Influence of silica matrix composition and functional component additives on the bioactivity and viability of encapsulated living cells

doi:10.1021/acsbiomaterials.5b00261

Title: Influence of silica matrix composition and functional component additives on the bioactivity and viability of encapsulated living cells

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Abstract

The remarkable impact encapsulation matrix chemistry can have on the bioactivity and viability of integrated living cells is reported. Two silica chemistries (aqueous silicate and alkoxysilane), and a functional component additive (glycerol), are employed to generate three distinct silica matrices. These matrices are used to encapsulate living E. coli cells engineered with a synthetic riboswitch for cell-based biosensing. Following encapsulation, membrane integrity, reproductive capability, and riboswitch-based protein expression levels and rates are measured over a 5 week period. Striking differences in E. coli bioactivity, viability, and biosensing performance are observed for cells encapsulated within the different matrices. E. coli cells encapsulated for 35 days in aqueous silicate-based (AqS) matrices showed relatively low membrane integrity, but high reproductive capability in comparison to cells encapsulated in glycerol containing sodium silicate-based (AqS + g) and alkoxysilane-based (PGS) gels. Further, cells in sodium silicate-based matrices showed increasing fluorescence output over time, resulting in a 1.8-fold higher fluorescence level, and a faster expression rate, over cells free in solution. Furthermore, this unusual and unique combination of biological properties demonstrates that careful design of the encapsulation matrix chemistry can improve functionality of the biocomposite material, and result in new and unexpected physiological states.

Authors:

Savage, Travis J. ^[1]; Dunphy, Darren R. ^[1]; Harbaugh, Svetlana ^[2]; Kelley-Loughnane, Nancy ^[2]; Harper, Jason C. ^[3]; Brinker, C. Jeffrey ^[4]

Univ. of New Mexico, Albuquerque, NM (United States)
Wright Patterson Air Force Base, Dayton, OH (United States)
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Univ. of New Mexico, Albuquerque, NM (United States); Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Publication Date:: 2015-11-06

Research Org.:: Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

OSTI Identifier:: 1340691

Report Number(s):: SAND-2017-0555J
Journal ID: ISSN 2373-9878; 650542

Grant/Contract Number:: AC04-94AL85000

Resource Type:: Accepted Manuscript

Journal Name:: ACS Biomaterials Science & Engineering

Additional Journal Information:: Journal Volume: 1; Journal Issue: 12; Journal ID: ISSN 2373-9878

Publisher:: American Chemical Society (ACS)

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; bioactivity; cell encapsulation; cell viability; glycerol modified silanes; living hybrid biomaterials; whole-cell-based biosensors

Citation Formats


                    Savage, Travis J., Dunphy, Darren R., Harbaugh, Svetlana, Kelley-Loughnane, Nancy, Harper, Jason C., and Brinker, C. Jeffrey. Influence of silica matrix composition and functional component additives on the bioactivity and viability of encapsulated living cells.  United States: N. p., 2015. 
        Web.  doi:10.1021/acsbiomaterials.5b00261.

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                    Savage, Travis J., Dunphy, Darren R., Harbaugh, Svetlana, Kelley-Loughnane, Nancy, Harper, Jason C., & Brinker, C. Jeffrey. Influence of silica matrix composition and functional component additives on the bioactivity and viability of encapsulated living cells.  United States.  doi:10.1021/acsbiomaterials.5b00261.

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                    Savage, Travis J., Dunphy, Darren R., Harbaugh, Svetlana, Kelley-Loughnane, Nancy, Harper, Jason C., and Brinker, C. Jeffrey. Fri .  
        "Influence of silica matrix composition and functional component additives on the bioactivity and viability of encapsulated living cells".  United States.  doi:10.1021/acsbiomaterials.5b00261.  https://www.osti.gov/servlets/purl/1340691.

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@article{osti_1340691,

  title        = {Influence of silica matrix composition and functional component additives on the bioactivity and viability of encapsulated living cells},

  author       = {Savage, Travis J. and Dunphy, Darren R. and Harbaugh, Svetlana and Kelley-Loughnane, Nancy and Harper, Jason C. and Brinker, C. Jeffrey},

  abstractNote = {The remarkable impact encapsulation matrix chemistry can have on the bioactivity and viability of integrated living cells is reported. Two silica chemistries (aqueous silicate and alkoxysilane), and a functional component additive (glycerol), are employed to generate three distinct silica matrices. These matrices are used to encapsulate living E. coli cells engineered with a synthetic riboswitch for cell-based biosensing. Following encapsulation, membrane integrity, reproductive capability, and riboswitch-based protein expression levels and rates are measured over a 5 week period. Striking differences in E. coli bioactivity, viability, and biosensing performance are observed for cells encapsulated within the different matrices. E. coli cells encapsulated for 35 days in aqueous silicate-based (AqS) matrices showed relatively low membrane integrity, but high reproductive capability in comparison to cells encapsulated in glycerol containing sodium silicate-based (AqS + g) and alkoxysilane-based (PGS) gels. Further, cells in sodium silicate-based matrices showed increasing fluorescence output over time, resulting in a 1.8-fold higher fluorescence level, and a faster expression rate, over cells free in solution. Furthermore, this unusual and unique combination of biological properties demonstrates that careful design of the encapsulation matrix chemistry can improve functionality of the biocomposite material, and result in new and unexpected physiological states.},

  doi          = {10.1021/acsbiomaterials.5b00261},

  journal      = {ACS Biomaterials Science & Engineering},

  number       = 12,

  volume       = 1,

  place        = {United States},

  year         = {2015},

  month        = {11}

}

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DOI: 10.1021/acsbiomaterials.5b00261

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