Harris, Kathleen R. and Liu, William and Wanzer, Michael Beau and Finney, Lydia and Maxey, Evan and Vogt, Stefan and Omary, Reed A. and Procissi, Daniele and Larson, Andrew C. and Paunesku, Tatjana and Woloschak, Gayle E.},
abstractNote = {This work compares intravenous (IV) versus fluoroscopy-guided transarterial intra-catheter (IC) delivery of iron oxide core-titanium dioxide shell nanoparticles (NPs) in vivo in VX2 model of liver cancer in rabbits. NPs coated with glucose and decorated with a peptide sequence from cortactin were administered to animals with developed VX2 liver cancer. Two hours after NPs delivery tumors, normal liver, kidney, lung and spleen tissues were harvested and used for a series on histological and elemental analysis tests. Quantification of NPs in tissues was done both by bulk inductively coupled plasma mass spectrometry (ICP-MS) analysis and by hard X-ray fluorescence microscopy. Both IV and IC NPs injection are feasible modalities for delivering NPs to VX2 liver tumors with comparable tumor accumulation. It is possible that this is an outcome of the fact that VX2 tumors are highly vascularized and hemorrhagic, and therefore enhanced permeability and retention (EPR) plays the most significant role in accumulation of nanoparticles in tumor tissue. It is, however, interesting to note that IV delivery led to increased sequestration of NPs by spleen and normal liver tissue, while IC delivery lead to more NP positive Kupffer cells. Furthermore, this difference is most likely a direct outcome of blood flow dynamics. Armed with this knowledge about nanoparticle delivery, we plan to test them as radiosensitizers in the future.},
doi = {10.3390/nano6080143},
journal = {Nanomaterials},
number = 8,
volume = 6,
place = {United States},
year = {2016},
month = {8}
}

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Works referenced in this record:

The roles of K+ channels in cancer
journal, December 2013

  • Pardo, Luis A.; Stühmer, Walter
  • Nature Reviews Cancer, Vol. 14, Issue 1
  • DOI: 10.1038/nrc3635

Methods for assessing DNA hybridization of peptide nucleic acid–titanium dioxide nanoconjugates
journal, December 2008

  • Brown, Eric M. B.; Paunesku, Tatjana; Wu, AiGuo
  • Analytical Biochemistry, Vol. 383, Issue 2
  • DOI: 10.1016/j.ab.2008.08.020

Biology of TiO2–oligonucleotide nanocomposites
journal, April 2003

  • Paunesku, Tatjana; Rajh, Tijana; Wiederrecht, Gary
  • Nature Materials, Vol. 2, Issue 5
  • DOI: 10.1038/nmat875

DNA−TiO 2 Nanoconjugates Labeled with Magnetic Resonance Contrast Agents
journal, December 2007

  • Endres, Paul J.; Paunesku, Tatjana; Vogt, Stefan
  • Journal of the American Chemical Society, Vol. 129, Issue 51
  • DOI: 10.1021/ja0772389

Endocytosis of titanium dioxide nanoparticles in prostate cancer PC-3M cells
journal, April 2011

  • Thurn, Kenneth T.; Arora, Hans; Paunesku, Tatjana
  • Nanomedicine: Nanotechnology, Biology and Medicine, Vol. 7, Issue 2
  • DOI: 10.1016/j.nano.2010.09.004

Nanocarriers Enhance Doxorubicin Uptake in Drug-Resistant Ovarian Cancer Cells
journal, December 2011


X-Ray Fluorescence Microscopy for Investigation of Archival Tissues
journal, January 2012


Cytotoxicity and DNA cleavage with core–shell nanocomposites functionalized by a KH domain DNA binding peptide
journal, January 2013

  • Bazak, Remon; Ressl, Jan; Raha, Sumita
  • Nanoscale, Vol. 5, Issue 23
  • DOI: 10.1039/c3nr02203j

Gadolinium-conjugated TiO2-DNA oligonucleotide nanoconjugates show prolonged intracellular retention period and T1-weighted contrast enhancement in magnetic resonance images
journal, September 2008

  • Paunesku, Tatjana; Ke, Tianyi; Dharmakumar, Rohan
  • Nanomedicine: Nanotechnology, Biology and Medicine, Vol. 4, Issue 3
  • DOI: 10.1016/j.nano.2008.04.004